p53 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19667

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all p53 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human p53. The exact sequence is proprietary.

Localization

Cytoplasm; Nucleus; Endoplasmic reticulum

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit p53 Antibody - BSA Free (NBP2-19667) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for p53 Antibody - BSA Free

p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.

Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 protein stained by p53 antibody [N1], N-term.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.

Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 protein stained by p53 antibody [N1], N-term.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP2-19667]

Immunocytochemistry/ Immunofluorescence: p53 Antibody [NBP2-19667]

Immunocytochemistry/Immunofluorescence: p53 Antibody [NBP2-19667] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using p53 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

U373 xenograft, using p53 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

U373 xenograft, using p53 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunoprecipitation: p53 Antibody [NBP2-19667]

Immunoprecipitation: p53 Antibody [NBP2-19667]

Immunoprecipitation: p53 Antibody [NBP2-19667] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2. 5 ug of anti-p53 antibody. (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by NBP2-19667. anti-Rabbit IgG Kit was used in the Western blot.
p53 Antibody - BSA Free

Western Blot: p53 Antibody [NBP2-19667] -

Whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term (NBP2-19667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for p53 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP2-19667 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p53

This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq]

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

UniProt

P04637

Additional p53 Products

Product Documents for p53 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for p53 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for p53 Antibody - BSA Free (1)

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  • Name: Shilpa Agarwal
    Application: Western Blot
    Sample Tested: MCF-7 cells
    Species: Human
    Verified Customer | Posted 05/22/2014
    MCF-7 cells treated with vehicle (DMSO) and Doxorubicin (2uM for 24h)
    p53 Antibody - BSA Free NBP2-19667

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Protocols

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