Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunodepletion

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 34738
View all CNTF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat CNTF
Ala2-Met200
Accession # P20294.1

Specificity

Detects rat CNTF in ELISAs and Western blots. In sandwich immunoassays, less than 3% cross-reactivity with recombinant human CNTF is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<1.0 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Rat CNTF Antibody (34738)

Cell Proliferation Induced by CNTF and Neutralization by Rat CNTF Antibody.

Cell Proliferation Induced by CNTF and Neutralization by Rat CNTF Antibody.

Recombinant Rat CNTF (Catalog # 557-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat CNTF (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CNTF Monoclonal Antibody (Catalog # MAB557). The ND50 is typically 1-3 µg/mL.
Rat CNTF ELISA Standard Curve

Rat CNTF ELISA Standard Curve

Recombinant Rat CNTF (Catalog # 557-NT) was serially diluted and captured by Mouse Anti-Rat CNTF Monoclonal Antibody (Catalog # MAB557) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Rat CNTF Antibody (34738)

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize CNTF-induced proliferation in the TF‑1 human erythroleukemic cell line. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 200 ng/mL Recombinant Rat CNTF.

Rat CNTF Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Rat CNTF Biotinylated Antibody (Catalog # BAF557)
  • Standard: Recombinant Rat CNTF Protein (Catalog # 557-NT)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CNTF

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons, and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

Long Name

Ciliary Neurotrophic Factor

Alternate Names

ciliary neurotrophic factor, HCNTF

Entrez Gene IDs

1270 (Human); 12803 (Mouse); 25707 (Rat)

Gene Symbol

CNTF

UniProt

P20294.1

Additional CNTF Products

Product Documents for Rat CNTF Antibody (34738)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat CNTF Antibody (34738)

For research use only

Citations for Rat CNTF Antibody (34738)

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