PCNA Antibody (PC10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80905

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Chicken, Drosophila, Fish, Marsupial, Primate, Rabbit, Yeast, Zebrafish

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # PC10

Format

Azide and BSA Free
View all PCNA Primary Antibodies »

Product Specifications

Immunogen

Protein A-rat PCNA fusion obtained from pC2T

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 30075024).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PCNA Antibody (PC10) - Azide and BSA Free

Western Blot: PCNA Antibody (PC10)Azide and BSA Free [NBP2-80905]

Western Blot: PCNA Antibody (PC10)Azide and BSA Free [NBP2-80905]

Western Blot: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905]

Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905]

Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/mL overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905]

Immunohistochemistry: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905]

Immunohistochemistry: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Analysis of paraffin-embedded human colon carcinoma, showing nuclear localization. Image from the standard format of this antibody.
Flow Cytometry: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905]

Flow Cytometry: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905]

Flow Cytometry: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - An intracellular stain was performed on HepG2 cells with PCNA Antibody (PC10) NB500-106G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to DyLight 488.
Western Blot: PCNA Antibody (PC10)Azide and BSA Free [NBP2-80905]

Western Blot: PCNA Antibody (PC10)Azide and BSA Free [NBP2-80905]

Western Blot: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Analysis of human (HeLa lysate), murine (SV-T2 lysate), bovine (BAEC lysate), and porcine (PAE lysate) cell extracts. Image from the standard format of this antibody.
Western Blot: PCNA Antibody (PC10)Azide and BSA Free [NBP2-80905]

Western Blot: PCNA Antibody (PC10)Azide and BSA Free [NBP2-80905]

Western Blot: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - HeLa cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Image from the standard format of this antibody.
Immunohistochemistry: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905]

Immunohistochemistry: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905]

Immunohistochemistry: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905]

Immunohistochemistry-Paraffin: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905]

Immunohistochemistry-Paraffin: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Paraformaldehyde fixed frozen section of brain from murine embryo using PCNA antibody clone PC10 (green), an IB4 antibody (red) and DAPI. Image provided by Dr. Siegenthaler via product review. Image from the standard format of this antibody.
Simple Western: PCNA Antibody (PC10)Azide and BSA Free [NBP2-80905]

Simple Western: PCNA Antibody (PC10)Azide and BSA Free [NBP2-80905]

Simple Western: PCNA Antibody (PC10) - Azide and BSA Free [NBP2-80905] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.

Applications for PCNA Antibody (PC10) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

1:10 - 1:500. Use reported in scientific literature (PMID 26883631)

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

ELISA

reported in scientific literature

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:2000. Use reported in scientific literature (PMID 22068968)

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry Free-Floating

reported in scientific literature (PMID 27212918)

Immunohistochemistry-Frozen

1:10 - 1:500

Immunoprecipitation

1:100

Simple Western

1:200

Western Blot

1:2000
Application Notes

It detects a band at 30 kDa by Western blot. For IHC on paraffin sections, heat-mediated citrate buffer antigen retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:200. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Tris-Glycine, 0.15M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCNA

PCNA (Proliferating Cell Nuclear Antigen), plays a central role in coordinating the association of replication factors during DNA replication as well as during recognition and repair of DNA damage. PCNA forms a toroidal, ring-shaped structure of 90 kD by the symmetric association of three identical monomers. The ring encircles the DNA, acts as a platform upon which polymerases and other proteins dock to perform various DNA metabolic processes, and functions as a DNA polymerase-delta co-factor. The association of proteins with the replication fork through interactions with PCNA is regulated by several mechanisms. PCNA interacts with numerous proteins to facilitate DNA replication and repair, epigenetic processes and sister chromatid cohesion. PCNA has its highest expression during G1and S-phases, and its expression decreases in G2 and M-phases. This marker is also present in the early G0 phase because of its long half-live of 8-20 h. PCNA is a nuclear nonhistone protein that is necessary for DNA synthesis and is an accessory protein for DNA polymerase alpha, which is elevated during the G1/S phase of the cell cycle. Several studies have been performed to evaluate cell proliferation using PCNA and Ki-67 in different tumors of various origins; compared with PCNA, Ki-67 has been shown to be more sensitive and specific in the various tumors.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Gene Symbol

PCNA

Additional PCNA Products

Product Documents for PCNA Antibody (PC10) - Azide and BSA Free

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Product Specific Notices for PCNA Antibody (PC10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PCNA Antibody (PC10) - Azide and BSA Free

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Protocols

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