Phospho-Akt (S473) Pan Specific DuoSet IC ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Complete Your Research
Complete Your Experiment
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15- (96-well) plate pack sizes
- Economical alternative to Western blot
Kit Content
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
- Streptavidin-HRP
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: Akt
Akt1, Akt2, and Akt3 make up a family of serine/threonine kinases, often activated downstream of growth factor receptors and PI 3-Kinase. Each Akt family member contains an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory domain. They have putative roles in cell proliferation and survival, and Akt perturbations are thought to play an important role in tumorigenesis.
Citations for Phospho-Akt (S473) Pan Specific DuoSet IC ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Identification and development of a subtype-selective allosteric AKT inhibitor suitable for clinical development
Authors: N Page, M Wappett, CR O'Dowd, M O'Rourke, G Gavory, L Zhang, JSS Rountree, L Jordan, O Barker, H Gibson, C Boyd, S Feutren-Bu, E McLean, G Trevitt, T Harrison
Scientific Reports, 2022;12(1):15715.
Species: Human
Sample Types: Cell Culture Supernates
-
Branched-chain ketoacid overload inhibits insulin action in the muscle
Authors: D Biswas, KT Dao, A Mercer, AM Cowie, L Duffley, Y El Hiani, PC Kienesberg, T Pulinilkun
J. Biol. Chem., 2020;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Decreased concentrations of intracellular signaling proteins in colon cancer patients with BRAF mutations
Authors: DA Olsen, CEB Thomsen, RF Andersen, JS Madsen, A Jakobsen, I Brandslund
Sci Rep, 2020;10(1):20113.
Species: Human
Sample Types: Tissue Homogenates
-
Rosiglitazone accelerates wound healing by improving endothelial precursor cell function and angiogenesis in db/db mice
Authors: G Zhou, X Han, Z Wu, Q Shi, X Bao
PeerJ, 2019;7(0):e7815.
Species: Mouse
Sample Types: Cell Lysates
-
A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET
Authors: A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí
Cell Mol Gastroenterol Hepatol, 2019;0(0):.
Species: Human
Sample Types: Cell Lysates
-
Evidence that Graves' Ophthalmopathy Immunoglobulins Do Not Directly Activate IGF-1 Receptors
Authors: B Marcus-Sam, CC Krieger, A Boutin, G Kahaly, S Neumann, MC Gershengor
Thyroid, 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
-
Neurotensin stimulates sortilin and mTOR in human microglia inhibitable by methoxyluteolin, a potential therapeutic target for autism
Proc Natl Acad Sci USA, 2016;0(0):.
Species: Human
Sample Types: Cell Lysates
-
The Escherichia coli subtilase cytotoxin A subunit specifically cleaves cell-surface GRP78 protein and abolishes COOH-terminal-dependent signaling.
Authors: Ray, Rupa, de Ridder, Gustaaf, Eu, Jerry P, Paton, Adrienne, Paton, James C, Pizzo, Salvator
J Biol Chem, 2012;287(39):32755-69.
Species: Human
Sample Types: Cell Lysates
-
Protein secretion in human mammary epithelial cells following HER1 receptor activation: influence of HER2 and HER3 expression.
Authors: Zhang Y, Gonzalez RM, Zangar RC
BMC Cancer, 2011;11(0):69.
Species: Human
Sample Types: Cell Lysates
-
Distinctive ERK and p38 signaling in remote and infarcted myocardium during post-MI remodeling in the mouse.
Authors: Yeh CC, Li H, Malhotra D, Turcato S, Nicholas S, Tu R, Zhu BQ, Cha J, Swigart PM, Myagmar BE, Baker AJ, Simpson PC, Mann MJ
J. Cell. Biochem., 2010;109(6):1185-91.
Species: Mouse
Sample Types: Tissue Homogenates
-
Citrullination of CXCL12 differentially reduces CXCR4 and CXCR7 binding with loss of inflammatory and anti-HIV-1 activity via CXCR4.
Authors: Struyf S, Noppen S, Loos T, Mortier A, Gouwy M, Verbeke H, Huskens D, Luangsay S, Parmentier M, Geboes K, Schols D, Van Damme J, Proost P
J. Immunol., 2009;182(1):666-74.
Species: Human
Sample Types: Cell Lysates
-
Mechanism of the salutary effects of estrogen on kupffer cell phagocytic capacity following trauma-hemorrhage: pivotal role of Akt activation.
Authors: Hsieh CH, Nickel EA, Chen J, Schwacha MG, Choudhry MA, Bland KI, Chaudry IH
J. Immunol., 2009;182(7):4406-14.
Species: Mouse
Sample Types: Cell Lysates
-
Citrullination of CXCL10 and CXCL11 by peptidylarginine deiminase: a naturally occurring posttranslational modification of chemokines and new dimension of immunoregulation.
Authors: Loos T, Mortier A, Gouwy M, Ronsse I, Put W, Lenaerts JP, Van Damme J, Proost P
Blood, 2008;112(7):2648-56.
Species: Hamster
Sample Types: Cell Lysates
FAQs
No product specific FAQs exist for this product, however you may
View all ELISA FAQsDuoSet Ancillary Reagent Kits
Supplemental ELISA Products
Reviews for Phospho-Akt (S473) Pan Specific DuoSet IC ELISA
There are currently no reviews for this product. Be the first to review Phospho-Akt (S473) Pan Specific DuoSet IC ELISA and earn rewards!
Have you used Phospho-Akt (S473) Pan Specific DuoSet IC ELISA?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image