Phospho-Akt (S473) Pan Specific DuoSet IC ELISA

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Citations (16)
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Phospho-Akt (S473) Pan Specific DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Length
4 hours 40 minutes (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Assay Range
156.0 - 10,000 pg/mL
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated human, mouse, and rat Akt (S473) Pan Specific in cell lysates. An immobilized capture antibody specific for Akt binds both phosphorylated and unphosphorylated Akt. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data

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Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Akt

Akt1, Akt2, and Akt3 make up a family of serine/threonine kinases, often activated downstream of growth factor receptors and PI 3-Kinase. Each Akt family member contains an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory domain. They have putative roles in cell proliferation and survival, and Akt perturbations are thought to play an important role in tumorigenesis.

Long Name:
v-Akt Murine Thymoma Viral Oncogene Homolog
Alternate Names:
RAC-ALPHA; AKT serine/threonine kinase 1; Akt; PKB; PKB-ALPHA; PRKBA; RAC

Citations for Phospho-Akt (S473) Pan Specific DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. A small-molecule PI3K? activator for cardioprotection and neuroregeneration
    Authors: Gong, GQ;Bilanges, B;Allsop, B;Masson, GR;Roberton, V;Askwith, T;Oxenford, S;Madsen, RR;Conduit, SE;Bellini, D;Fitzek, M;Collier, M;Najam, O;He, Z;Wahab, B;McLaughlin, SH;Chan, AWE;Feierberg, I;Madin, A;Morelli, D;Bhamra, A;Vinciauskaite, V;Anderson, KE;Surinova, S;Pinotsis, N;Lopez-Guadamillas, E;Wilcox, M;Hooper, A;Patel, C;Whitehead, MA;Bunney, TD;Stephens, LR;Hawkins, PT;Katan, M;Yellon, DM;Davidson, SM;Smith, DM;Phillips, JB;Angell, R;Williams, RL;Vanhaesebroeck, B;
    Species: Human
    Sample Types: Cell Lysates
  2. Identification and development of a subtype-selective allosteric AKT inhibitor suitable for clinical development
    Authors: N Page, M Wappett, CR O'Dowd, M O'Rourke, G Gavory, L Zhang, JSS Rountree, L Jordan, O Barker, H Gibson, C Boyd, S Feutren-Bu, E McLean, G Trevitt, T Harrison
    Scientific Reports, 2022-09-20;12(1):15715.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Decreased concentrations of intracellular signaling proteins in colon cancer patients with BRAF mutations
    Authors: DA Olsen, CEB Thomsen, RF Andersen, JS Madsen, A Jakobsen, I Brandslund
    Sci Rep, 2020-11-18;10(1):20113.
    Species: Human
    Sample Types: Tissue Homogenates
  4. Branched-chain ketoacid overload inhibits insulin action in the muscle
    Authors: D Biswas, KT Dao, A Mercer, AM Cowie, L Duffley, Y El Hiani, PC Kienesberg, T Pulinilkun
    J. Biol. Chem., 2020-09-02;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Enhancing the regenerative effectiveness of growth factors by local inhibition of interleukin-1 receptor signaling
    Authors: Z Julier, R Karami, B Nayer, YZ Lu, AJ Park, K Maruyama, GA Kuhn, R Müller, S Akira, MM Martino
    Sci Adv, 2020-06-12;6(24):eaba7602.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Enhancing the regenerative effectiveness of growth factors by local inhibition of interleukin-1 receptor signaling
    Authors: Z Julier, R Karami, B Nayer, YZ Lu, AJ Park, K Maruyama, GA Kuhn, R Müller, S Akira, MM Martino
    Sci Adv, 2020;6(24):eaba7602.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Authors: A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí
    Cell Mol Gastroenterol Hepatol, 2019-11-02;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  8. Rosiglitazone accelerates wound healing by improving endothelial precursor cell function and angiogenesis in db/db mice
    Authors: G Zhou, X Han, Z Wu, Q Shi, X Bao
    PeerJ, 2019-10-17;7(0):e7815.
    Species: Mouse
    Sample Types: Cell Lysates
  9. Evidence that Graves' Ophthalmopathy Immunoglobulins Do Not Directly Activate IGF-1 Receptors
    Authors: B Marcus-Sam, CC Krieger, A Boutin, G Kahaly, S Neumann, MC Gershengor
    Thyroid, 2018-04-30;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  10. Neurotensin stimulates sortilin and mTOR in human microglia inhibitable by methoxyluteolin, a potential therapeutic target for autism
    Proc Natl Acad Sci USA, 2016-09-23;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  11. The Escherichia coli subtilase cytotoxin A subunit specifically cleaves cell-surface GRP78 protein and abolishes COOH-terminal-dependent signaling.
    Authors: Ray, Rupa, de Ridder, Gustaaf, Eu, Jerry P, Paton, Adrienne, Paton, James C, Pizzo, Salvator
    J Biol Chem, 2012-07-31;287(39):32755-69.
    Species: Human
    Sample Types: Cell Lysates
  12. Protein secretion in human mammary epithelial cells following HER1 receptor activation: influence of HER2 and HER3 expression.
    Authors: Zhang Y, Gonzalez RM, Zangar RC
    BMC Cancer, 2011-02-14;11(0):69.
    Species: Human
    Sample Types: Cell Lysates
  13. Distinctive ERK and p38 signaling in remote and infarcted myocardium during post-MI remodeling in the mouse.
    Authors: Yeh CC, Li H, Malhotra D, Turcato S, Nicholas S, Tu R, Zhu BQ, Cha J, Swigart PM, Myagmar BE, Baker AJ, Simpson PC, Mann MJ
    J. Cell. Biochem., 2010-04-15;109(6):1185-91.
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. Mechanism of the salutary effects of estrogen on kupffer cell phagocytic capacity following trauma-hemorrhage: pivotal role of Akt activation.
    Authors: Hsieh CH, Nickel EA, Chen J, Schwacha MG, Choudhry MA, Bland KI, Chaudry IH
    J. Immunol., 2009-04-01;182(7):4406-14.
    Species: Mouse
    Sample Types: Cell Lysates
  15. Citrullination of CXCL12 differentially reduces CXCR4 and CXCR7 binding with loss of inflammatory and anti-HIV-1 activity via CXCR4.
    Authors: Struyf S, Noppen S, Loos T, Mortier A, Gouwy M, Verbeke H, Huskens D, Luangsay S, Parmentier M, Geboes K, Schols D, Van Damme J, Proost P
    J. Immunol., 2009-01-01;182(1):666-74.
    Species: Human
    Sample Types: Cell Lysates
  16. Citrullination of CXCL10 and CXCL11 by peptidylarginine deiminase: a naturally occurring posttranslational modification of chemokines and new dimension of immunoregulation.
    Authors: Loos T, Mortier A, Gouwy M, Ronsse I, Put W, Lenaerts JP, Van Damme J, Proost P
    Blood, 2008-07-21;112(7):2648-56.
    Species: Hamster
    Sample Types: Cell Lysates


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Phospho-Akt (S473) Pan Specific DuoSet IC ELISA
By Jenna Nguyen on 05/07/2019
Sample Tested: A7r5 rat thoracic aortic smooth muscle cell line