PLGF Antibody (3B10) - Azide and BSA Free
Novus Biologicals | Catalog # NB110-96418
![Western Blot: PLGF Antibody (3B10)Azide and BSA Free [NB110-96418]](https://resources.rndsystems.com/images/products/PLGF-Antibody-3B10-Western-Blot-NB110-96418-img0021.jpg "Western Blot: PLGF Antibody (3B10)Azide and BSA Free [NB110-96418]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3B10
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant human PlGF-2
Specificity
P1GF-2 (3B10)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for PLGF Antibody (3B10) - Azide and BSA Free
Western Blot: PLGF Antibody (3B10)Azide and BSA Free [NB110-96418]
Western Blot: PLGF Antibody (3B10) [NB110-96418] - Figure 1: Western analysis with recombinant human PlGF-1 and PlGF-2 derived from insect cells. The monoclonal mouse anti-human PlGF antibody #3B10 recognizes solely the PlGF-2 isoform.![Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]](https://resources.rndsystems.com/images/products/PLGF-Antibody-3B10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-96418-img0022.jpg "Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]")
Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]
Immunocytochemistry/Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418] - Immunofluorescence staining of mouse VEGF-A and PLGF in primary mouse endothelial cells (mEC) using a monoclonal antibodies directed against human VEGF-A and human PLGF [NB110-96418], respectively. As secondary antibody goat anti-mouse ALEXA Flour 488 (1:600) was used.![Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]](https://resources.rndsystems.com/images/products/PLGF-Antibody-3B10-Immunocytochemistry-Immunofluorescence-NB110-96418-img0014.jpg "Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]")
Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]
Immunocytochemistry/Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418] - PLGF Antibody (3B10) [NB110-96418] - Immunofluorescence staining of PlGF in a mixture of primary human dermal lymphatic endothelial cells (HDLEC) and human dermal normal fibroblasts with monoclonal mouse anti-human PlGF #3B10. The cells were pretreated with 0,1% Triton X100. Control with goat anti-mouse ALEXA Flour 488.![Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]](https://resources.rndsystems.com/images/products/PLGF-Antibody-3B10-Immunocytochemistry-Immunofluorescence-NB110-96418-img0018.jpg "Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]")
Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]
Immunocytochemistry/Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418] - PLGF Antibody (3B10) [NB110-96418] - Immunofluorescence staining of PlGF in primary human dermal fibroblast cells (HDNF) with monoclonal mouse anti-human PlGF #3B10 (upper panel). Control with goat anti-mouse ALEXA Flour 488 (lower panel).![Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]](https://resources.rndsystems.com/images/products/PLGF-Antibody-3B10-Immunocytochemistry-Immunofluorescence-NB110-96418-img0019.jpg "Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]")
Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]
Immunocytochemistry/Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418] - PLGF Antibody (3B10) [NB110-96418] - Immunofluorescence staining of human PLGF in HUVE cells with the monoclonal mouse anti-human PlGF #3B10 antibody (upper panel). Control with goat anti-mouse ALEXA Flour 488 (lower panel).Applications for PLGF Antibody (3B10) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1-2 ug/ml
Western Blot
1-2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Calculators
Background: PlGF
Long Name
Placenta Growth Factor
Alternate Names
PGF, PGFL
Gene Symbol
PGF
Additional PlGF Products
Product Documents for PLGF Antibody (3B10) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PLGF Antibody (3B10) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for PLGF Antibody (3B10) - Azide and BSA Free
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Q:
Does the Human PlGF Quantikine® ELISA Kit, Catalog # DPG00, detect free or total PlGF?
A:
Soluble receptors or other binding proteins present in biological samples do not necessarily interfere with the measurement of ligands in samples. However, until the factors have been tested in the Quantikine® Immunoassay, the possibility of interference cannot be excluded. We cannot claim Catalog # DPG00 to be a "free" or "total" PlGF detecting kit, since all possible binding factors have not been tested.
Specificity testing performed using rhFlt-1/Fc (VEGF R1) for interference demonstrated interference at levels greater than 2000 pg/mL. Details of this testing can be found in the DPG00 product insert. Most samples will have lower levels of sFlt-1 than 2000 pg/ml.
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