RAB14 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84720

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RAB14 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GQERFRAVTRSYYRGAAGALMVYDITRRSTYNHLSSWLTDARNLTNPNTVIILIGNKADLEAQRDVTYEEAKQFAEENGLLFLEASAKTGENVEDAFLEAAKKIYQNIQDGSLDLNAAESGVQHKPSAPQGGRLTSEPQPQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAB14 Antibody - BSA Free

RAB14 Antibody - BSA Free Western Blot Shows RAB14 Specificity Using Knockdown Cell Line.

Western Blot Shows RAB14 Specificity Using Knockdown Cell Line.

Western blot shows lysates of DMS 53 parental cell line and RAB14 knockdown DMS 53 cell line (KD). Nitrocellulose membrane was probed with RAB14 Antibody (Catalog # NBP1-84720) followed by HRP-conjugated secondary antibody. A specific band was detected for RAB14 at approximately 23.8 kDa (as indicated) in the parental DMS 53 cell line but is significantly reduced in knockdown DMS 53 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
Immunocytochemistry/ Immunofluorescence: RAB14 Antibody [NBP1-84720]

Immunocytochemistry/ Immunofluorescence: RAB14 Antibody [NBP1-84720]

Immunocytochemistry/Immunofluorescence: RAB14 Antibody [NBP1-84720] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]

Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]

Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]

Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]

Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]

Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]

Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720] - Staining of human gastrointestinal shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]

Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720]

Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
RAB14 Antibody - BSA Free Western Blot: RAB14 Antibody - BSA Free [NBP1-84720]

Western Blot: RAB14 Antibody - BSA Free [NBP1-84720]

Analysis in human cell line RH-30.

Applications for RAB14 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAB14

RAB14 belongs to the large RAB family of low molecular mass GTPases that are involved in intracellular membrane trafficking. These proteins act as molecular switches that flip between an inactive GDP-bound state and an active GTP-bound state in which they recruit downstream effector proteins onto membranes (Junutula et al., 2004 (PubMed 15004230)).(supplied by OMIM)

Alternate Names

bA165P4.3 (member RAS oncogene family), F protein-binding protein 1, FBP, RAB-14, RAB14, member RAS oncogene family, ras-related protein Rab-14, small GTP binding protein RAB14

Gene Symbol

RAB14

Additional RAB14 Products

Product Documents for RAB14 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RAB14 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RAB14 Antibody - BSA Free

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Protocols

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