Rat ICAM-1/CD54 Quantikine ELISA Kit

(19 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL)
  • Sensitivity
    4.1 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant rat sICAM-1
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Rat sICAM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat sICAM-1 levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant rat sICAM-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring rat sICAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural rat sICAM-1.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation4.313297.61935


The recovery of rat sICAM-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=8) 99 88-104
EDTA Plasma (n=5) 103 91-117
Serum (n=6) 103 83-119
To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of rat sICAM-1 in each matrix were diluted with Calibrator Diluent and then assayed.
Rat ICAM-1/CD54 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ICAM-1/CD54
ICAM-1 (intercellular adhesion molecule-1), also known as CD54, is a transmembrane protein that is upregulated on endothelial and epithelial cells at sites of inflammation. It mediates the vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). It also binds several non-integrin ligands including CD43/Sialophorin, Fibrinogen, Hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes. Soluble ICAM-1 promotes angiogenesis and serves an indicator of vascular endothelial cell activation or damage. Elevated levels of soluble ICAM-1 are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, increased abdominal fat mass, hypertension, liver disease, certain malignancies, and cerebral malaria.
    • Long Name
      Intercellular Adhesion Molecule 1
    • Entrez Gene IDs
      3383 (Human); 15894 (Mouse); 25464 (Rat);
    • Alternate Names
      BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 19
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    Sample Type
    1. Astaxanthin Inhibits Expression of Retinal Oxidative Stress and Inflammatory Mediators in Streptozotocin-Induced Diabetic Rats.
      Authors: Yeh P, Huang H, Yang C, Yang W, Yang C
      PLoS ONE, 2016;11(1):e0146438.
      Species: Rat
      Sample Type: Aqueous humor
    2. Sex difference in urocortin production is contributory to the gender disparity in a rat model of vasculitis induced by sodium laurate.
      Authors: Wan, Rong, Jin, Lai, Zhu, Chao, Liu, Yunxin, Li, Li, Guo, Rui, Li, Shengnan
      Endocrinology, 2013;154(12):4663-74.
      Species: Rat
      Sample Type: Plasma
    3. High dietary intake of retinol leads to bone marrow hypoxia and diaphyseal endosteal mineralization in rats.
      Authors: Lind T, Lind PM, Jacobson A, Hu L, Sundqvist A, Risteli J, Yebra-Rodriguez A, Rodriguez-Navarro A, Andersson G, Melhus H
      Bone, 2011;48(3):496-506.
      Species: Rat
      Sample Type: Serum
    4. Hyperglycemia enhances excessive superoxide anion radical generation, oxidative stress, early inflammation, and endothelial injury in forebrain ischemia/reperfusion rats.
      Authors: Tsuruta R, Fujita M, Ono T, Koda Y, Koga Y, Yamamoto T, Nanba M, Shitara M, Kasaoka S, Maruyama I, Yuasa M, Maekawa T
      Brain Res., 2010;1309(0):155-63.
    5. Cholinergic agonist physostigmine suppresses excessive superoxide anion radical generation in blood, oxidative stress, early inflammation, and endothelial injury in rats with forebrain ischemia/reperfusion.
      Authors: Kutsuna S, Tsuruta R, Fujita M, Todani M, Yagi T, Ogino Y, Igarashi M, Takahashi K, Izumi T, Kasaoka S, Yuasa M, Maekawa T
      Brain Res., 2010;1313(0):242-9.
      Species: Rat
      Sample Type: Tissue Homogenates
    6. Nuclear factor-kappaB activation by edema inhibits intestinal contractile activity.
      Authors: Uray KS, Wright Z, Kislitsyna K, Xue H, Cox CS
      Crit. Care Med., 2010;38(3):861-70.
      Species: Rat
      Sample Type: Cell Lysates
    7. Poly(ADP-ribose) polymerase (PARP) inhibition counteracts multiple manifestations of kidney disease in long-term streptozotocin-diabetic rat model.
      Authors: Shevalye H, Stavniichuk R, Xu W, Zhang J, Lupachyk S, Maksimchyk Y, Drel VR, Floyd EZ, Slusher B, Obrosova IG
      Biochem. Pharmacol., 2010;79(7):1007-14.
      Species: Rat
      Sample Type: Tissue Homogenates
    8. Rho kinase inhibition by fasudil ameliorates diabetes-induced microvascular damage.
      Authors: Arita R, Hata Y, Nakao S, Kita T, Miura M, Kawahara S, Zandi S, Almulki L, Tayyari F, Shimokawa H, Hafezi-Moghadam A, Ishibashi T
      Diabetes, 2009;58(1):215-26.
