Mouse Integrin  beta 1/CD29 Antibody

2.3 out of 5

3 Customer Ratings

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• (3)

• Mouse Integrin  beta 1/CD29 Antibody

  Name: Anonymous

  Application: Immunocytochemistry/Immunofluorescence

  Sample Tested: Endothelial cells

  Species: Mouse

  Verified Customer | Posted 01/25/2022

  

• Mouse Integrin  beta 1/CD29 Antibody

  Name: Anonymous

  Application: Immunocytochemistry/Immunofluorescence

  Sample Tested: Pancreatic cancer tissue

  Species: Human

  Verified Customer | Posted 10/12/2021

  Not-so-negative control IF staining of rat anti-mouse integrin beta 1/CD29 at 15ug/mL on FFPE human tissue section, incubated at 4C overnight, and followed by Invitrogen Goat anti-Rat Alexa 488 (green) for 1 hour. Counterstained for mitochondria (red) and DAPI (blue).

  
  Bio-Techne Response

  Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction. This customer needed an anti-mouse specific antibody that could be used in an immunohistochemistry (IHC) application as a negative control to differentiate transplanted human cells from surrounding mouse tissue. The MAB2405 datasheet makes no statement regarding potential cross-reactivity between human and mouse Integrin beta 1 when analyzing natural samples by IHC. Cross-reactivity has only been examined using a recombinant human Integrin beta 1 protein in Western Blotting. Because the anti-mouse Integrin beta 1 antibody displayed some putative cross-reactivity with human Integrin beta 1 protein in IHC samples, other suitable antibodies were discussed and offered as substitutes. None met the customer's dual needs of specific localized expression and zero guaranteed cross-reactivity with the human version of the protein in natural samples studied by the IHC application.

• Mouse Integrin  beta 1/CD29 Antibody

  Name: Anonymous

  Application: Immunocytochemistry/Immunofluorescence

  Sample Tested: Primary enteric nervous system (ENS) cells

  Species: Mouse

  Verified Customer | Posted 03/06/2019

  Integrin B1 antibody was used at the highest concentration (25 µg/ml) with O/N incubation at 4 degrees Celsius, using a secondary antibody chicken anti-rat Alexa 594 (Thermofisher). Dapi was used to stain the cell nuclei. A TUBB3 antibody with green labeling was used to visualize enteric neurons. Signal from Integrin B1 could not be detected where it was expected.

  
  Bio-Techne Response

  Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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