Porcine IL-12/IL-23 p40 Antibody

Catalog #: MAB912 Datasheet / COA / SDS

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MAB912
MAB912-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Porcine IL-12/IL-23 p40 Antibody Summary

Species Reactivity

Porcine

Specificity

Detects porcine IL-12/IL-23 p40 in direct ELISAs and Western blots. In Western blots (under reducing conditions), porcine IL-12/IL-23 p40 will detect the p40 subunit, but not the p35 subunit. In Western blots, less than 10% cross-reactivity with recombinant mouse IL‑12 and recombinant human IL-12 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 116237

Purification

Protein A or G purified from ascites

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant porcine IL‑12 p35/p40 heterodimer

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Complete Your Research

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Complete Your Experiment

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Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Porcine IL‑12/IL‑23 p40

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12/IL-23 p40

IL-12 p70 is a heterodimeric cytokine composed of one 35 kDa and one 40 kDa subunit. The 40 kDa subunit also occurs in IL-23. IL-12 is produced by macrophages and B lymphocytes and has multiple effects on T cells and natural killer (NK) cells.

Long Name

Interleukin 12/Interleukin 23 p40

Entrez Gene IDs

3593 (Human); 16160 (Mouse); 64546 (Rat); 397076 (Porcine); 403976 (Canine); 102124858 (Cynomolgus Monkey); 493741 (Feline); 694747 (Rhesus Macaque)

Alternate Names

CLMF p40; CLMF; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 p40; IL-12 subunit p40; IL12B; IL-12B; IL-12BNK cell stimulatory factor chain 2; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NKSF; NKSF2; NKSF2IL12, subunit p40