PAR1/Thrombin Receptor Antibody (N2-11) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80904

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # N2-11

Format

Azide and BSA Free
View all PAR1 Primary Antibodies »

Product Specifications

Immunogen

The oligopeptide CNATLDPRSFLL from human Thrombin Receptor. [Swiss-Prot# P25116]

Localization

Cell membrane; Multi-pass membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PAR1/Thrombin Receptor Antibody (N2-11) - Azide and BSA Free

Western Blot: PAR1/Thrombin Receptor Antibody (N2-11)Azide and BSA Free [NBP2-80904]

Western Blot: PAR1/Thrombin Receptor Antibody (N2-11)Azide and BSA Free [NBP2-80904]

Western Blot: PAR1/Thrombin Receptor Antibody (N2-11) - Azide and BSA Free [NBP2-80904] - Analysis of Thrombin Receptor expression in human testis using NBP1-71770. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: PAR1/Thrombin Receptor Antibody (N2-11) - Azide and BSA Free [NBP2-80904]

Immunocytochemistry/ Immunofluorescence: PAR1/Thrombin Receptor Antibody (N2-11) - Azide and BSA Free [NBP2-80904]

Immunocytochemistry/Immunofluorescence: PAR1/Thrombin Receptor Antibody (N2-11) - Azide and BSA Free [NBP2-80904] - Thrombin Receptor antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red). Image from the standard format of this antibody.
Immunohistochemistry: PAR1/Thrombin Receptor Antibody (N2-11) - Azide and BSA Free [NBP2-80904]

Immunohistochemistry: PAR1/Thrombin Receptor Antibody (N2-11) - Azide and BSA Free [NBP2-80904]

Immunohistochemistry: PAR1/Thrombin Receptor Antibody (N2-11) - Azide and BSA Free [NBP2-80904] - Analysis of Thrombin Receptor on mouse skin using NBP1-71770. Image from the standard format of this antibody.
Western Blot: PAR1/Thrombin Receptor Antibody (N2-11)Azide and BSA Free [NBP2-80904]

Western Blot: PAR1/Thrombin Receptor Antibody (N2-11)Azide and BSA Free [NBP2-80904]

Western Blot: PAR1/Thrombin Receptor Antibody (N2-11) - Azide and BSA Free [NBP2-80904] - Analysis of PAR1 in whole cell lysate of Neuro2A cell line using 4ug/ml concentration of PAR1 antibody (clone N2-11). The antibody detected a strong band at ~60kDa position and another light band at ~80kDa, both of which appears to be the glycosylated forms of PAR1 protein. Image from the standard format of this antibody.

Applications for PAR1/Thrombin Receptor Antibody (N2-11) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:250 - 1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAR1

Thrombin, the main effector protease of coagulation cascade, is a most potent platelet activator and its actions on platelets are mediated by Thrombin Receptors (also called platelet protease-activated receptors, PARs), which are GPCR members of 7-transmembrane domain receptor superfamily. PARs subtypes includes: PAR-1, PAR-2, PAR-3, and PAR-4, and in addition to platelets, they are also located in smooth muscle cells, endothelial cells, and fibroblasts. PAR1 or thrombin receptor (F2R) is a prototypical GPCR activated by thrombin's lowest concentration compared to other PARs and after cleavage of the extreme N-terminal peptide, the exposed tethered ligand of PAR1 activates itself for participation in diverse physiological processes including coagulation, inflammation, and vascular homeostasis. PAR1 knockout in mouse leads to death of pups in midgestation due to impaired vascular development as well as severe bleeding, whereas endothelial cell-specific reintroduction of PAR1 can rescue the phenotype. PAR1 has emerged as a target for anti-platelet therapies which are useful for ACS (acute coronary syndromes), stable coronary disease, ischemic atherothrombotic stroke, and peripheral arterial disease.

Long Name

Protease-Activated Receptor 1

Alternate Names

Cf2r, F2R, HTR, ThrR, TRGPC

Gene Symbol

F2R

Additional PAR1 Products

Product Documents for PAR1/Thrombin Receptor Antibody (N2-11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PAR1/Thrombin Receptor Antibody (N2-11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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