p53 Antibody (PAb421) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-62555

Recombinant Monoclonal Antibody.
Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey, Rabbit

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vivo assay

Cited:

Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # PAb421

Format

Azide and BSA Free
View all p53 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to aa 371-380 of human p53.

Specificity

Recognises the mammalian mutant and wild-type p53 protein and reacts with an epitope between amino acid residues 370 and 378 (near the C-terminus of the protein), corresponding to the sequence "KKGQSTSRHK".

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Novus Biologicals Rabbit p53 Antibody (PAb421) - Chimeric - Azide and BSA Free (NBP2-62555) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF and IP. Anti-p53 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for p53 Antibody (PAb421) - Chimeric - Azide and BSA Free

Western Blot: p53 Antibody (PAb421)ChimericAzide and BSA Free [NBP2-62555]

Western Blot: p53 Antibody (PAb421)ChimericAzide and BSA Free [NBP2-62555]

Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.
Flow Cytometry: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555]

Flow Cytometry: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555]

Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
p53 Antibody (PAb421) - Chimeric - Azide and BSA Free

Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] -

Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.

Applications for p53 Antibody (PAb421) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: p53

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

Additional p53 Products

Product Documents for p53 Antibody (PAb421) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for p53 Antibody (PAb421) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for p53 Antibody (PAb421) - Chimeric - Azide and BSA Free

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Protocols

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FAQs for p53 Antibody (PAb421) - Chimeric - Azide and BSA Free

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    • Q: I bought p53 Antibody (PAb421). What kind of buffer have to use for dilution? We will use in WB
      A: For use in western blot, you can dilute the antibody in TBST with or without 5% milk
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