Pax2 Antibody (CL9469)

Novus Biologicals | Catalog # NBP3-52098

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL9469
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Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from Q02962, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Pax2 Antibody (CL9469)

Pax2 Antibody (CL9469) Immunohistochemistry: Pax2 Antibody (CL9469) [NBP3-52098]

Immunohistochemistry: Pax2 Antibody (CL9469) [NBP3-52098]

Staining of human endometrium shows strong nuclear positivity in glandular cells.
Pax2 Antibody (CL9469) Immunohistochemistry: Pax2 Antibody (CL9469) [NBP3-52098]

Immunohistochemistry: Pax2 Antibody (CL9469) [NBP3-52098]

Analysis in human kidney and prostate tissues. Corresponding Pax2 RNA-seq data are presented for the same tissues.
Pax2 Antibody (CL9469) Immunohistochemistry: Pax2 Antibody (CL9469) [NBP3-52098]

Immunohistochemistry: Pax2 Antibody (CL9469) [NBP3-52098]

Staining of human kidney shows strong nuclear positivity in cells in distal tubules.
Pax2 Antibody (CL9469) Immunohistochemistry: Pax2 Antibody (CL9469) [NBP3-52098]

Immunohistochemistry: Pax2 Antibody (CL9469) [NBP3-52098]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Pax2 Antibody (CL9469) Western Blot: Pax2 Antibody (CL9469) [NBP3-52098]

Western Blot: Pax2 Antibody (CL9469) [NBP3-52098]

Analysis in human cell line EFO-21.
Pax2 Antibody (CL9469) Immunohistochemistry: Pax2 Antibody (CL9469) [NBP3-52098]

Immunohistochemistry: Pax2 Antibody (CL9469) [NBP3-52098]

Staining of human renal cancer shows moderate nuclear positivity in tumor cells.
Pax2 Antibody (CL9469) Immunocytochemistry/ Immunofluorescence: Pax2 Antibody (CL9469) [NBP3-52098]

Immunocytochemistry/ Immunofluorescence: Pax2 Antibody (CL9469) [NBP3-52098]

Staining of EFO-21 cells showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Pax2 Antibody (CL9469) Immunohistochemistry: Pax2 Antibody (CL9469) [NBP3-52098]

Immunohistochemistry: Pax2 Antibody (CL9469) [NBP3-52098]

Staining of human prostate shows no positivity in glandular cells as expected.

Applications for Pax2 Antibody (CL9469)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pax2

PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor supressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants.

Long Name

Paired Box Gene 2

Alternate Names

paired box 2, paired box gene 2, paired box homeotic gene 2, paired box protein Pax-2

Gene Symbol

PAX2

Additional Pax2 Products

Product Documents for Pax2 Antibody (CL9469)

Certificate of Analysis

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Product Specific Notices for Pax2 Antibody (CL9469)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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