Rat CNTF Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF557
Rat CNTF Biotinylated Antibody in Data
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Rat CNTF Biotinylated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat CNTF in ELISAs and Western blots. In sandwich immunoassays, less than 3% cross‑reactivity with recombinant human CNTF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat CNTF
Ala2-Met200
Accession # P20294.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat CNTF (Catalog # 557-NT)
Immunohistochemistry
5-15 µg/mL
See below

Rat CNTF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Rat CNTF Antibody (Catalog # MAB557)

Standard: Recombinant Rat CNTF Protein (Catalog # 557-NT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry CNTF in Rat Brain. View Larger

CNTF in Rat Brain. CNTF was detected in perfusion fixed frozen sections of rat brain (midbrain) using Goat Anti-Rat CNTF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF557) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell bodies and neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNTF

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons, and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

Long Name
Ciliary Neurotrophic Factor
Entrez Gene IDs
1270 (Human); 25707 (Rat)
Alternate Names
ciliary neurotrophic factor; CNTF; HCNTF

Product Datasheets

FAQs

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Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Staining Reagents

Supplemental Cell Selection Products

Supplemental ELISA Products

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