p53 Antibody (OTI5E2)

Novus Biologicals | Catalog # NBP2-00723

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Monkey

Cited:

Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5E2
View all p53 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse p53 Antibody (OTI5E2) (NBP2-00723) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and Simple Western. Anti-p53 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for p53 Antibody (OTI5E2)

Western Blot: p53 Antibody (OTI5E2) [NBP2-00723]

Western Blot: p53 Antibody (OTI5E2) [NBP2-00723]

Western Blot: p53 Antibody (5E2) [NBP2-00723] - Analysis of extracts (35ug) from 9 different cell lines by using anti-p53 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: p53 Antibody (OTI5E2) [NBP2-00723]

Immunocytochemistry/ Immunofluorescence: p53 Antibody (OTI5E2) [NBP2-00723]

Immunocytochemistry/Immunofluorescence: p53 Antibody (5E2) [NBP2-00723] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY p53.
Immunohistochemistry-Paraffin: p53 Antibody (OTI5E2) [NBP2-00723]

Immunohistochemistry-Paraffin: p53 Antibody (OTI5E2) [NBP2-00723]

Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-p53 mouse monoclonal antibody.
Flow Cytometry: p53 Antibody (OTI5E2) [NBP2-00723]

Flow Cytometry: p53 Antibody (OTI5E2) [NBP2-00723]

Flow Cytometry: p53 Antibody (5E2) [NBP2-00723] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-p53 antibody, and then analyzed by flow cytometry.
Western Blot: p53 Antibody (OTI5E2) [NBP2-00723]

Western Blot: p53 Antibody (OTI5E2) [NBP2-00723]

Western Blot: p53 Antibody (5E2) [NBP2-00723] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY p53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-p53.
Immunohistochemistry-Paraffin: p53 Antibody (OTI5E2) [NBP2-00723]

Immunohistochemistry-Paraffin: p53 Antibody (OTI5E2) [NBP2-00723]

Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-p53 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: p53 Antibody (OTI5E2) [NBP2-00723]

Immunohistochemistry-Paraffin: p53 Antibody (OTI5E2) [NBP2-00723]

Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-p53 mouse monoclonal antibody.
Flow Cytometry: p53 Antibody (OTI5E2) [NBP2-00723]

Flow Cytometry: p53 Antibody (OTI5E2) [NBP2-00723]

Flow Cytometry: p53 Antibody (5E2) [NBP2-00723] - Analysis of Hela cells, using anti-p53 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Simple Western: p53 Antibody (OTI5E2) [NBP2-00723]

Simple Western: p53 Antibody (OTI5E2) [NBP2-00723]

Simple Western: p53 Antibody (5E2) [NBP2-00723] - Simple Western lane view shows a specific band for p53 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for p53 Antibody (OTI5E2)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Simple Western

1:10

Western Blot

1:500-2000
Application Notes

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Hek293 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:10, apparent MW was 58 kDa.

Reviewed Applications

Read 1 review rated 5 using NBP2-00723 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p53

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq].

Alternate Names

BCC7, LFS1, TP53, TRP53

Entrez Gene IDs

6776 (Human)

Gene Symbol

STAT5A

Additional p53 Products

Product Documents for p53 Antibody (OTI5E2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for p53 Antibody (OTI5E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for p53 Antibody (OTI5E2)

Customer Reviews for p53 Antibody (OTI5E2) (1)

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  • Name: Fugui Li
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human gastric tumor section
    Species: Human
    Verified Customer | Posted 09/24/2014

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