Phospho-STAT6 (Y641) PE-conjugated Antibody

Catalog #: IC3717P Datasheet / COA / SDS
Newer Version Available: IC37173R
NEW

Discontinued Product

IC3717P has been discontinued and is replaced by IC37173R. IC37173P.

Detection of Phospho-STAT6 (Y641) in Daudi Human Cell Line by Flow Cytometry.
1 Image
Product Details
FAQs
Reviews

Phospho-STAT6 (Y641) PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human STAT6 when phosphorylated at Y641.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human STAT6 Y641 site.
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Phospho-STAT6 (Y641) in Daudi Human Cell Line by Flow Cytometry. View Larger

Detection of Phospho-STAT6 (Y641) in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line, resting (light orange filled histogram), or treated with Recombinant Human IL-4 (Catalog # 204-IL, dark orange filled histogram) was stained with Rabbit Anti-Human Phospho-STAT6 (Y641) PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3717P) or isotype control antibody (Catalog # IC105P, open histogram). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: STAT6

Signal Transducer and Activator of Transcription 6 (STAT6) mediates the signaling of cytokines such as IL-4 and IL-13. STAT6 acts as a signal transducer in the cytoplasm, and upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN4GAA. Knockout studies in mice suggest that STAT6 functions in the differentiation of T helper 2 (Th2) cells, the expression of cell surface markers, and class switching of immunoglobulins.

Long Name
Signal Transducer and Activator of Transcription 6
Entrez Gene IDs
6778 (Human); 20852 (Mouse); 362896 (Rat)
Alternate Names
D12S1644; EC 2.4.1.227; EC 2.7.7.6; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin-4 induced; STAT, interleukin4-induced; STAT6; STAT6B; STAT6C; transcription factor IL-4 STAT

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Phospho-STAT6 (Y641) PE-conjugated Antibody

There are currently no reviews for this product. Be the first to review Phospho-STAT6 (Y641) PE-conjugated Antibody and earn rewards!

Have you used Phospho-STAT6 (Y641) PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review