Phospho-STAT6 (Y641) PE-conjugated Antibody

IC3717P has been discontinued and is replaced by IC37173PIC37173R.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human STAT6 when phosphorylated at Y641.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide containing human STAT6 Y641 site.
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Phospho-STAT6 (Y641) in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line, resting (light orange filled histogram), or treated with Recombinant Human IL‑4 (Catalog # 204-IL, dark orange filled histogram) was stained with Rabbit Anti-Human Phospho-STAT6 (Y641) PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3717P) or isotype control antibody (Catalog # IC105P, open histogram). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: STAT6

Signal Transducer and Activator of Transcription 6 (STAT6) mediates the signaling of cytokines such as IL-4 and IL-13. STAT6 acts as a signal transducer in the cytoplasm, and upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN4GAA. Knockout studies in mice suggest that STAT6 functions in the differentiation of T helper 2 (Th2) cells, the expression of cell surface markers, and class switching of immunoglobulins.

  • Long Name:
    Signal Transducer and Activator of Transcription 6
  • Entrez Gene IDs:
    6778 (Human); 20852 (Mouse); 362896 (Rat)
  • Alternate Names:
    D12S1644; EC 2.4.1.227; EC 2.7.7.6; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin4-induced; STAT6; STAT6B; STAT6C; transcription factor IL-4 STAT
Related Research Areas

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Order Details

    IC3717P has been replaced by
    IC37173P
    IC37173R

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