PARP Antibody

R&D Systems | Catalog # 4338-MC

R&D Systems
Discontinued Product
4338-MC has been discontinued. View all PARP products.

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse, Rat, Bovine

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # C2-10
View all PARP Primary Antibodies »

Product Specifications

Immunogen

Full length calf thymus poly(ADP-ribose) polymerase (PARP)

Specificity

This antibody recognizes the full length (116 kDa) and 85 kDa apoptosis-related and necrosis-related 50kDa, 62 kDa and 74 kDa cleavage fragments of PARP in human, monkey, mouse, rat, bovine and hamster systems. Chicken PARP is not recognized. Other species have not been tested.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for PARP Antibody

Application
Recommended Usage

Immunocytochemistry

1:2000 dilution

Western Blot

1:2000 dilution
Sample: WEHI mouse myelomonocytic leukemia cell line, Jurkat human acute T cell leukemia cell line and CCRF-CEM human acute T lymphoblast leukemia cell line treated with Etoposide

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS with 0.2% BSA and 0.05% sodium azide

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This product is stable as shipped for a least one year when stored at ≤ -20° C. Avoid multiple freeze/thaw cycles by storage in appropriate aliquots. Do not store in a frost-free freezer.

Background: PARP

Poly(ADP-ribose) Polymerase (PARP) is an abundant 116 kDa eukaryotic post-translational modification enzyme. PARP activity is strongly stimulated upon binding to DNA strand breaks and it is thought to be involved in DNA repair, genetic recombination, and apoptosis. PARP is a substrate for certain members of the IL-1 beta converting enzyme (ICE) family of proteases, also known as caspases. During apoptosis, PARP is cleaved into two proteolytic fragments of 25 and 85 kDa.

References

  1. Lamarre, D. et al. 1988, Biochem. Biophys. Acta 950:147.
  2. Lazebnik, Y.A. et al. 1994, Nature 371:346.

Long Name

Poly [ADP-ribose] Polymerase

Alternate Names

ADPRT, PARP1, PPOL

Entrez Gene IDs

142 (Human); 11545 (Mouse)

Gene Symbol

PARP1

Additional PARP Products

Product Documents for PARP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PARP Antibody

For research use only

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Protocols

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FAQs

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