Pax6 Antibody

Novus Biologicals | Catalog # NB300-750

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Fish, Monkey, Primate, Sheep

Cited:

Human, Mouse, Fish

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Pax6 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues C R(267) E E K L R N Q R R Q A S N T P S H I(285) of mouse PAX6.

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 29888788).

Localization

Nuclear

Marker

Neural Marker

Specificity

Detects a ~47 kDa protein representing PAX6 (Schwann Cell Marker) from rat whole eye extracts and ovine retinal extracts.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Pax6 Antibody

Western Blot: Pax6 Antibody [NB300-750]

Western Blot: Pax6 Antibody [NB300-750]

Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract
Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NB300-750]

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NB300-750]

Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - ARPE-19 monolayer on glass substrate. Fixative Methanol Blocking Goat Serum. Dilution 1:100. Incubation 1h @ RT. This image was submitted via customer Review.
Western Blot: Pax6 Antibody [NB300-750]

Western Blot: Pax6 Antibody [NB300-750]

Western Blot: Pax6 Antibody [NB300-750] - Analysis of rat whole eye extract
Western Blot: Pax6 Antibody [NB300-750]

Western Blot: Pax6 Antibody [NB300-750]

Western Blot: Pax6 Antibody [NB300-750] - Analysis of various human, mouse and non-human primate whole cell lysates.
Western Blot: Pax6 Antibody [NB300-750]

Western Blot: Pax6 Antibody [NB300-750]

Western Blot: Pax6 Antibody [NB300-750] - Analysis of extracts from HeLa, U251 and mouse brain using PAX6 antibody (NB300-750, 1:100).
Western Blot: Pax6 Antibody [NB300-750]

Western Blot: Pax6 Antibody [NB300-750]

Western Blot: Pax6 Antibody [NB300-750] - Analysis of Pax6 in skate (Leucoraja erinacea) brain lysate using anti-Pax6 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NB300-750]

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NB300-750]

Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX6 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin were stained with Hoechst 33342 dye.
Immunoprecipitation: Pax6 Antibody [NB300-750]

Immunoprecipitation: Pax6 Antibody [NB300-750]

Immunoprecipitation: Pax6 Antibody [NB300-750] - Analysis of PAX6 was performed on 293T cells. Antigen: antibody complexes were formed by incubating 500ug whole cell lysate with 5ug of PAX6 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a PAX6 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:1000 for at least one hour.
Pax6 Antibody

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NB300-750] -

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NB300-750] - Stepwise differentiation of human iPSCs towards renal progenitor cells (RPCs).(a) Schematic description of the two-stage protocol applied to iPSC-renal commitment. (b, c, d, e) Immunofluorescence of iPSCs (derived from retroviral transfected dermal fibroblasts) exposed to differentiating media. (b) The pluripotency markers SSEA4, TRA-1-81, Nanog, ME marker T(Bry) & IM marker Osr1. (c) IM & MM marker expression as Wt1, Pax8, Pax2, Six2 & Sall1. (d) Renal progenitor markers CD133, CD24, NCAM, glomerular epithelial marker Claudin1 & proximal tubular epithelial markers AQP1, GGT1. (e) Markers identifying endodermal AFP, ectodermal Pax6, & cardiac mesodermal Nkx2.5 derivation. Nuclei are stained with DAPI (blue). Scale bars: 20 μm (b, c, d, e). Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep08826), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Pax6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunoprecipitation

5 ug

Western Blot

1:1000
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID: 22837015).

Reviewed Applications

Read 4 reviews rated 4.5 using NB300-750 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Pax6

PAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters' anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.

Long Name

Paired Box Gene 6

Alternate Names

keratitis), MGC17209, Oculorhombin, paired box 6, paired box protein Pax-6

Entrez Gene IDs

5080 (Human); 18508 (Mouse); 25509 (Rat)

Gene Symbol

PAX6

UniProt

P63016 (Rat)

Additional Pax6 Products

Product Documents for Pax6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Pax6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Pax6 Antibody (4)

4.5 out of 5
4 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Pax6 Antibody
    Name: Fabio Marongiu
    Application: Immunocytochemistry
    Sample Tested: ARPE-19 cells
    Species: Human
    Verified Customer | Posted 08/29/2017
    ARPE-19 monolayer on glass substrate
    Fixative Methanol Blocking Goat Serum Dilution 1:100 Incubation 1h @ RT
    Pax6 Antibody NB300-750
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Hindbrain
    Species: Other
    Verified Customer | Posted 04/20/2016
    Elasmobranch hindbrain- Pax6 in green
    Pax6 Antibody NB300-750
  • Name: Christos Suriano
    Application: Western Blot
    Sample Tested: Skate brain
    Species: Other
    Verified Customer | Posted 02/09/2016
    Pax6 in basal vertebrate
    Pax6 Antibody NB300-750
  • Name: Bryan Tinsley
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 06/13/2014
    Western blot analysis of extracts from HeLa, U251 and mouse brain using PAX6 antibody (NB300-750, 1:100).
    Pax6 Antibody NB300-750

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Pax6 Antibody

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I am just wondering if this specific epitope is conserved from rabbits to lampreys and skates

    A: Yes, the immunogen is pretty conserved and is shared with the sequence available at Uniprot site so it may work in your mentioned species.

  • Q: What PAX6 antibody is recommend to use in ICC staining of human stem cells differentiated neural precursors?

    A:

    By performing a search on PAX6 then refining this to antibodies which have been validated with human samples and for ICC, I can see that we have three products to meet your requirements. You can see all three of these antibodies. These products are listed based on sales and citations, however all of our antibodies are covered by our 100% guarantee to work in the species and applications listed on our website and on our product datasheets. You can read more about our quality guarantee here.

  • Q: I am just wondering if this specific epitope is conserved from rabbits to lampreys and skates

    A: Yes, the immunogen is pretty conserved and is shared with the sequence available at Uniprot site so it may work in your mentioned species.

  • Q: What PAX6 antibody is recommend to use in ICC staining of human stem cells differentiated neural precursors?

    A:

    By performing a search on PAX6 then refining this to antibodies which have been validated with human samples and for ICC, I can see that we have three products to meet your requirements. You can see all three of these antibodies. These products are listed based on sales and citations, however all of our antibodies are covered by our 100% guarantee to work in the species and applications listed on our website and on our product datasheets. You can read more about our quality guarantee here.

Showing  1 - 2 of 2 FAQs Showing All
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