      Species: Rat
      Sample Type: Cell Lysates
    9. Elevation of jugular venous superoxide anion radical is associated with early inflammation, oxidative stress, and endothelial injury in forebrain ischemia-reperfusion rats.
      Authors: Aki HS, Fujita M, Yamashita S, Fujimoto K, Kumagai K, Tsuruta R, Kasaoka S, Aoki T, Nanba M, Murata H, Yuasa M, Maruyama I, Maekawa T
      Brain Res., 2009;1292(0):180-90.
      Species: Rat
      Sample Type: Tissue Homogenates
    10. Maternal diet influences DNA damage, aortic telomere length, oxidative stress, and antioxidant defense capacity in rats.
      Authors: Tarry-Adkins JL, Martin-Gronert MS, Chen JH, Cripps RL, Ozanne SE
      FASEB J., 2008;22(6):2037-44.
      Species: Rat
      Sample Type: Serum
    11. Vascular adhesion protein-1 blockade suppresses choroidal neovascularization.
      Authors: Noda K, She H, Nakazawa T, Hisatomi T, Nakao S, Almulki L, Zandi S, Miyahara S, Ito Y, Thomas KL, Garland RC, Miller JW, Gragoudas ES, Mashima Y, Hafezi-Moghadam A
      FASEB J., 2008;22(8):2928-35.
      Species: Rat
      Sample Type: Tissue Homogenates
    12. Role of sex steroids in modulating tumor necrosis factor alpha-induced changes in vascular function and blood pressure.
      Authors: LaMarca BD, Chandler DL, Grubbs L, Bain J, McLemore GR, Granger JP, Ryan MJ
      Am. J. Hypertens., 2007;20(11):1216-21.
      Species: Rat
      Sample Type: Serum
    13. Triamcinolone acetonide protects the rat retina from STZ-induced acute inflammation and early vascular leakage.
      Authors: Kim YH, Choi MY, Kim YS, Park CH, Lee JH, Chung IY, Yoo JM, Choi WS, Cho GJ, Kang SS
      Life Sci., 2007;81(14):1167-73.
      Species: Rat
      Sample Type: Tissue Homogenates
    14. Mechanism of the nongenomic effects of estrogen on intestinal myeloperoxidase activity following trauma-hemorrhage: up-regulation of the PI-3K/Akt pathway.
      Authors: Yu HP, Hsieh YC, Suzuki T, Choudhry MA, Schwacha MG, Bland KI, Chaudry IH
      J. Leukoc. Biol., 2007;82(3):774-80.
      Species: Rat
      Sample Type: Tissue Homogenates
    15. Study of plasma protein C and inflammatory pathways: biomarkers for dimethylnitrosamine-induced liver fibrosis in rats.
      Authors: Saha JK, Xia J, Sandusky GE, Chen YF, Gerlitz B, Grinnell B, Jakubowski JA
      Eur. J. Pharmacol., 2007;575(1):158-67.
      Species: Rat
      Sample Type: Plasma
    16. Anti-inflammatory effects of inhaled nitric oxide are optimized at lower oxygen concentration in experimental Klebsiella pneumoniae pneumonia.
      Authors: Sun Z, Sun B, Wang X, Wang W, Zhu L
      Inflamm. Res., 2006;55(10):430-40.
      Species: Rat
      Sample Type: Tissue Homogenates
    17. Suppression of ocular inflammation in endotoxin-induced uveitis by inhibiting nonproteolytic activation of prorenin.
      Authors: Satofuka S, Ichihara A, Nagai N, Yamashiro K, Koto T, Shinoda H, Noda K, Ozawa Y, Inoue M, Tsubota K, Suzuki F, Oike Y, Ishida S
      Invest. Ophthalmol. Vis. Sci., 2006;47(6):2686-92.
      Species: Rat
      Sample Type: Tissue Homogenates
    18. Effect of anemonin on NO, ET-1 and ICAM-1 production in rat intestinal microvascular endothelial cells.
      Authors: Duan H, Zhang Y, Xu J, Qiao J, Suo Z, Hu G, Mu X
      J Ethnopharmacol, 2006;104(3):362-6.
      Species: Rat
      Sample Type: Cell Culture Supernates
    19. Maintenance of lung myeloperoxidase activity in proestrus females after trauma-hemorrhage: upregulation of heme oxygenase-1.
      Authors: Yu HP, Yang S, Hsieh YC, Choudhry MA, Bland KI, Chaudry IH
      Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(3):L400-6.
      Species: Rat
      Sample Type: Tissue Homogenates
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