Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # OMNI379

Format

Azide and BSA Free
View all PBEF/Visfatin Primary Antibodies »

Product Specifications

Immunogen

Recombinant human PBEF/Visfatin/NAMPT (visfatin/PBEF).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PBEF/Visfatin/NAMPT Antibody (OMNI379) - Azide and BSA Free

Western Blot: PBEF/Visfatin/NAMPT Antibody (OMNI379)Azide and BSA Free [NBP2-80036]

Western Blot: PBEF/Visfatin/NAMPT Antibody (OMNI379)Azide and BSA Free [NBP2-80036]

Western Blot: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1:2000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.
Immunocytochemistry/ Immunofluorescence: PBEF/Visfatin/NAMPT Antibody (OMNI379) - Azide and BSA Free [NBP2-80036]

Immunocytochemistry/ Immunofluorescence: PBEF/Visfatin/NAMPT Antibody (OMNI379) - Azide and BSA Free [NBP2-80036]

Immunocytochemistry/Immunofluorescence: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunofluorescent confocal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Nampt (Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).
Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody (OMNI379) - Azide and BSA Free [NBP2-80036]

Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody (OMNI379) - Azide and BSA Free [NBP2-80036]

Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunohistochemical staining of Nampt using anti-Nampt (OMNI379) in human kidney tissue (1:500 dilution). Immunoperoxidase staining of formalin-fixed and paraffin-embedded (FFPE) human kidney showing cytoplasmic staining (200x, brown color).
Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody (OMNI379) - Azide and BSA Free [NBP2-80036]

Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody (OMNI379) - Azide and BSA Free [NBP2-80036]

Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunohistochemical staining of Nampt using anti-Nampt (Visfatin/PBEF), mAb (OMNI379) in formalin-fixed and paraffin-embedded (FFPE) human colon tissue (15 ug/mL).
PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1:2000 dilution. 1. Human Nampt (Visfatin/PBEF) (His-tagged). 2. Human Nampt (Visfatin/PBEF) (FLAG(R)-tagged). 3. Mouse Nampt (Visfatin/PBEF) (His-tagged). 4. Rat Nampt (Visfatin/PBEF) (His-tagged). 5. Human Nampt (Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.
Immunoprecipitation: PBEF/Visfatin/NAMPT Antibody (OMNI379) - Azide and BSA Free [NBP2-80036]

Immunoprecipitation: PBEF/Visfatin/NAMPT Antibody (OMNI379) - Azide and BSA Free [NBP2-80036]

Immunoprecipitation: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted and visualized by western blot with anti-human Nampt (Visfatin/PBEF) pAb.

Applications for PBEF/Visfatin/NAMPT Antibody (OMNI379) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:2000 - 1:10000

Flow Cytometry

1:1000

Immunocytochemistry/ Immunofluorescence

1:500 - 1:1000

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:2000 - 1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um-filtered solution in PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PBEF/Visfatin

NAMPT encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10.

Long Name

Pre-B-Cell Colony-Enhancing Factor 1

Alternate Names

NAmPRTase, NAMPT, NMPRTase, PBEF1, Visfatin

Gene Symbol

NAMPT

Additional PBEF/Visfatin Products

Product Documents for PBEF/Visfatin/NAMPT Antibody (OMNI379) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PBEF/Visfatin/NAMPT Antibody (OMNI379) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PBEF/Visfatin/NAMPT Antibody (OMNI379) - Azide and BSA Free

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Protocols

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FAQs

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Associated Pathways

Adipokines & Insulin Signaling Pathways Adipokines & Insulin Signaling Pathway Thumbnail