PCNA Antibody (PC10) - BSA Free

Novus Biologicals | Catalog # NB500-106

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Chicken, Drosophila, Fish, Marsupial, Primate, Rabbit, Yeast, Zebrafish

Cited:

Human, Mouse, Rat, Porcine, Avian - Chicken, Fish, Fish - Danio rerio (Zebrafish), Marsupial, Primate, Yeast

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # PC10

Format

BSA Free
View all PCNA Primary Antibodies »

Product Specifications

Immunogen

Protein A-rat PCNA fusion obtained from pC2T

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33802807). Use in Marsupial reported in scientific literature (PMID:29253253).

Marker

Proliferation Marker

Specificity

Specific for PCNA p36 protein expressed at high levels in proliferating cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PCNA Antibody (PC10) - BSA Free

Simple Western: PCNA Antibody (PC10) [NB500-106]

Simple Western: PCNA Antibody (PC10) [NB500-106]

Simple Western: PCNA Antibody (PC10) [NB500-106] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106]

Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106]

Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - NIH3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA Antibody (PC10) NB500-106 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: PCNA Antibody (PC10) [NB500-106]

Immunohistochemistry-Paraffin: PCNA Antibody (PC10) [NB500-106]

Immunohistochemistry-Paraffin: PCNA Antibody (PC10) [NB500-106] - Analysis of paraffin-embedded human colon carcinoma, showing nuclear localization.
Western Blot: PCNA Antibody (PC10) [NB500-106]

Western Blot: PCNA Antibody (PC10) [NB500-106]

Western Blot: PCNA Antibody (PC10) [NB500-106] - Analysis of human (HeLa lysate), murine (SV-T2 lysate), bovine (BAEC lysate), and porcine (PAE lysate) cell extracts.
Western Blot: PCNA Antibody (PC10) [NB500-106]

Western Blot: PCNA Antibody (PC10) [NB500-106]

Western Blot: PCNA Antibody (PC10) [NB500-106] - Western blot of PCNA levels across different stages of the cell cycle. U-2 OS cells were synchronised at G1/S with 2 mM thymidine an released for 10 hours for G2/M or re-arrested in G2 for extended duration with 10uM RO-3306. Antibody at 1:2000. WB image submitted by a verified customer review.
Immunohistochemistry-Frozen: PCNA Antibody (PC10) [NB500-106]

Immunohistochemistry-Frozen: PCNA Antibody (PC10) [NB500-106]

Immunohistochemistry-Frozen: PCNA Antibody (PC10) [NB500-106] - Paraformaldehyde fixed frozen section of brain from murine embryo using PCNA antibody clone PC10 (green), an IB4 antibody (red) and DAPI. Image provided by Dr. Siegenthaler via product review.
Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106]

Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106]

Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - PC12 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA Antibody (PC10) NB500-106 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106]

Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106]

Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/mL overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: PCNA Antibody (PC10) [NB500-106]

Immunohistochemistry: PCNA Antibody (PC10) [NB500-106]

PCNA-Antibody-PC10-Immunohistochemistry-NB500-106-img0013.jpg
Flow Cytometry: PCNA Antibody (PC10) [NB500-106]

Flow Cytometry: PCNA Antibody (PC10) [NB500-106]

Flow Cytometry: PCNA Antibody (PC10) [NB500-106] - An intracellular stain was performed on HepG2 cells with PCNA Antibody (PC10) NB500-106G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.
Western Blot: PCNA Antibody (PC10) [NB500-106]

Western Blot: PCNA Antibody (PC10) [NB500-106]

Western Blot: PCNA Antibody (PC10) [NB500-106] - HeLa cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Western Blot: PCNA Antibody (PC10) [NB500-106]

Western Blot: PCNA Antibody (PC10) [NB500-106]

Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry: PCNA Antibody (PC10) [NB500-106]

Immunohistochemistry: PCNA Antibody (PC10) [NB500-106]

Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.
PCNA Antibody (PC10)

