PI 3-Kinase p85 alpha Antibody (3A10) - Azide and BSA Free

Name: PI 3-Kinase p85 alpha Antibody (3A10) - Azide and BSA Free
Brand: Novus Biologicals
SKU: H00005295-M01
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # H00005295-M01

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 3A10

Format

Azide and BSA Free

View all PI 3-Kinase p85 alpha Primary Antibodies »

Product Specifications

Immunogen

PI 3-Kinase p85 alpha (AAH30815, 1 a.a. ~ 454 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MYNTVWNMEDLDLEYAKTDINCGTDLMFYIEMDPPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHKYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PI 3-Kinase p85 alpha Antibody (3A10) - Azide and BSA Free

Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01]

Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of PIK3R1 expression in transfected 293T cell line by PIK3R1 monoclonal antibody (M01), clone 3A10.Lane 1: PIK3R1 transfected lysate (Predicted MW: 50 KDa).Lane 2: Non-transfected lysate.

Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01]

Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - PIK3R1 monoclonal antibody (M01), clone 3A10 Analysis of PIK3R1 expression in HeLa.

Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01]

Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - PIK3R1 monoclonal antibody (M01), clone 3A10. Analysis of PIK3R1 expression in PC-12.

Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01]

Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - PIK3R1 monoclonal antibody (M01), clone 3A10. Analysis of PIK3R1 expression in Raw 264.7.

Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01]

Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - PIK3R1 monoclonal antibody (M01), clone 3A10. Analysis of PIK3R1 expression in NIH/3T3.

Proximity Ligation Assay: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01]

Proximity Ligation Assay: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of protein-protein interactions between EGFR and PIK3R1. HeLa cells were stained with anti-EGFR rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01]

Proximity Ligation Assay: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of protein-protein interactions between FLT1 and PIK3R1. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01]

Proximity Ligation Assay: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of protein-protein interactions between CRKL and PIK3R1. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Immunoprecipitation: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01]

Immunoprecipitation: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of PIK3R1 transfected lysate using anti-PIK3R1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PIK3R1 monoclonal antibody.

Sandwich ELISA: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Detection limit for recombinant GST tagged PIK3R1 is approximately 0.1ng/ml as a capture antibody.

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Applications for PI 3-Kinase p85 alpha Antibody (3A10) - Azide and BSA Free

Application

Recommended Usage

Western Blot

1:500

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PI 3-Kinase p85 alpha

Phosphatidylinositol 3-kinase (PI3K) p85 alpha is a regulatory subunit that heterodimerizes with a catalytic subunit to form a Class IA PI3K enzyme complex, which plays an important role in the immune system (1,2). The P13K pathway is involved in many diverse processes including growth, metabolism, proliferation, and survival (2). PI3Ks are typically activated by cytokine receptors and are responsible for phosphorylation of the 3'-hydroxyl group of PI and its derivatives (3). p85 alpha is one of five regulatory subunit proteins which also includes p55 alpha, p50 alpha, p85 beta, and p55 gamma, and can bind to two of the three catalytic subunits (p110 alpha or p110 delta) (1,2). p85 alpha, p55 alpha, and p50 alpha proteins are all synthesized by the same PIK3R1 gene by alternative splicing (1). Structurally, PI 3-Kinase p85 alpha contains SRC homology 3 (SH3 domain), followed by a Bcr homology (BH) domain flanked by two proline-rich regions, then a N-terminal SH2 domain, an inter-SH2 domain, and C-terminal SH2 domain (1,2). PI 3-Kinase p85 alpha protein consists of 724 amino acids (aa) in length with a theoretical molecular weight of 83.5 kDa (2,4). The primary role for the p85 subunit is interaction with cell surface receptors and acts as an adapter for the stabilization and recruitment of the p110 catalytic subunit to the plasma membrane (1,3). Additionally, p85 has been shown to function in both interleukin-2 receptor (IL2R) and erythropoietin receptor (EpoR) endocytosis (3).

The PI3K pathway functions in a broad range of cellular processes, so it is understandable that pathway dysfunction can lead to an array of diseases and disorders (2,5). Elevated PI3K signaling is a key feature of many cancers (5). PI3K pathway dysregulation has also been implicated in neurological, metabolic, and cardiovascular disorders (5). Furthermore, both overactivation or under-activation of the PI3K delta (p85 alpha subunit + p110 delta subunit) pathway has been shown to cause immunodeficiency and pathologies related to immune system dysfunction (2). Therapeutics to target the PI3K pathway and treat related cancers include PI3K inhibitors and, specifically, isoform-selective inhibitors which have a lot of promise when used as part of a combination therapy (5).

References

1. Okkenhaug, K., & Vanhaesebroeck, B. (2001). New responsibilities for the PI3K regulatory subunit p85 alpha. Science's STKE : signal transduction knowledge environment. https://doi.org/10.1126/stke.2001.65.pe1

2. Nunes-Santos, C. J., Uzel, G., & Rosenzweig, S. D. (2019). PI3K pathway defects leading to immunodeficiency and immune dysregulation. The Journal of allergy and clinical immunology. https://doi.org/10.1016/j.jaci.2019.03.017

3. Chen, P. H., Yao, H., & Huang, L. J. (2017). Cytokine Receptor Endocytosis: New Kinase Activity-Dependent and -Independent Roles of PI3K. Frontiers in endocrinology. https://doi.org/10.3389/fendo.2017.00078

4. Uniprot (P27986)

5. Fruman, D. A., Chiu, H., Hopkins, B. D., Bagrodia, S., Cantley, L. C., & Abraham, R. T. (2017). The PI3K Pathway in Human Disease. Cell. https://doi.org/10.1016/j.cell.2017.07.029

Long Name

PI 3-Kinase p85 alpha

Alternate Names

GRB1, PI 3Kinase p85 alpha, PIK3R1

Entrez Gene IDs

5295 (Human)

Gene Symbol

PIK3R1

OMIM

171833 (Human)

UniProt

AAH30815 (Human)

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Product Specific Notices for PI 3-Kinase p85 alpha Antibody (3A10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.