PI 3-Kinase p110 beta/PIK3CB Antibody (5A9) - BSA Free
Novus Biologicals | Catalog # NBP3-26383
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5A9 expressed in HEK293
Format
BSA Free
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Product Specifications
Immunogen
A synthesized peptide derived from Human PI 3-Kinase p110 beta/PIK3CB [UniProt P42338]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PI 3-Kinase p110 beta/PIK3CB Antibody (5A9) - BSA Free
Immunoprecipitation: PI 3-Kinase p110 beta/PIK3CB Antibody (5A9) [NBP3-26383] -
Immunoprecipitation: PI 3-Kinase p110 beta/PIK3CB Antibody (5A9) [NBP3-26383] - Immunoprecipitating PI 3-Kinase p110 beta/PIK3CB in K562 whole cell lysate.Lane 1: Rabbit control IgG instead of NBP3-26383 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26383 (2ug)+ K562 whole cell lysate (500ug).
Lane 3: K562 whole cell lysate (10ug).
Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody (5A9) [NBP3-26383] -
Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody (5A9) [NBP3-26383] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate.All lanes: PI 3-Kinase p110 beta/PIK3CB Antibody at 1: 1500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 123 kDa
Observed band size: 110 kDa
Applications for PI 3-Kinase p110 beta/PIK3CB Antibody (5A9) - BSA Free
Application
Recommended Usage
Immunoprecipitation
1:200-1:1000
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: PI 3-Kinase p110 beta
Long Name
Protein Tyrosine Phosphatase 1B
Alternate Names
PI 3Kinase p110 beta, PIK3CB
Gene Symbol
PIK3CB
Additional PI 3-Kinase p110 beta Products
Product Documents for PI 3-Kinase p110 beta/PIK3CB Antibody (5A9) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PI 3-Kinase p110 beta/PIK3CB Antibody (5A9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Immunoprecipitation Protocol
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Common Cytokine Receptor Gamma-Chain Family Signaling Pathways
IL-2 Signaling Pathways
IL-4 Signaling Pathways
IL-7 Signaling Pathways
IL-9 Signaling Pathways
IL-15 Signaling Pathways
IL-21 Signaling Pathways
mTOR Signaling Pathway
Notch Signaling Pathways
TGF-beta Signaling Pathways
VEGF - VEGF R2 Signaling Pathways