Chromatin Immunoprecipitation: PCNA Antibody (PC10) [NB500-106] -

Chromatin Immunoprecipitation: PCNA Antibody (PC10) [NB500-106] - Events taking place during a transient telomere dysfunction. All strains were cdc13–1, incubated for 160 min at 36°C to induce telomere uncapping, followed by 90 min at 23°C to allow telomere re-capping. Nocodazole & BrdU were added to the cultures at time 0. (A) Dynamics of ssDNA loss in sub-telomeres. (B) BrdU incorporation in sub-telomeres, minus incorporation at ‘CEN’ (e.g. a centromere-proximal locus, in this case ERG26). (C–F) Dynamics of the protein association with sub-telomeres, measured for Rap1 & major DNA synthesis factors (indicated above each graph). ChIP (%) was calculated as the fraction of immunoprecipitated sub-telomeric DNA, minus the fraction precipitated at ‘CEN’ (G) Dynamics of ssDNA loss at YER188W. (H) BrdU incorporation at YER188W, minus incorporation at ‘CEN’. (I–L) The protein association with YER188W was analyzed as in C–F. (M) ssDNA at ‘CEN’. (N) BrdU incorporation at ‘CEN’. (O–R) Association of proteins with ‘CEN’. (S) Growth of serial dilution of wild-type (first row) & cdc13–1 cells with or without additional mutations (indicated on the left of each row) at temperatures indicated above each plate. The plate shown on the right was cycled three times between 4 h at 36°C (to accumulate ssDNA) & 4 h at 21°C (to re-synthesize DNA), followed by incubation at 21°C for another two days. Image collected & cropped by CiteAb from the following publication (https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PCNA Antibody (PC10)

Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106] -

Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - Development of the germinal zones in the tree shrew neocortex. (A–D) Immunofluorescence for PCNA (yellow) & DAPI staining (blue) on 30 μm-cryosections of E32–P1 tree shrew neocortex. The top margin of the image corresponds to the transition zone SVZ/intermediate zone. Scale bars, 50 μm. VZ, ventricular zone; SVZ, subventricular zone; iSVZ, inner SVZ; oSVZ, outer SVZ. (E) Quantification of the VZ thickness of the E32–P1 tree shrew neocortex. (F) Quantification of the SVZ thickness of the E32–P1 tree shrew neocortex. (G) Quantification of the VZ & SVZ thickness of the E32–P1 tree shrew neocortex, expressed as percentage of the sum of VZ & SVZ. (H) Quantification of the iSVZ & oSVZ thickness of the E37 tree shrew neocortex, expressed as percentage of the sum of iSVZ & oSVZ. (E–H) Data represent mean ± SD & were obtained from two consecutive sections of two brains each. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29725291), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PCNA Antibody (PC10) - BSA Free

Western Blot: PCNA Antibody (PC10) - BSA Free [NB500-106] -

Nuclear accumulation of SIRT4 is increased in renal tubules after injury.(A) Western blots analysis of SIRT4, FN1, COL1A1, and Tubulin in the kidney of UUO, uIRI, and sham mice (control group). (B) Nuclear fractions were prepared from the kidney of UUO, uIRI, and sham mice. Nuclear PCNA and cytoplasmic tubulin were used as controls. (C, D) Representative images of immunohistochemical staining of SIRT4 (scale bar, 50 μm) in the kidneys from mice that underwent sham surgery, UUO surgery on day 10 post surgery or uIRI surgery on day 28 post surgery. (E, F) Representative images of Masson’s trichrome staining (the upper panel; scale bar = 50 μm) and SIRT4 immumohistochemical staining (the bottom panel; scale bar = 20 μm) in the kidney sections from patients with CKD (n=8) and minimal change disease (control group, n=1).Figure 1—source data 1.Original files for western blot analysis displayed in Figure 1A, B.Figure 1—source data 2.The uncropped gels or blots with the relevant bands clearly labeled in Figure 1A and B.Original files for western blot analysis displayed in Figure 1A, B.The uncropped gels or blots with the relevant bands clearly labeled in Figure 1A and B. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/98524), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PCNA Antibody (PC10) - BSA Free

