PDK-1 Antibody (2E2) - Azide and BSA Free

Name: PDK-1 Antibody (2E2) - Azide and BSA Free
Brand: Novus Biologicals
SKU: H00005170-M05
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # H00005170-M05

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E2

Format

Azide and BSA Free

View all PDK-1 Primary Antibodies »

Product Specifications

Immunogen

PDPK1 (NP_002604, 457 a.a. ~ 556 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ILKMGPVDKRKGLFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKNFKTFFVHTPNRTYYLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAVQ

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PDK-1 Antibody (2E2) - Azide and BSA Free

Western Blot: PDK-1 Antibody (2E2) [H00005170-M05]

Western Blot: PDK-1 Antibody (2E2) [H00005170-M05] - Analysis of PDPK1 expression in rat brain.

Western Blot: PDK-1 Antibody (2E2) [H00005170-M05]

Western Blot: PDK-1 Antibody (2E2) [H00005170-M05] - Analysis of PDPK1 expression in IMR-32.

Western Blot: PDK-1 Antibody (2E2) [H00005170-M05]

Western Blot: PDK-1 Antibody (2E2) [H00005170-M05] - Analysis of PDPK1 expression in transfected 293T cell line by PDPK1 monoclonal antibody (M05), clone 2E2. Lane 1: PDPK1 transfected lysate (Predicted MW: 48.2 KDa). Lane 2: Non-transfected lysate.

Applications for PDK-1 Antibody (2E2) - Azide and BSA Free

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

It has been used for ELISA and WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PDK-1

PDK-1 (3-phosphoinositide-dependent protein kinase, gene name PDPK1) is a 58 -68 kDa, 556 amino acid (aa) monomeric protein of the AGC serine/threonine kinase family. It is activated by phosphorylation in the presence of PtdIns(3,4,5) P3 or PtdIns(3,4) P2. Akt, S6 kinases, PKA and PKC-zeta are reported PDK-1 substrates. Through Akt, PDK-1 mediates many of the intracellular actions of insulin. Within the region used as an immunogen, human PDK-1 shares 98% aa identity with mouse and rat PDK-1. One reported isoform has an alternate start site at aa 50, while another lacking aa 238-263 is predicted to be catalytically inactive.

Long Name

Phosphoinositide Dependent Kinase-1

Alternate Names

PDK1, PDPK1, PKB kinase

Gene Symbol

PDPK1

Additional PDK-1 Products

Product Documents for PDK-1 Antibody (2E2) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for PDK-1 Antibody (2E2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.