p53 Antibody (OTI5E2) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-73200
![Western Blot: p53 Antibody (OTI5E2)Azide and BSA Free [NBP2-73200]](https://beta-resources.rndsystems.com/images/products/p53-Antibody-OTI5E2-Azide-and-BSA-Free-Western-Blot-NBP2-73200-img0002.jpg "Western Blot: p53 Antibody (OTI5E2)Azide and BSA Free [NBP2-73200]")
Key Product Details
Species Reactivity
Human, Mouse, Monkey
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI5E2
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
Full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
43.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse p53 Antibody (OTI5E2) - Azide and BSA Free (NBP2-00723) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for p53 Antibody (OTI5E2) - Azide and BSA Free
Western Blot: p53 Antibody (OTI5E2)Azide and BSA Free [NBP2-73200]
Western Blot: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY p53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-p53.![Immunocytochemistry/ Immunofluorescence: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]](https://beta-resources.rndsystems.com/images/products/p53-Antibody-OTI5E2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73200-img0005.jpg "Immunocytochemistry/ Immunofluorescence: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]")
Immunocytochemistry/ Immunofluorescence: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]
Immunocytochemistry/Immunofluorescence: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY p53.![Immunohistochemistry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]](https://beta-resources.rndsystems.com/images/products/p53-Antibody-OTI5E2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73200-img0008.jpg "Immunohistochemistry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]")
Immunohistochemistry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]
Immunohistochemistry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-p53 mouse monoclonal antibody.![Flow Cytometry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]](https://beta-resources.rndsystems.com/images/products/p53-Antibody-OTI5E2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73200-img0004.jpg "Flow Cytometry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]")
Flow Cytometry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]
Flow Cytometry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-p53 antibody, and then analyzed by flow cytometry.![Western Blot: p53 Antibody (OTI5E2)Azide and BSA Free [NBP2-73200]](https://beta-resources.rndsystems.com/images/products/p53-Antibody-OTI5E2-Azide-and-BSA-Free-Western-Blot-NBP2-73200-img0001.jpg "Western Blot: p53 Antibody (OTI5E2)Azide and BSA Free [NBP2-73200]")
Western Blot: p53 Antibody (OTI5E2)Azide and BSA Free [NBP2-73200]
Western Blot: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200] - Analysis of extracts (35ug) from 9 different cell lines by using anti-p53 monoclonal antibody.![Immunohistochemistry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]](https://beta-resources.rndsystems.com/images/products/p53-Antibody-OTI5E2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73200-img0006.jpg "Immunohistochemistry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]")
Immunohistochemistry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]
Immunohistochemistry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-p53 mouse monoclonal antibody.![Immunohistochemistry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]](https://beta-resources.rndsystems.com/images/products/p53-Antibody-OTI5E2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73200-img0007.jpg "Immunohistochemistry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]")
Immunohistochemistry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]
Immunohistochemistry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-p53 mouse monoclonal antibody.![Flow Cytometry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]](https://beta-resources.rndsystems.com/images/products/p53-Antibody-OTI5E2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73200-img0003.jpg "Flow Cytometry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]")
Flow Cytometry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]
Flow Cytometry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200] - Analysis of Hela cells, using anti-p53 antibody, (Red), compared to a nonspecific negative control antibody (Blue).![Simple Western: p53 Antibody (OTI5E2)Azide and BSA Free [NBP2-73200]](https://beta-resources.rndsystems.com/images/products/p53-Antibody-OTI5E2-Azide-and-BSA-Free-Simple-Western-NBP2-73200-img0009.jpg "Simple Western: p53 Antibody (OTI5E2)Azide and BSA Free [NBP2-73200]")
Simple Western: p53 Antibody (OTI5E2)Azide and BSA Free [NBP2-73200]
Simple Western: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200] - Simple Western lane view shows a specific band for p53 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Applications for p53 Antibody (OTI5E2) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:500-2000
Application Notes
See Simple Western Antibody Database for Simple Western validation
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: p53
Alternate Names
BCC7, LFS1, TP53, TRP53
Gene Symbol
TP53
Additional p53 Products
Product Documents for p53 Antibody (OTI5E2) - Azide and BSA Free
Product Specific Notices for p53 Antibody (OTI5E2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for p53 Antibody (OTI5E2) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review p53 Antibody (OTI5E2) - Azide and BSA Free and earn rewards!
Have you used p53 Antibody (OTI5E2) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
Apoptosis Signaling Pathway
MAPK Signaling: Oxidative Stress Pathway
mTOR Signaling Pathway
