PDK-1 Antibody - BSA Free

Name: PDK-1 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP2-48631
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-48631

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: HSLSASDTGLPQRSGSNIEQYIHDLDSNSFELDLQFSEDEKRLLLEKQAGGNPWHQFVENNLILKMGPVDKRKG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDK-1 Antibody - BSA Free

Immunohistochemistry-Paraffin: PDK-1 Antibody [NBP2-48631]

Immunohistochemistry-Paraffin: PDPK1 Antibody [NBP2-48631] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Applications for PDK-1 Antibody - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDK-1

PDK-1 (3-phosphoinositide-dependent protein kinase, gene name PDPK1) is a 58 -68 kDa, 556 amino acid (aa) monomeric protein of the AGC serine/threonine kinase family. It is activated by phosphorylation in the presence of PtdIns(3,4,5) P3 or PtdIns(3,4) P2. Akt, S6 kinases, PKA and PKC-zeta are reported PDK-1 substrates. Through Akt, PDK-1 mediates many of the intracellular actions of insulin. Within the region used as an immunogen, human PDK-1 shares 98% aa identity with mouse and rat PDK-1. One reported isoform has an alternate start site at aa 50, while another lacking aa 238-263 is predicted to be catalytically inactive.

Long Name

Phosphoinositide Dependent Kinase-1

Alternate Names

PDK1, PDPK1, PKB kinase

Gene Symbol

PDPK1

Additional PDK-1 Products

Product Documents for PDK-1 Antibody - BSA Free

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Certificate of Analysis

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Product Specific Notices for PDK-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.