Prokineticin 2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43853

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Prokineticin 2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide derived from Cter region of human PK2

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Prokineticin 2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Prokineticin 2 Antibody [NBP2-43853]

Immunocytochemistry/ Immunofluorescence: Prokineticin 2 Antibody [NBP2-43853]

Immunocytochemistry/Immunofluorescence: Prokineticin 2 Antibody [NBP2-43853] - Localization of Bv8/PK2 in fasciculata cells.

Applications for Prokineticin 2 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:50-1:1000

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:200-1:1000
Application Notes
Use in IHC reported in scientific literature (PMID: 30046389).

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute in distilled water to final concentration of 1mg/ml.

Formulation

0.1M Tris, 0.1M Glycine and 2% Sucrose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Lyophilized antibody can be kept at 4C for up to 3 months and should be kept at -20C for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20C) or for long-term storage (-80C). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Prokineticin 2

Prokineticin 2 is known to regulate many different biological functions, including neurogenesis, smooth muscle contractility, angiogenesis and circadian rhythm. In serving the latter role, prokineticin 2 functions as an output molecule from the suprachiasmatic nucleus (SCN) of the hypothalamus, that transmits behavioral rhythms, but may also function locally within the SCN to synchronize output. Prokineticin 2 expression is induced by CLOCK and BMAL1 heterodimers and light, and is inhibited by period genes (PER1, PER2 and PER3) and cryptochrome genes (CRY1 and CRY2). Expression is reported in the SCN and among a few other discrete brain areas, including the islands of Calleja, media l preoptic area of the hypothalamus and the shell of the nucleus accumbens as well as in the testis, prostate and, at lower levels, in the small intestine.

Long Name

Prokineticin-2

Alternate Names

BV8, PROK2, Protein Bv8 homolog

Gene Symbol

PROK2

Additional Prokineticin 2 Products

Product Documents for Prokineticin 2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Prokineticin 2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Prokineticin 2 Antibody - Azide and BSA Free

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Protocols

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