PLGF Antibody (JA63-15)

Novus Biologicals | Catalog # NBP2-67067

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JA63-15 expressed in HEK293
View all PlGF Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human PLGF aa 48-97 / 221. (SwissProt: P49763 Human; SwissProt: P49764 Mouse; SwissProt: Q63434 Rat)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLGF Antibody (JA63-15)

Western Blot: PLGF Antibody (JA63-15) [NBP2-67067]

Western Blot: PLGF Antibody (JA63-15) [NBP2-67067]

Western Blot: PLGF Antibody (JA63-15) [NBP2-67067] - Western blot analysis of PLGF on JAR cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: PLGF Antibody (JA63-15) [NBP2-67067]

Immunocytochemistry/ Immunofluorescence: PLGF Antibody (JA63-15) [NBP2-67067]

Immunocytochemistry/Immunofluorescence: PLGF Antibody (JA63-15) [NBP2-67067] - Staining PLGF in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067]

Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067]

Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of paraffin-embedded rat kidney tissue using anti-PLGF antibody. Counter stained with hematoxylin.
Western Blot: PLGF Antibody (JA63-15) [NBP2-67067]

Western Blot: PLGF Antibody (JA63-15) [NBP2-67067]

Western Blot: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of PLGF on SH-SY5Y cell lysate using anti-PLGF antibody at 1/1,000 dilution.
Western Blot: PLGF Antibody (JA63-15) [NBP2-67067]

Western Blot: PLGF Antibody (JA63-15) [NBP2-67067]

Western Blot: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of PLGF on JAR cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: PLGF Antibody (JA63-15) [NBP2-67067]

Immunocytochemistry/ Immunofluorescence: PLGF Antibody (JA63-15) [NBP2-67067]

Immunocytochemistry/Immunofluorescence: PLGF Antibody (JA63-15) [NBP2-67067] - Staining PLGF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067]

Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067]

Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of paraffin-embedded human colon cancer tissue using anti-PLGF antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067]

Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067]

Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of paraffin-embedded human liver cancer tissue using anti-PLGF antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067]

Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067]

Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of paraffin-embedded mouse orchis tissue using anti-PLGF antibody. Counter stained with hematoxylin.

Applications for PLGF Antibody (JA63-15)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PlGF

PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It belongs to the cysteine-knot superfamily. PLGF is expressed in placental tissues, colon and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor.

Long Name

Placenta Growth Factor

Alternate Names

PGF, PGFL

Gene Symbol

PGF

Additional PlGF Products

Product Documents for PLGF Antibody (JA63-15)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PLGF Antibody (JA63-15)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PLGF Antibody (JA63-15)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PLGF Antibody (JA63-15)

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  • Q:

    Does the Human PlGF Quantikine® ELISA Kit, Catalog # DPG00, detect free or total PlGF?

    A:

    Soluble receptors or other binding proteins present in biological samples do not necessarily interfere with the measurement of ligands in samples. However, until the factors have been tested in the Quantikine® Immunoassay, the possibility of interference cannot be excluded. We cannot claim Catalog # DPG00 to be a "free" or "total" PlGF detecting kit, since all possible binding factors have not been tested. 

    Specificity testing performed using rhFlt-1/Fc (VEGF R1) for interference demonstrated interference at levels greater than 2000 pg/mL. Details of this testing can be found in the DPG00 product insert. Most samples will have lower levels of sFlt-1 than 2000 pg/ml.

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