Western Blot: PCNA Antibody (PC10) - BSA Free [NB500-106] -

U0126 prevents SIRT4 overexpression induced kidney fibrosis in UUO mice.(A, B) AAV9-Ksp-Sirt4 was injected into kidneys of mice in situ at three independent points in situ. After 2-week transfection, the mice received UUO surgery, and then mice were treated with U0126 (10 mg/kg body weight) or vehicle for 10 days (n=6 per group). (A) Organelle separation experiment and immunoblot analysis detected the localization of SIRT4 and Tubulin in the kidneys of mice. (B) Representative images of immunohistochemical staining of SIRT4 (scale bar, 50 μm) in the kidney sections of mice.Figure 8—figure supplement 1—source data 1.Original files for western blot analysis displayed in Figure 8—figure supplement 1A.Figure 8—figure supplement 1—source data 2.The uncropped gels or blots with the relevant bands clearly labeled in Figure 8—figure supplement 1A.Original files for western blot analysis displayed in Figure 8—figure supplement 1A.The uncropped gels or blots with the relevant bands clearly labeled in Figure 8—figure supplement 1A. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/98524), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PCNA Antibody (PC10) - BSA Free

Western Blot: PCNA Antibody (PC10) - BSA Free [NB500-106] -

Inhibition of nucleus accumulation of beta -catenin failed to suppresses kidney fibrosis induced by SIRT4 overexpression in UUO mice.(A–C) AAV9-Ksp-Sirt4 or AAV-Ctrl was injected into kidneys of mice in situ at three independent points in situ. After 2-week transfection, the mice received UUO surgery. After UUO surgery, mice were treated with vehicle or MSAB for 10 days (n=6 per group). (A) Western blot analysis of the expression of SIRT4, CCN2, FN1, COL3A1, alpha -SMA, and Tubulin in the kidneys from mice. (B) Representative images of Masson’s trichrome staining and Sirius red staining in kidney sections of mice (scale bar, 100 μm). (C) The mRNA level of Col1a1, Fn1, Eln, Ccn2, Acta2, and Col3a1 in the kidney of mice. For all panels, data are presented as mean +/- SD. ns: not significant difference, *p<0.05, **p<0.01, ***p<0.001 by one-way ANOVA with Bonferroni correction test.Figure 8—figure supplement 3—source data 1.Original files for western blot analysis displayed in Figure 8—figure supplement 3A.Figure 8—figure supplement 3—source data 2.The uncropped gels or blots with the relevant bands clearly labeled in Figure 8—figure supplement 3A.Original files for western blot analysis displayed in Figure 8—figure supplement 3A.The uncropped gels or blots with the relevant bands clearly labeled in Figure 8—figure supplement 3A. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/98524), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PCNA Antibody (PC10) - BSA Free

Western Blot: PCNA Antibody (PC10) - BSA Free [NB500-106] -

Exosomes contain anti-SIRT4 antibody alleviated UUO-induced kidney fibrosis.(A–D) After sham or UUO surgery, WT mice were treated with exosomes null or contain anti-SIRT4 for 10 days (n=6 per group). (A) Western blot analysis of the expression of CCN2, FN1, COL1A1, COL3A1, E-cadherin. alpha -SMA and Tubulin in the kidney from mice. (B) Representative images of Masson’s trichrome staining and Sirius red staining in kidneys sections of mice (scale bar, 100 μm). (C) The mRNA level of Col1a1, Fn1, Eln, Ccn2, Acta2, and Col3a1 in the kidney of mice. (D) Organelle separation experiment and immunoblot analysis detected the localization of SIRT4 in the kidneys of mice. For all panels, data are presented as mean +/- SD. *p<0.05, **p<0.01, ***p<0.001 by one-way ANOVA with Bonferroni correction test.Figure 8—figure supplement 2—source data 1.Original files for western blot analysis displayed in Figure 8—figure supplement 2A, D.Figure 8—figure supplement 2—source data 2.The uncropped gels or blots with the relevant bands clearly labeled in Figure 8—figure supplement 2A, D.Original files for western blot analysis displayed in Figure 8—figure supplement 2A, D.The uncropped gels or blots with the relevant bands clearly labeled in Figure 8—figure supplement 2A, D. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/98524), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PCNA Antibody (PC10) - BSA Free

Western Blot: PCNA Antibody (PC10) - BSA Free [NB500-106] -

(a) Western blot analysis of PARP1 and PCNA protein levels. (b) Representative images of immunoreactive bands. Data are presented as means +/- SEM of densitometric analysis of immunoreactive bands normalized to internal reference protein (GAPDH). One-way ANOVA p < 0.05; a,bp < 0.05, Holm–Sidak post hoc multiple comparison. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33802807), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PCNA Antibody (PC10) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

