p70 S6 Kinase/S6K Antibody - BSA Free

Name: p70 S6 Kinase/S6K Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP2-23649
Price: 169 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-23649

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Rabbit

Applications

Validated:

Western Blot, Peptide ELISA, ICC/IF (Negative)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free

View all p70 S6 Kinase Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to a C-terminal sequence of the human p70 S6 Kinase protein (between amino acids 350-472). [UniProt P23443-2].

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: canine (87%), equine (81%).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for p70 S6 Kinase/S6K Antibody - BSA Free

Western Blot: p70 S6 Kinase/S6K Antibody [NBP2-23649]

Western Blot: p70 S6 Kinase/S6K Antibody [NBP2-23649] - Western blot Image of anti-p70 S6 Kinase. Whole cell protein from HeLa (lane 1), HEK293 (lane 2) and MCF7 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-p70 S6 Kinase in 1% milk, and detected with an anti-goat HRP secondary antibody using chemiluminescence.

Applications for p70 S6 Kinase/S6K Antibody - BSA Free

Application

Recommended Usage

Peptide ELISA

1:100 - 1:1000

Western Blot

1:100 - 1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Whole sera with 50% Glycerol

Format

BSA Free

Preservative

0.025% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p70 S6 Kinase

S6K encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.

Alternate Names

p70-alpha, RPS6KB1, S6K1, STK14A

Gene Symbol

RPS6KB1

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Product Specific Notices for p70 S6 Kinase/S6K Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.