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PARP Antibody

Catalog #: 4338-MC
4 Citations Datasheet / COA / SDS

Discontinued Product

4338-MC has been discontinued.
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Product Details
Citations (4)
FAQs
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

PARP Antibody Summary

Specificity
This antibody recognizes the full length (116 kDa) and 85 kDa apoptosis-related and necrosis-related 50kDa, 62 kDa and 74 kDa cleavage fragments of PARP in human, monkey, mouse, rat, bovine and hamster systems. Chicken PARP is not recognized. Other species have not been tested.
Source
Monoclonal Mouse IgG1 Clone # C2-10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Full length calf thymus poly(ADP-ribose) polymerase (PARP)
Formulation
PBS with 0.2% BSA and 0.05% sodium azide
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:2000 dilution
WEHI mouse myelomonocytic leukemia cell line, Jurkat human acute T cell leukemia cell line and CCRF-CEM human acute T lymphoblast leukemiacell line treated with Etoposide
Immunocytochemistry
1:2000 dilution

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This product is stable as shipped for a least one year when stored at ≤ -20° C. Avoid multiple freeze/thaw cycles by storage in appropriate aliquots. Do not store in a frost-free freezer.

Background: PARP

Poly(ADP-ribose) Polymerase (PARP) is an abundant 116 kDa eukaryotic post-translational modification enzyme. PARP activity is strongly stimulated upon binding to DNA strand breaks and it is thought to be involved in DNA repair, genetic recombination, and apoptosis. PARP is a substrate for certain members of the IL-1 beta converting enzyme (ICE) family of proteases, also known as caspases. During apoptosis, PARP is cleaved into two proteolytic fragments of 25 and 85 kDa.

References
  1. Lamarre, D. et al. 1988, Biochem. Biophys. Acta 950:147.
  2. Lazebnik, Y.A. et al. 1994, Nature 371:346.
Long Name
Poly [ADP-ribose] Polymerase
Entrez Gene IDs
142 (Human); 11545 (Mouse)
Alternate Names
ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADPRT 1; ADPRT; ADPRTADP-ribosyltransferase NAD(+); EC 2.4.2; EC 2.4.2.30; NAD(+) ADP-ribosyltransferase 1; PARP apoptosis; PARP; PARP1; PARP-1; PARPADPRT1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL; PPOLpADPRT-1

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Citations for PARP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Calreticulin is a Critical Cell Survival Factor in Malignant Neoplasms
    Authors: A Han, C Li, T Zahed, M Wong, I Smith, K Hoedel, D Green, AD Boiko
    PLoS Biol., 2019-09-30;17(9):e3000402.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Non-genotoxic MDM2 inhibition selectively induces pro-apoptotic p53 gene signature in chronic lymphocytic leukemia cells
    Authors: C Ciardullo, E Aptullahog, L Woodhouse, WY Lin, JP Wallis, H Marr, S Marshall, N Bown, E Willmore, J Lunec
    Haematologica, 2019-04-19;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Gingipains from Porphyromonas gingivalis W83 synergistically disrupt endothelial cell adhesion and can induce caspase-independent apoptosis.
    Authors: Sheets SM, Potempa J, Travis J, Fletcher HM, Casiano CA
    Infect. Immun., 2006-10-01;74(10):5667-78.
    Species: Bovine
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Type I collagen promotes the malignant phenotype of pancreatic ductal adenocarcinoma.
    Authors: Armstrong T, Murphy LB, Bateman AC, Conti JA, Fine DR, Johnson CD, Benyon RC, Iredale JP
    Clin. Cancer Res., 2004-11-01;10(21):7427-37.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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