1:10 - 1:500. Use reported in scientific literature (PMID 26883631)

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

ELISA

reported in scientific literature

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:1000-1:2000. Use reported in scientific literature (PMID 22068968)

Immunohistochemistry

1:10-1:500

Immunohistochemistry Free-Floating

reported in scientific literature (PMID 27212918)

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:500-1:1000

Immunoprecipitation

1:100

Simple Western

1:200

Western Blot

1:2000
Application Notes

It detects a band at 30 kDa by Western blot. For IHC on paraffin sections, heat-mediated citrate buffer antigen retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:200, apparent MW was 36 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 5 reviews rated 4.8 using NB500-106 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCNA

PCNA (Proliferating Cell Nuclear Antigen), plays a central role in coordinating the association of replication factors during DNA replication as well as during recognition and repair of DNA damage. PCNA forms a toroidal, ring-shaped structure of 90 kD by the symmetric association of three identical monomers. The ring encircles the DNA, acts as a platform upon which polymerases and other proteins dock to perform various DNA metabolic processes, and functions as a DNA polymerase-delta co-factor. The association of proteins with the replication fork through interactions with PCNA is regulated by several mechanisms. PCNA interacts with numerous proteins to facilitate DNA replication and repair, epigenetic processes and sister chromatid cohesion. PCNA has its highest expression during G1and S-phases, and its expression decreases in G2 and M-phases. This marker is also present in the early G0 phase because of its long half-live of 8-20 h. PCNA is a nuclear nonhistone protein that is necessary for DNA synthesis and is an accessory protein for DNA polymerase alpha, which is elevated during the G1/S phase of the cell cycle. Several studies have been performed to evaluate cell proliferation using PCNA and Ki-67 in different tumors of various origins; compared with PCNA, Ki-67 has been shown to be more sensitive and specific in the various tumors.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Entrez Gene IDs

5111 (Human); 18538 (Mouse); 25737 (Rat)

Gene Symbol

PCNA

UniProt

P12004 (Human)

Additional PCNA Products

Product Documents for PCNA Antibody (PC10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PCNA Antibody (PC10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PCNA Antibody (PC10) - BSA Free

Customer Reviews for PCNA Antibody (PC10) - BSA Free (5)

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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • PCNA Antibody (PC10)
    Name: Robert Lu
    Application: Western Blot
    Sample Tested: U-2 OS cells
    Species: Human
    Verified Customer | Posted 12/17/2020
    Western blot of PCNA levels across different stages of the cell cycle. U-2 OS cells were synchronised at G1/S with 2mM thymidine an released for 10 hours for G2/M or re-arrested in G2 for extended duration with 10uM RO-3306.
    Used at 1:2000 dilution in 0.5% milk PBST. Incubated overnight at 4C.
    PCNA Antibody (PC10) - BSA Free NB500-106
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell whole cell lysate
    Species: Human
    Verified Customer | Posted 08/23/2015
  • Name: simon hardy
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Chicken intestine
    Species: Other
    Verified Customer | Posted 03/28/2014
  • Name: Anonymous
    Application: Immunofluorescence
    Verified Customer | Posted 02/24/2014
    PCNA - IB4 & DAPI
    PCNA Antibody (PC10) - BSA Free NB500-106
  • Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Mouse cornea
    Species: Mouse
    Verified Customer | Posted 02/06/2012
    PCNA Antibody (PC10) - BSA Free NB500-106

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PCNA Antibody (PC10) - BSA Free

Showing  1 - 3 of 3 FAQs Showing All

  • Q: Can you please tell me the specific region of immunogen of this product?

    A: This antibody was raised against whole rat PCNA protein fused to Protein A. As we have not yet epitope mapped this product, we do not know the exact immunogen recognized by this antibody.

  • Q: Do you have data/information on the use of this antibody for Drosophila (mentioned on your website as possible target species)? I would like to know if any specific treatment is needed with regard to this type of sample and the use of the antibody in WB, IP in ICC.

