Rat CNTF Biotinylated Antibody

  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat CNTF in ELISAs and Western blots. In sandwich immunoassays, less than 3% cross‑reactivity with recombinant human CNTF is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant rat CNTF
    Ala2-Met200
    Accession # P20294.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Rat CNTF (Catalog # 557-NT)
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Rat CNTF Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Rat CNTF Antibody (Catalog # MAB557)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Rat CNTF Biotinylated Antibody (Catalog # BAF557)
  • ELISA Standard
     
    Recombinant Rat CNTF Protein (Catalog # 557-NT)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
CNTF in Rat Brain. CNTF was detected in perfusion fixed frozen sections of rat brain (midbrain) using Goat Anti-Rat CNTF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF557) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell bodies and neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CNTF
Ciliary neurotrophic factor (CNTF) is structurally related to IL-6, IL-11, LIF, CLC, and OSM. CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types, including dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons.
  • Long Name:
    Ciliary Neurotrophic Factor
  • Entrez Gene IDs:
    1270 (Human); 25707 (Rat)
  • Alternate Names:
    ciliary neurotrophic factor; CNTF; HCNTF
Related Research Areas
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Isotype Controls
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Normal Goat IgG Biotinylated Control

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NorthernLights Streptavidin NL557

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NorthernLights Streptavidin NL637

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NorthernLights Streptavidin NL493

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MagCellect Streptavidin Ferrofluid

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EvenCoat Streptavidin Coated Plates, 5 Pack

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