Rat ICAM-1/CD54 Quantikine ELISA Kit

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RIC100
Rat ICAM-1/CD54 Standard Curve
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Product Details
Procedure
Citations (20)
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Rat ICAM-1/CD54 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL)
Sensitivity
4.1 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant rat sICAM-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Rat sICAM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat sICAM-1 levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant rat sICAM-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring rat sICAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural rat sICAM-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 47 39 47
Mean 87 271 617 80 307 560
Standard Deviation 4.3 13 29 7.6 19 35
CV% 4.9 4.8 4.7 9.5 6.2 6.2

Recovery

The recovery of rat sICAM-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=8) 99 88-104
EDTA Plasma (n=5) 103 91-117
Serum (n=6) 103 83-119

Linearity

To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of rat sICAM-1 in each matrix were diluted with Calibrator Diluent and then assayed.
Rat ICAM-1/CD54 ELISA Linearity

Data Examples

Rat ICAM-1/CD54 Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ICAM-1/CD54

ICAM-1 (intercellular adhesion molecule-1), also known as CD54, is a transmembrane protein that is upregulated on endothelial and epithelial cells at sites of inflammation. It mediates the vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). It also binds several non-integrin ligands including CD43/Sialophorin, Fibrinogen, Hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes. Soluble ICAM-1 promotes angiogenesis and serves an indicator of vascular endothelial cell activation or damage. Elevated levels of soluble ICAM-1 are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, increased abdominal fat mass, hypertension, liver disease, certain malignancies, and cerebral malaria.