    A: This particular product is a widely licensed monoclonal clone. We obtained validation of its ability to detect the Drosophila protein from the following publication: PMID 22253867. You should be able to follow our general protocols for WB, IP, and ICC to detect this protein in their samples. Please see the following link for protocols.

  • Q: I am looking into preparing an exercise for an undergraduate lab that would immunostain drosophila testes. I think that PCNA Antibody (PC10) (cat # NB500-106) would be used as the primary antibody. What would you suggest to be used for the secondary? We have a fluorescent microscope and have used FITC in the past for some of our labs. Just trying to give the students a chance to visualize stained testes.

    A: After looking at the applications of this antibody, I just found that we have not established this antibody for its Immunofluorescence application in our lab yet, however, as it has been tested successfully in IHC-P by our lab and by other researchers; there is a very strong chance that it should work in IHC-P/IF application also. If you would be interested in testing this novel application, please take a look at our Innovator's Reward Program. Regarding your specific query on secondary antibody, our FITC conjugated Mouse IgG antibody with catalog # NB720-F would be a perfect choice for primary PCNA Antibody (PC10) with catalog # NB500-106.

  • Q: Can you please tell me the specific region of immunogen of this product?

    A: This antibody was raised against whole rat PCNA protein fused to Protein A. As we have not yet epitope mapped this product, we do not know the exact immunogen recognized by this antibody.

  • Q: Do you have data/information on the use of this antibody for Drosophila (mentioned on your website as possible target species)? I would like to know if any specific treatment is needed with regard to this type of sample and the use of the antibody in WB, IP in ICC.

    A: This particular product is a widely licensed monoclonal clone. We obtained validation of its ability to detect the Drosophila protein from the following publication: PMID 22253867. You should be able to follow our general protocols for WB, IP, and ICC to detect this protein in their samples. Please see the following link for protocols.

  • Q: I am looking into preparing an exercise for an undergraduate lab that would immunostain drosophila testes. I think that PCNA Antibody (PC10) (cat # NB500-106) would be used as the primary antibody. What would you suggest to be used for the secondary? We have a fluorescent microscope and have used FITC in the past for some of our labs. Just trying to give the students a chance to visualize stained testes.

    A: After looking at the applications of this antibody, I just found that we have not established this antibody for its Immunofluorescence application in our lab yet, however, as it has been tested successfully in IHC-P by our lab and by other researchers; there is a very strong chance that it should work in IHC-P/IF application also. If you would be interested in testing this novel application, please take a look at our Innovator's Reward Program. Regarding your specific query on secondary antibody, our FITC conjugated Mouse IgG antibody with catalog # NB720-F would be a perfect choice for primary PCNA Antibody (PC10) with catalog # NB500-106.

  • Q: Can you please tell me the specific region of immunogen of this product?

    A: This antibody was raised against whole rat PCNA protein fused to Protein A. As we have not yet epitope mapped this product, we do not know the exact immunogen recognized by this antibody.

  • Q: Do you have data/information on the use of this antibody for Drosophila (mentioned on your website as possible target species)? I would like to know if any specific treatment is needed with regard to this type of sample and the use of the antibody in WB, IP in ICC.

    A: This particular product is a widely licensed monoclonal clone. We obtained validation of its ability to detect the Drosophila protein from the following publication: PMID 22253867. You should be able to follow our general protocols for WB, IP, and ICC to detect this protein in their samples. Please see the following link for protocols.

  • Q: I am looking into preparing an exercise for an undergraduate lab that would immunostain drosophila testes. I think that PCNA Antibody (PC10) (cat # NB500-106) would be used as the primary antibody. What would you suggest to be used for the secondary? We have a fluorescent microscope and have used FITC in the past for some of our labs. Just trying to give the students a chance to visualize stained testes.

    A: After looking at the applications of this antibody, I just found that we have not established this antibody for its Immunofluorescence application in our lab yet, however, as it has been tested successfully in IHC-P by our lab and by other researchers; there is a very strong chance that it should work in IHC-P/IF application also. If you would be interested in testing this novel application, please take a look at our Innovator's Reward Program. Regarding your specific query on secondary antibody, our FITC conjugated Mouse IgG antibody with catalog # NB720-F would be a perfect choice for primary PCNA Antibody (PC10) with catalog # NB500-106.

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