p53 Antibody (CL2199) - BSA Free

Novus Biologicals | Catalog # NBP2-34495

Novus Biologicals

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Knockdown Validated

Cited:

Immunocytochemistry/ Immunofluorescence, PCR

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2199

Format

BSA Free
View all p53 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P04637, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse p53 Antibody (CL2199) - BSA Free (NBP2-34495) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-p53 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for p53 Antibody (CL2199) - BSA Free

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495]

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495]

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.
Western Blot: p53 Antibody (CL2199) [NBP2-34495]

Western Blot: p53 Antibody (CL2199) [NBP2-34495]

Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-P53 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.
Immunocytochemistry/ Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495]

Immunocytochemistry/ Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495]

Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U2OS cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Western Blot: p53 Antibody (CL2199) [NBP2-34495]

Western Blot: p53 Antibody (CL2199) [NBP2-34495]

Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251
Immunocytochemistry/ Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495]

Immunocytochemistry/ Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495]

Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining of human cell line A431 showing cell cycle dependent nuclear (without nucleoli)Staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green
Immunocytochemistry/ Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495]

Immunocytochemistry/ Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495]

Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in MCF7 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495]

Immunocytochemistry/ Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495]

Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U251 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495]

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495]

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells, but not in normal mucosa.
Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495]

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495]

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human lung cancer (squamous cell carcinoma) shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495]

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495]

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skin shows moderate to strong nuclear positivity in a subset of epidermal cells.
Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495]

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495]

Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Simple Western: p53 Antibody (CL2199) [NBP2-34495]

Simple Western: p53 Antibody (CL2199) [NBP2-34495]

Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Simple Western lane view shows a specific band for P53 in 0.2 mg/ml of Hek293T lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: p53 Antibody (CL2199) [NBP2-34495]

Simple Western: p53 Antibody (CL2199) [NBP2-34495]

Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Electropherogram image(s) of corresponding Simple Western lane view. p53 antibody was used at 1:50 dilution on Hek293T lysate(s).

Applications for p53 Antibody (CL2199) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:5000

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:50

Western Blot

1 ug/ml
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Hek293T, separated by Size, antibody dilution of 1:50, apparent MW was 63 kDa.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p53

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

UniProt

P04637

Additional p53 Products

Product Documents for p53 Antibody (CL2199) - BSA Free

Certificate of Analysis

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Product Specific Notices for p53 Antibody (CL2199) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for p53 Antibody (CL2199) - BSA Free

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Protocols

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