Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI10E1
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Product Specifications
Immunogen
Full-length protein expressed in 293T cell transfected with human MAPK12 expression vector
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
This antibody is specific for Homo sapiens mitogen-activated protein kinase 12 (MAPK12).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for p38 gamma/SAPK3 Antibody (OTI10E1)
![Western Blot: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]](https://resources.rndsystems.com/images/products/p38-gamma-SAPK3-Antibody-10E1-Western-Blot-NBP1-47852-img0008.jpg "Western Blot: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]")
Western Blot: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]
Western Blot: p38 gamma/SAPK3 Antibody (10E1) [NBP1-47852] - hHEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAPK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAPK3.![Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]](https://resources.rndsystems.com/images/products/p38-gamma-SAPK3-Antibody-10E1-Immunohistochemistry-Paraffin-NBP1-47852-img0007.jpg "Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]")
Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]
Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (10E1) [NBP1-47852] - Staining of paraffin-embedded Human pancreas tissue using anti-SAPK3 mouse monoclonal antibody.![Flow Cytometry: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]](https://resources.rndsystems.com/images/products/p38-gamma-SAPK3-Antibody-10E1-Flow-Cytometry-NBP1-47852-img0005.jpg "Flow Cytometry: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]")
Flow Cytometry: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]
Flow Cytometry: p38 gamma/SAPK3 Antibody (10E1) [NBP1-47852] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SAPK3 antibody, and then analyzed by flow cytometry.![Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]](https://resources.rndsystems.com/images/products/p38-gamma-SAPK3-Antibody-10E1-Immunohistochemistry-Paraffin-NBP1-47852-img0006.jpg "Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]")
Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]
Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (10E1) [NBP1-47852] - Staining of paraffin-embedded Human breast tissue using anti-SAPK3 mouse monoclonal antibody.Applications for p38 gamma/SAPK3 Antibody (OTI10E1)
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:500
Reviewed Applications
Read 1 review rated 5 using NBP1-47852 in the following applications:
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: p38 gamma
Additional p38 gamma Products
Product Documents for p38 gamma/SAPK3 Antibody (OTI10E1)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for p38 gamma/SAPK3 Antibody (OTI10E1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for p38 gamma/SAPK3 Antibody (OTI10E1) (1)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
IL-17 Family Signaling Pathways
Inflammasome Activation Pathways
MAPK Signaling: Oxidative Stress Pathway
NOD-like Receptor Signaling Pathways
Pathogen or Damage-activated C-Type Lectin Receptor Signaling Pathways
TGF-beta Signaling Pathways
Toll-Like Receptor Signaling Pathways
VEGF - VEGF R2 Signaling Pathways
TGF-beta Signaling Pathways
Toll-Like Receptor Signaling Pathways
VEGF - VEGF R2 Signaling Pathways