p53 Antibody (CL2199) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-43954
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL2199
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P04637, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse p53 Antibody (CL2199) - Azide and BSA Free (NBP2-34495) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for p53 Antibody (CL2199) - Azide and BSA Free
![p53 Antibody (CL2199) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP3-43954]](https://beta-resources.rndsystems.com/images/products/nbp3-43954_mouse-p53-mab-cl2199-azide-and-bsa-free-282202512115324.jpg "Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP3-43954]")
Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP3-43954]
Staining in A431 cell line with Anti-p53 monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).![p53 Antibody (CL2199) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP3-43954]](https://beta-resources.rndsystems.com/images/products/nbp3-43954_mouse-p53-mab-cl2199-azide-and-bsa-free-282202512163632.jpg "Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP3-43954]")
Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP3-43954]
Staining in MCF7 cell line with Anti-p53 monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).![p53 Antibody (CL2199) - Azide and BSA Free Immunohistochemistry-Paraffin: p53 Antibody [NBP3-43954]](https://beta-resources.rndsystems.com/images/products/nbp3-43954_mouse-p53-mab-cl2199-azide-and-bsa-free-2822025129121.jpg "Immunohistochemistry-Paraffin: p53 Antibody [NBP3-43954]")
![p53 Antibody (CL2199) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP3-43954]](https://beta-resources.rndsystems.com/images/products/nbp3-43954_mouse-p53-mab-cl2199-azide-and-bsa-free-28220251211535.jpg "Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP3-43954]")
Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP3-43954]
Staining in U251 cell line with Anti-p53 monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).![p53 Antibody (CL2199) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP3-43954]](https://beta-resources.rndsystems.com/images/products/nbp3-43954_mouse-p53-mab-cl2199-azide-and-bsa-free-28220251210184.jpg "Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP3-43954]")
Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP3-43954]
Staining in U2OS cell line with Anti-p53 monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).![p53 Antibody (CL2199) - Azide and BSA Free Western Blot: p53 Antibody [NBP3-43954]](https://beta-resources.rndsystems.com/images/products/nbp3-43954_mouse-p53-mab-cl2199-azide-and-bsa-free-28220251217317.jpg "Western Blot: p53 Antibody [NBP3-43954]")
Western Blot: p53 Antibody [NBP3-43954]
Lane 1: Marker [kDa]Lane 2: Human cell line U-251
![p53 Antibody (CL2199) - Azide and BSA Free Immunohistochemistry-Paraffin: p53 Antibody [NBP3-43954]](https://beta-resources.rndsystems.com/images/products/nbp3-43954_mouse-p53-mab-cl2199-azide-and-bsa-free-28220251240556.jpg "Immunohistochemistry-Paraffin: p53 Antibody [NBP3-43954]")
Immunohistochemistry-Paraffin: p53 Antibody [NBP3-43954]
Staining of human colorectal cancer shows strong nuclear positivity in tumor cells, but not in normal mucosa.![p53 Antibody (CL2199) - Azide and BSA Free Immunohistochemistry-Paraffin: p53 Antibody [NBP3-43954]](https://beta-resources.rndsystems.com/images/products/nbp3-43954_mouse-p53-mab-cl2199-azide-and-bsa-free-28220251344251.jpg "Immunohistochemistry-Paraffin: p53 Antibody [NBP3-43954]")
Immunohistochemistry-Paraffin: p53 Antibody [NBP3-43954]
Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.![p53 Antibody (CL2199) - Azide and BSA Free Immunohistochemistry-Paraffin: p53 Antibody [NBP3-43954]](https://beta-resources.rndsystems.com/images/products/nbp3-43954_mouse-p53-mab-cl2199-azide-and-bsa-free-282202513121892.jpg "Immunohistochemistry-Paraffin: p53 Antibody [NBP3-43954]")
Immunohistochemistry-Paraffin: p53 Antibody [NBP3-43954]
Staining of human lung cancer (squamous cell carcinoma) shows strong nuclear positivity in tumor cells.![p53 Antibody (CL2199) - Azide and BSA Free Immunohistochemistry-Paraffin: p53 Antibody [NBP3-43954]](https://beta-resources.rndsystems.com/images/products/nbp3-43954_mouse-p53-mab-cl2199-azide-and-bsa-free-282202513121846.jpg "Immunohistochemistry-Paraffin: p53 Antibody [NBP3-43954]")
Immunohistochemistry-Paraffin: p53 Antibody [NBP3-43954]
Staining of human skin shows moderate to strong nuclear positivity in a subset of epidermal cells.![p53 Antibody (CL2199) - Azide and BSA Free Western Blot: p53 Antibody [NBP3-43954]](https://beta-resources.rndsystems.com/images/products/nbp3-43954_mouse-p53-mab-cl2199-azide-and-bsa-free-282202513341147.jpg "Western Blot: p53 Antibody [NBP3-43954]")
Applications for p53 Antibody (CL2199) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:200 - 1:500
Simple Western
1:20
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.
See Simple Western Antibody Database for Simple Western validation: Tested in Hek293T, separated by Size, antibody dilution of 1:20, apparent MW was 63 kDa
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: p53
Alternate Names
BCC7, LFS1, TP53, TRP53
Gene Symbol
TP53
Additional p53 Products
Product Documents for p53 Antibody (CL2199) - Azide and BSA Free
Product Specific Notices for p53 Antibody (CL2199) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Apoptosis Signaling Pathway
MAPK Signaling: Oxidative Stress Pathway
mTOR Signaling Pathway
