PD-1 Antibody - BSA Free

Immunohistochemistry-Paraffin: PD-1 Antibody - BSA Free [NBP1-77276]

Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-77276] - Human brain tissue with PD-1 antibody at 2.5 ug/mL.

Western Blot: PD-1 Antibody - BSA Free [NBP1-77276] -

In vitro inhibition by the CH-4 analog of the PD-1/PD-L1 interaction. (A) HEK293 cells (left-hand panel) and Jurkat cells (right-hand panel) were treated with CH-4.7 or CH-4.9 to test cytotoxicity. Cells were seeded into 96-well plates at a density of 7 × 103/well and incubated overnight, then treated with increasing concentrations of CH-4.7 or CH-4.9 (0, 10, 20, 40, 80, or 100 uM) for 48 h. At 48 h, cell cytotoxicity WST-1 assays were performed. The x-axis indicates treatment concentrations, while the y-axis indicates the percentage of cell viability (each absolute absorbance value [abs. 450 nm–630 nm] was normalized with the controls). (B) The capacities of CH-4.7 and CH-4.9 to inhibit the PD-1/PD-L1 interaction were tested by the flow cytometry assay. The graphs present the mean +/- SD (standard deviation) values from at least three independent experiments. (C) CH-4.7 cytotoxicity in KG-1 cells. (D) Flow cytometry determined binding of the Ni–NTA-l-labeled sPD-L1 (PD-L1-Atto) complex to KG-1 cells expressing PD-1. Cell staining (FITC-subset) was blocked in the presence of CH-4.7. The experimental groups are indicated as (a) KG-1/PBS, (b) KG-1/Atto dye, (c) KG-1/sPD-L1/Atto dye, (d) KG-1/sPD-L1/Atto dye/CH-4 (10 uM), (e) KG-1/sPD-L1/Atto dye/CH-4.7 (20 uM). (E) The cell staining data from (D) are normalized and quantified as relative MFI values. (F) At 48 h, PD-1 protein (20 ug/well) expression was determined by Western blotting. The bar graphs present the mean +/- SD (standard deviation) values from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34996924), licensed under a CC-BY license. Not internally tested by Novus Biologicals.