Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation, Chromatin Immunoprecipitation Sequencing, Knockdown Validated

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all p53 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residues 50-100 of human tumor protein p53 using the SwissProt entry P04637 (GeneID 7157).

Localization

Cytoplasmic and nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-305, NB100-322, NBP1-19098, NBP1-19097, NB100-376, NB100-1803, NB100-224, NB100-61615, NB100-77315, NB500-160, NB100-381, NB100-381, NB100-596, NB200-347, NB100-97835, NBP1-06527, NBP1-06515

Scientific Data Images for p53 Antibody - BSA Free

Western Blot: p53 Antibody [NB200-171]

Western Blot: p53 Antibody [NB200-171]

Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiP53 antibody used for WB at0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: p53 Antibody [NB200-171]

Immunohistochemistry-Paraffin: p53 Antibody [NB200-171]

Immunohistochemistry-Paraffin: p53 Antibody [NB200-171] - FFPE section of human lung cancer Antibody: Affinity purified rabbit anti- p53 used at 1:5,000 (0.2ug/ml). Detection: DAB
Flow Cytometry: p53 Antibody [NB200-171]

Flow Cytometry: p53 Antibody [NB200-171]

Flow Cytometry: p53 Antibody [NB200-171] - p53 in Jurkat Cells, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 1.5 ug anti-KLH IgG control or anti-p53 NB200-171 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- anti-KLH control IgG; Red- anti-HDMX.
Chromatin Immunoprecipitation Sequencing: p53 Antibody [NB200-171] - Chromatin from MCF7 was immunoprecipitated with anti-p53 antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of p53 binding within a 250 Kb region of chromosome 6 as detected using anti-p53. ChIP-seq validation performed by Active Motif, Carlsbad, CA.
Western Blot: p53 Antibody [NB200-171]

Western Blot: p53 Antibody [NB200-171]

Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (100 ug) from 293T cells that were treated with an siRNA for p53 (+) or mock treated (-). Antibody used at the indicated concentrations.
Flow Cytometry: p53 Antibody [NB200-171]

Flow Cytometry: p53 Antibody [NB200-171]

Flow Cytometry: p53 Antibody [NB200-171] - Baseline p53 expression in human lymphocytes. Image from verified customer review.
Immunoprecipitation: p53 Antibody [NB200-171]

Immunoprecipitation: p53 Antibody [NB200-171]

Immunoprecipitation: p53 Antibody [NB200-171] - Detection of human P53 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-P53 antibody NB200-171 used for IP at 3 ug per reaction. P53 was also immunoprecipitated by rabbit anti-P53 antibody NB100-2204. For blotting immunoprecipitated P53, NB200-171 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for p53 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1.5 ug/10^6 cells in 150 ul

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ChIP-Seq 4 ug/30ug chromatin.

Reviewed Applications

Read 1 review rated 5 using NB200-171 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: p53

The tumor suppressor protein p53 plays an important role in cancer development. The p53 protein is a transcription factor that acts as a checkpoint in the cell cycle, either preventing or initiating programmed cell death. Since cancer occurs as the malfunction in the proliferation of cells, disrupting p53 activity allows abnormal cells to multiply or allows a tumor's progression from benign to malignant.

Alternate Names

BCC7, LFS1, TP53, TRP53

Entrez Gene IDs

7157 (Human)

Gene Symbol

TP53

UniProt

P04637

Additional p53 Products

Product Documents for p53 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for p53 Antibody - BSA Free

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-305, NB100-322, NBP1-19098, NBP1-19097, NB100-376, NB100-1803, NB100-224, NB100-61615, NB100-77315, NB500-160, NB100-381, NB100-381, NB100-596, NB200-347, NB100-97835, NBP1-06527, NBP1-06515

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for p53 Antibody - BSA Free

Customer Reviews for p53 Antibody - BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human PBMC
    Species: Human
    Verified Customer | Posted 06/30/2014
    Baseline p53 expression in human lymphocytes
    p53 Antibody - BSA Free NB200-171

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Protocols

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FAQs for p53 Antibody - BSA Free

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    • Q: I would like to know how you set the lot number of these antibodies. When does the lot number change and what is the difference between lots. Is every lot being QC tested? Or dependent on the host animals (in polyclonal)?
      A: The lot numbers for our products, including NB200-104 and NB200-171, change with every purification or production. So each lot number will refer to the particular purification batch (if the antibody is provided purified) or production batch (if the antibody is not purified). The antibodies are QC tested every lot to ensure specificity and reactivity.
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