Mouse Integrin  beta 1/CD29 Antibody

Catalog # Availability Size / Price Qty
MAB2405
MAB2405-SP
Detection of Integrin  beta 1/CD29 in B16‑F1 Mouse Cell Line by Flow Cytometry.
2 Images
Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (3)

Mouse Integrin  beta 1/CD29 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin beta 1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin  beta 1, recombinant mouse (rm) Integrin  beta 2, rmIntegrin  beta 3, rmIntegrin  beta 4, or rmIntegrin  beta 6 is observed.
Source
Monoclonal Rat IgG2A Clone # 265917
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1/CD29
Gln21-Asp728
Accession # P09055
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Integrin  beta 1/CD29 under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
Perfusion fixed frozen sections of mouse spinal cord
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Integrin  beta 1/CD29 antibody in B16-F1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin beta 1/CD29 in B16‑F1 Mouse Cell Line by Flow Cytometry. B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Immunocytochemistry Integrin  beta 1/CD29 antibody in L-929 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Integrin beta 1/CD29 in L‑929 Mouse Cell Line. Integrin beta 1/CD29 was detected in immersion fixed L-929 mouse fibroblast cell line using Rat Anti-Mouse Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

The Integrin beta 1 subunit, also known as CD29, associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes.

Entrez Gene IDs
3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)
Alternate Names
CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA

Product Datasheets

Citations for Mouse Integrin  beta 1/CD29 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
Filter your results:

Filter by:

  1. The RGD-binding integrins alphavbeta6 and alphavbeta8 are receptors for mouse adenovirus-1 and -3 infection
    Authors: M Bieri, R Hendrickx, M Bauer, B Yu, T Jetzer, B Dreier, PRE Mittl, J Sobek, A Plückthun, UF Greber, S Hemmi
    PloS Pathogens, 2021;17(12):e1010083.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. The Higher Sensitivity of GABAergic Compared to Glutamatergic Neurons to Growth-Promoting C3bot Treatment Is Mediated by Vimentin
    Authors: A Adolf, P Turko, A Rohrbeck, I Just, I Vida, G Ahnert-Hil, M Höltje
    Front Cell Neurosci, 2020;14(0):596072.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  3. Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo
    Authors: M Reed, AC Luissint, V Azcutia, S Fan, MN O'Leary, M Quiros, J Brazil, A Nusrat, CA Parkos
    Nat Commun, 2019;10(1):5004.
    Species: Mouse
    Sample Types: Cells
    Applications: IF
  4. Bush-like integrin filament networks associated with hyaloid vasculature in murine neonate eyes
    Authors: T Iwanaga, J Nio-Kobaya, H Takahashi-
    Biomed. Res., 2019;40(2):79-85.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Autologous transplantation of adipose-derived stem cells improves functional recovery of skeletal muscle without direct participation in new myofiber formation
    Authors: A Gorecka, S Salemi, D Haralampie, F Moalli, D Stroka, D Candinas, D Eberli, L Brügger
    Stem Cell Res Ther, 2018;9(1):195.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice: Novel implication for vascular atrophy
    Authors: A Kishimoto, S Kimura, J Nio-Kobaya, H Takahashi-, AM Park, T Iwanaga
    Exp. Eye Res., 2018;172(0):1-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Antibody-based targeting of alternatively spliced tissue factor: a new approach to impede the primary growth and spread of pancreatic ductal adenocarcinoma
    Oncotarget, 2016;7(18):25264-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Absence of keratin 8 confers a paradoxical microflora-dependent resistance to apoptosis in the colon.
    Authors: Habtezion A, Toivola DM, Asghar MN, Kronmal GS, Brooks JD, Butcher EC, Omary MB
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(4):1445-50.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Crawling Phagocytes Recruited in the Brain Vasculature after Pertussis Toxin Exposure through IL6, ICAM1 and ITGalphaM.
    Authors: Richard JF, Roy M, Audoy-Remus J, Tremblay P, Vallieres L
    Brain Pathol., 2011;21(0):661.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse Integrin  beta 1/CD29 Antibody

Average Rating: 2.3 (Based on 3 Reviews)

5 Star
33.33%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
66.67%

Have you used Mouse Integrin  beta 1/CD29 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse Integrin  beta 1/CD29 Antibody
By Anonymous on 01/25/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Endothelial cells Species: Mouse

Mouse Integrin  beta 1/CD29 Antibody
By Anonymous on 10/12/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Pancreatic cancer tissue Species: Human

Not-so-negative control IF staining of rat anti-mouse integrin beta 1/CD29 at 15ug/mL on FFPE human tissue section, incubated at 4C overnight, and followed by Invitrogen Goat anti-Rat Alexa 488 (green) for 1 hour. Counterstained for mitochondria (red) and DAPI (blue).
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
UPDATE: This customer needed an anti-mouse specific antibody that could be used in an immunohistochemistry (IHC) application as a negative control to differentiate transplanted human cells from surrounding mouse tissue. The MAB2405 datasheet makes no statement regarding potential cross-reactivity between human and mouse Integrin beta 1 when analyzing natural samples by IHC. Cross-reactivity has only been examined using a recombinant human Integrin beta 1 protein in Western Blotting. Because the anti-mouse Integrin beta 1 antibody displayed some putative cross-reactivity with human Integrin beta 1 protein in IHC samples, other suitable antibodies were discussed and offered as substitutes. None met the customer’s dual needs of specific localized expression and zero guaranteed cross-reactivity with the human version of the protein in natural samples studied by the IHC application.


Mouse Integrin  beta 1/CD29 Antibody
By Anonymous on 03/06/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Primary enteric nervous system (ENS) cells Species: Mouse

Integrin B1 antibody was used at the highest concentration (25 µg/ml) with O/N incubation at 4 degrees Celsius, using a secondary antibody chicken anti-rat Alexa 594 (Thermofisher). Dapi was used to stain the cell nuclei. A TUBB3 antibody with green labeling was used to visualize enteric neurons. Signal from Integrin B1 could not be detected where it was expected.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***