PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76770

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies, Tagged Protein Expression

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PD-L2/B7-DC Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 16 amino acids near the center of human PD-L2/B7-DC/PDCD1LG2. The immunogen is located within amino acids 140-190 of PD-L2/B7-DC/PDCD1LG2.

Specificity

Human PD-L2/B7-DC/PDCD1LG2 has 3 isoforms, including isoform 1 (273aa, 31kD), isoform 2 (183aa, 21kD) and isoform 3 (182aa, 21kD). Mouse PD-L2/B7-DC/PDCD1LG2 has one isoform (247aa, 28kD) and Rat PD-L2/B7-DC/PDCD1LG2 also has one isoform (268aa, 30kD). NBP1-76770 can detect human isoform and mouse isoform.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free

Western Blot: PD-L2/B7-DC/PDCD1LG2 AntibodyBSA Free [NBP1-76770]

Western Blot: PD-L2/B7-DC/PDCD1LG2 AntibodyBSA Free [NBP1-76770]

Western Blot: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - Loading: 15 ug of HeLa WT cell lysates or PD-L2 KO celllysates. Antibodies: PD-L2, 4063 (4 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000dilution
Western Blot: PD-L2/B7-DC/PDCD1LG2 AntibodyBSA Free [NBP1-76770]

Western Blot: PD-L2/B7-DC/PDCD1LG2 AntibodyBSA Free [NBP1-76770]

Western Blot: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - Loading: 15 ug of lysates per lane. Antibodies: PD-L2 (4 ug/mL), competitor antibody (4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunocytochemistry/ Immunofluorescence: PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free [NBP1-76770]

Immunocytochemistry/ Immunofluorescence: PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free [NBP1-76770]

Immunocytochemistry/Immunofluorescence: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - analysis of 4% paraformaldehydefixed mouse brain cells labeling PD-L2 with this antibody at 20ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (red).
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free [NBP1-76770]

Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free [NBP1-76770]

Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - Staining of mouse brain tissue with antibody at 2.5 ug/ml.
Western Blot: PD-L2/B7-DC/PDCD1LG2 AntibodyBSA Free [NBP1-76770]

Western Blot: PD-L2/B7-DC/PDCD1LG2 AntibodyBSA Free [NBP1-76770]

Western Blot: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - Analysis of PD-L2 in Raji cell lysate with PD-L2 antibody at 0.5 and 1 ug/mL.
Western Blot: PD-L2/B7-DC/PDCD1LG2 AntibodyBSA Free [NBP1-76770]

Western Blot: PD-L2/B7-DC/PDCD1LG2 AntibodyBSA Free [NBP1-76770]

Western Blot: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - Loading: 15 ug of HeLa WT cell lysates or PD-L2 KO cell lysates. Antibodies: PD-L2, NBP1-76770 (4 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry-Paraffin: PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free [NBP1-76770]

Immunohistochemistry-Paraffin: PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free [NBP1-76770]

Immunohistochemistry-Paraffin: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - Mouse brain tissue.
PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free [NBP1-76770] -

Immunocytochemistry/ Immunofluorescence: PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free [NBP1-76770] - Validation of PD-L2/B7-DC/PDCD1LG2 in Mouse Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling PD-L2/B7-DC/PDCD1LG2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Applications for PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/mL

Western Blot

0.5-4 ug/mL

Reviewed Applications

Read 1 review rated 5 using NBP1-76770 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PD-L2/B7-DC

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PDL-2 can act to stimulate an immunogenic response through and alternative receptor from PD-1. At least three isoforms of PDL-2 are known to exist; this antibody is specific to the largest isoform. PDL-2 antibody has no cross-reactivity to PDL-2.

Long Name

Programmed Death-1 Ligand-2

Alternate Names

B7-DC, Butyrophilin-like Protein, CD273, PDCD1LG2, PDL2

Gene Symbol

PDCD1LG2

UniProt

NP_079515

Additional PD-L2/B7-DC Products

Product Documents for PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free

Customer Reviews for PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free (1)

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  • PD-L2/B7-DC/PDCD1LG2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Lung tissue
    Species: Human
    Verified Customer | Posted 06/21/2017

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Protocols

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