Long Name:
Intercellular Adhesion Molecule 1
Entrez Gene IDs:
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names:
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Rat ICAM-1/CD54 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Disrupting the blood-brain barrier by focused ultrasound induces sterile inflammation
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  2. Astaxanthin Inhibits Expression of Retinal Oxidative Stress and Inflammatory Mediators in Streptozotocin-Induced Diabetic Rats.
    Authors: Yeh P, Huang H, Yang C, Yang W, Yang C
    PLoS ONE, 2016;11(1):e0146438.
    Species: Rat
    Sample Types: Aqueous humor
  3. Sex difference in urocortin production is contributory to the gender disparity in a rat model of vasculitis induced by sodium laurate.
    Authors: Wan, Rong, Jin, Lai, Zhu, Chao, Liu, Yunxin, Li, Li, Guo, Rui, Li, Shengnan
    Endocrinology, 2013;154(12):4663-74.
    Species: Rat
    Sample Types: Plasma
  4. High dietary intake of retinol leads to bone marrow hypoxia and diaphyseal endosteal mineralization in rats.
    Authors: Lind T, Lind PM, Jacobson A, Hu L, Sundqvist A, Risteli J, Yebra-Rodriguez A, Rodriguez-Navarro A, Andersson G, Melhus H
    Bone, 2011;48(3):496-506.
    Species: Rat
    Sample Types: Serum
  5. Hyperglycemia enhances excessive superoxide anion radical generation, oxidative stress, early inflammation, and endothelial injury in forebrain ischemia/reperfusion rats.
    Authors: Tsuruta R, Fujita M, Ono T, Koda Y, Koga Y, Yamamoto T, Nanba M, Shitara M, Kasaoka S, Maruyama I, Yuasa M, Maekawa T
    Brain Res., 2010;1309(0):155-63.
  6. Poly(ADP-ribose) polymerase (PARP) inhibition counteracts multiple manifestations of kidney disease in long-term streptozotocin-diabetic rat model.
    Authors: Shevalye H, Stavniichuk R, Xu W, Zhang J, Lupachyk S, Maksimchyk Y, Drel VR, Floyd EZ, Slusher B, Obrosova IG
    Biochem. Pharmacol., 2010;79(7):1007-14.
    Species: Rat
    Sample Types: Tissue Homogenates
  7. Cholinergic agonist physostigmine suppresses excessive superoxide anion radical generation in blood, oxidative stress, early inflammation, and endothelial injury in rats with forebrain ischemia/reperfusion.
    Authors: Kutsuna S, Tsuruta R, Fujita M, Todani M, Yagi T, Ogino Y, Igarashi M, Takahashi K, Izumi T, Kasaoka S, Yuasa M, Maekawa T
    Brain Res., 2010;1313(0):242-9.
    Species: Rat
    Sample Types: Tissue Homogenates
  8. Nuclear factor-kappaB activation by edema inhibits intestinal contractile activity.
    Authors: Uray KS, Wright Z, Kislitsyna K, Xue H, Cox CS
    Crit. Care Med., 2010;38(3):861-70.
    Species: Rat
    Sample Types: Cell Lysates
  9. Rho kinase inhibition by fasudil ameliorates diabetes-induced microvascular damage.
    Authors: Arita R, Hata Y, Nakao S, Kita T, Miura M, Kawahara S, Zandi S, Almulki L, Tayyari F, Shimokawa H, Hafezi-Moghadam A, Ishibashi T
    Diabetes, 2009;58(1):215-26.
    Species: Rat
    Sample Types: Cell Lysates
  10. Elevation of jugular venous superoxide anion radical is associated with early inflammation, oxidative stress, and endothelial injury in forebrain ischemia-reperfusion rats.
    Authors: Aki HS, Fujita M, Yamashita S, Fujimoto K, Kumagai K, Tsuruta R, Kasaoka S, Aoki T, Nanba M, Murata H, Yuasa M, Maruyama I, Maekawa T
    Brain Res., 2009;1292(0):180-90.
    Species: Rat
    Sample Types: Tissue Homogenates
  11. Maternal diet influences DNA damage, aortic telomere length, oxidative stress, and antioxidant defense capacity in rats.
    Authors: Tarry-Adkins JL, Martin-Gronert MS, Chen JH, Cripps RL, Ozanne SE
    FASEB J., 2008;22(6):2037-44.
    Species: Rat
    Sample Types: Serum
  12. Vascular adhesion protein-1 blockade suppresses choroidal neovascularization.
    Authors: Noda K, She H, Nakazawa T, Hisatomi T, Nakao S, Almulki L, Zandi S, Miyahara S, Ito Y, Thomas KL, Garland RC, Miller JW, Gragoudas ES, Mashima Y, Hafezi-Moghadam A
    FASEB J., 2008;22(8):2928-35.
    Species: Rat
    Sample Types: Tissue Homogenates
  13. Study of plasma protein C and inflammatory pathways: biomarkers for dimethylnitrosamine-induced liver fibrosis in rats.
    Authors: Saha JK, Xia J, Sandusky GE, Chen YF, Gerlitz B, Grinnell B, Jakubowski JA
    Eur. J. Pharmacol., 2007;575(1):158-67.
    Species: Rat
    Sample Types: Plasma
  14. Triamcinolone acetonide protects the rat retina from STZ-induced acute inflammation and early vascular leakage.
    Authors: Kim YH, Choi MY, Kim YS, Park CH, Lee JH, Chung IY, Yoo JM, Choi WS, Cho GJ, Kang SS
    Life Sci., 2007;81(14):1167-73.
    Species: Rat
    Sample Types: Tissue Homogenates
  15. Role of sex steroids in modulating tumor necrosis factor alpha-induced changes in vascular function and blood pressure.
    Authors: LaMarca BD, Chandler DL, Grubbs L, Bain J, McLemore GR, Granger JP, Ryan MJ
    Am. J. Hypertens., 2007;20(11):1216-21.
    Species: Rat
    Sample Types: Serum
  16. Mechanism of the nongenomic effects of estrogen on intestinal myeloperoxidase activity following trauma-hemorrhage: up-regulation of the PI-3K/Akt pathway.
    Authors: Yu HP, Hsieh YC, Suzuki T, Choudhry MA, Schwacha MG, Bland KI, Chaudry IH
    J. Leukoc. Biol., 2007;82(3):774-80.
    Species: Rat
    Sample Types: Tissue Homogenates
  17. Maintenance of lung myeloperoxidase activity in proestrus females after trauma-hemorrhage: upregulation of heme oxygenase-1.
    Authors: Yu HP, Yang S, Hsieh YC, Choudhry MA, Bland KI, Chaudry IH
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(3):L400-6.
    Species: Rat
    Sample Types: Tissue Homogenates
  18. Effect of anemonin on NO, ET-1 and ICAM-1 production in rat intestinal microvascular endothelial cells.
    Authors: Duan H, Zhang Y, Xu J, Qiao J, Suo Z, Hu G, Mu X
    J Ethnopharmacol, 2006;104(3):362-6.
    Species: Rat
    Sample Types: Cell Culture Supernates
  19. Suppression of ocular inflammation in endotoxin-induced uveitis by inhibiting nonproteolytic activation of prorenin.
    Authors: Satofuka S, Ichihara A, Nagai N, Yamashiro K, Koto T, Shinoda H, Noda K, Ozawa Y, Inoue M, Tsubota K, Suzuki F, Oike Y, Ishida S
    Invest. Ophthalmol. Vis. Sci., 2006;47(6):2686-92.
    Species: Rat
    Sample Types: Tissue Homogenates
  20. Anti-inflammatory effects of inhaled nitric oxide are optimized at lower oxygen concentration in experimental Klebsiella pneumoniae pneumonia.
    Authors: Sun Z, Sun B, Wang X, Wang W, Zhu L
    Inflamm. Res., 2006;55(10):430-40.
    Species: Rat
    Sample Types: Tissue Homogenates

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