Pancreatic Polypeptide/PP Antibody

Novus Biologicals | Catalog # NB100-1793

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human, Mouse

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Pancreatic Polypeptide/PP Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TRPRYGKRHKEDT corresponding to internal region according to NP_002713.1.

Reactivity Notes

Monkey and Mouse reactivity reported from verified customer reviews.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Pancreatic Polypeptide/PP Antibody

Immunocytochemistry/ Immunofluorescence: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Immunocytochemistry/ Immunofluorescence: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Pancreatic-Polypeptide-PP-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1793-img0011.jpg
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Pancreas with antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Frozen: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Immunohistochemistry-Frozen: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Immunohistochemistry-Frozen: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Analysis of Pancreatic Polypeptide in mouse adult pancreas tissue. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Adult macaque pancrease paraffin sections following standard protocols. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Pancreas with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Intestine with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Pancreatic Polypeptide/PP Antibody

Immunohistochemistry: Pancreatic Polypeptide/PP Antibody [NB100-1793] -

Immunohistochemistry: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Sorted CD133+ cells originate from pancreatic ducts.(A) Schematic diagram of the experimental procedure. Dissociated pancreatic cells were embedded & cultured as previously described (Lawson et al., 2007). Scale bar, 200 µm. (B) Confocal images of CD133 (green) & CPA1 (red) co-staining in adult human pancreas tissue. Scale bar, 20 µm. (C) CEL expression profiles of FACS-sorted human adult pancreatic cells & isolated islets (islet values normalized to 1). Data are presented as mean ± SEM (n=3). (D) Representative immunostaining pictures of sorted cells. Scale bar, 50 µm.DOI:http://dx.doi.org/10.7554/eLife.00940.005 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24252877), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Pancreatic Polypeptide/PP Antibody

Immunocytochemistry/ Immunofluorescence: Pancreatic Polypeptide/PP Antibody [NB100-1793] -

Immunocytochemistry/ Immunofluorescence: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Histologic comparison of wild type & Seriola dumerili mouse pancreata. Seriola dumerili Ins2 mice (bottom) have normal islet morphology & cyto-architecture compared to littermates with endogenous mouse Ins 1 & Ins 2 (top); insulin (red A–F), glucagon (green; A,D), pancreatic polypeptide (green; B,E), & somatostatin (green; C,F). G, H: Beta cell ultra-structure of NOD & Seriola dumerili Ins2 transgenic. Both NOD (G) & Seriola dumerili Ins 2 transgenic (H) islets contain insulin granules (yellow arrow), though Seriola dumerili Ins2 transgenic insulin granules are lighter in staining intensity compared to the NOD (n = 4; zoom in; inset). (I,J) Insulitis scoring pancreata from 12–15 weeks old NOD & Seriola dumerili Ins2 transgenics (n = 4 per group). Scale bar: 100 um. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30899071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Pancreatic Polypeptide/PP Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Frozen

1:500

Immunohistochemistry-Paraffin

3 ug/ml

Peptide ELISA

Detection limit 1:32000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23221614). Use in immunoblotting reported in scientific literature (PMID: 27572106).

Reviewed Applications

Read 3 reviews rated 5 using NB100-1793 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Pancreatic Polypeptide/PP

Gene ID: 5539 This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.

Alternate Names

INN, Obinepitide, PNP, PP, PPY

Entrez Gene IDs

5539 (Human)

Gene Symbol

PPY

UniProt

P01298, NP_002713.1 (Human)

Additional Pancreatic Polypeptide/PP Products

Product Documents for Pancreatic Polypeptide/PP Antibody

Certificate of Analysis

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Product Specific Notices for Pancreatic Polypeptide/PP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Nutrition and Appetite
Obesity

Citations for Pancreatic Polypeptide/PP Antibody

Customer Reviews for Pancreatic Polypeptide/PP Antibody (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Name: Yves Heremans
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse Pancreas
    Species: Mouse
    Verified Customer | Posted 04/28/2016
    Mouse pancreas stained for PP (green) and insulin (blue)
    Pancreatic Polypeptide/PP Antibody NB100-1793
  • Name: Bryan Tinsley
    Application: Immunohistochemistry-Paraffin
    Verified Customer | Posted 12/18/2013
    Adult macaque pancreas paraffin sections following standard protocols
    Pancreatic Polypeptide/PP Antibody NB100-1793
  • Name: Jonghyeob Lee
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse Adult Pancreas Tissue
    Species: Mouse
    Verified Customer | Posted 08/21/2010
    Pancreatic Polypeptide/PP Antibody NB100-1793

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Protocols

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