Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit

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Rat IL-1 beta/IL-1F2 ELISA Standard Curve
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Citations (108)
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Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (17 uL), EDTA Plasma (17 uL)
Sensitivity
5 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant rat IL-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Rat IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-1 beta in cell culture supernates, serum, and EDTA plasma. It contains recombinant rat IL-1 beta and antibodies raised against recombinant rat IL-1 beta. This immunoassay has been shown to quantitate the recombinant rat factor accurately.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 83.4 253 610 82.3 249 626
Standard Deviation 7.3 9.9 24 4.7 10.3 27
CV% 8.8 3.9 3.9 5.7 4.1 4.4

Recovery

The recovery of rat IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 100 89-110
EDTA Plasma (n=5) 98 89-111
Serum (n=5) 108 97-120

Linearity

To assess the linearity of the assay, five or more samples spiked with various concentrations of rat IL-1 beta in each matrix were diluted with Calibrator Diluent and then assayed.
Rat IL-1 beta/IL-1F2 ELISA Linearity

Data Examples

Rat IL-1 beta/IL-1F2 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

The Interleukin 1 (IL-1) family of proteins consists of the classic members IL-1 alpha, IL-1 beta, and IL-1ra, plus IL-18, IL-33, and IL-1F5-10. IL-1 alpha and IL-1 beta bind to the same cell surface receptors and share biological functions (1). IL-1 is not produced by unstimulated cells of healthy mice with the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system. In response to inflammatory agents, infections, or microbial endotoxins, however, a dramatic increase in the production of IL-1 by macrophages and various other cell types is observed. IL-1 beta plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. Inappropriate or prolonged production of IL-1 has been implicated in a variety of pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases (2-5).

 IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both are synthesized as 31 kDa precursors that are subsequently cleaved into mature proteins of approximately 17.5 kDa (6, 7). Cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response (2, 8). Neither IL-1 alpha nor IL-1 beta contains a typical hydrophobic signal peptide (9-11), but evidence suggests that these factors can be secreted by non-classical pathways (12, 13). A portion of unprocessed IL-1 alpha can be presented on the cell membrane and may retain biological activity (14). The precursor form of IL-1 beta, unlike the IL-1 alpha precursor, shows little or no biological activity in comparison to the processed form (13, 15). Both unprocessed and mature forms of IL-1 beta are exported from the cell. 
 IL-1 alpha and IL-1 beta exert their effects through immunoglobulin superfamily receptors that additionally bind IL-1ra. The 80 kDa transmembrane type I receptor (IL-1 RI) is expressed on T cells, fibroblasts, keratinocytes, endothelial cells, synovial lining cells, chondrocytes, and hepatocytes (16, 17). The 68 kDa transmembrane type II receptor (IL-1 RII) is expressed on B cells, neutrophils, and bone marrow cells (18). The two IL-1 receptor types show approximately 28% homology in their extracellular domains but differ significantly in that the type II receptor has a cytoplasmic domain of only 29 amino acids (aa), whereas the type I receptor has a 217 aa cytoplasmic domain. IL-1 RII does not appear to signal in response to IL-1 and may function as a decoy receptor that attenuates IL-1 function (19). The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction (20). IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 (21, 22). Soluble forms of both IL-1 RI and IL-1 RII have been detected in human plasma, synovial fluids, and the conditioned media of several human cell lines (23, 24). In addition, IL-1 binding proteins that resemble soluble IL-1 RII are encoded by vaccinia and cowpox viruses (25).

Long Name:
Interleukin 1 beta
Entrez Gene IDs:
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names:
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

108 Citations: Showing 1 - 10
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  1. Long non-coding RNA GAS5 aggravates myocardial depression in mice with sepsis via the microRNA-449b/HMGB1 axis and the NF-&kappaB signaling pathway
    Authors: H Gao, H Ma, M Gao, A Chen, S Zha, J Yan
    Bioscience Reports, 2021;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  2. Down-regulation of SNHG16 alleviates the acute lung injury in sepsis rats through miR-128-3p/HMGB3 axis
    Authors: J Sun, K Xin, C Leng, J Ge
    BMC pulmonary medicine, 2021;21(1):191.
    Species: Rat
    Sample Types: Tissue Homogenates
  3. Long-Term Dipeptidyl Peptidase 4 Inhibition Worsens Hypertension and Renal and Cardiac Abnormalities in Obese Spontaneously Hypertensive Heart Failure Rats
    Authors: EK Jackson, Z Mi, DG Gillespie, D Cheng, SP Tofovic
    Journal of the American Heart Association, 2021;10(6):e020088.
    Species: Rat
    Sample Types: Plasma
  4. Isoflurane reduces septic neuron injury by HO?1?mediated abatement of inflammation and apoptosis
    Authors: L Zhang, X Zhang, T Wu, X Pan, Z Wang
    Molecular Medicine Reports, 2021;23(2):.
    Species: Mouse
    Sample Types: Serum
  5. Dexmedetomidine alleviates blunt chest trauma and hemorrhagic shock?resuscitation?induced acute lung injury through inhibiting the NLRP3 inflammasome
    Authors: T Ming, M Yuan, Q Kong, Q Huang, Z Xia, X Wu
    Mol Med Rep, 2020;22(3):2507-2515.
    Species: Rat
    Sample Types: Serum
  6. Moderate-intensity physical activity reduces systemic inflammation and maintains cardiorespiratory function following chronic particulate matter2.5 exposure in rats
    Authors: A van Wavere, MJ Duncan, F Coulson, A Fenning
    Toxicol Rep, 2020;7(0):93-100.
    Species: Rat
    Sample Types: Serum
  7. Comparison of the acute toxicity, analgesic and anti-inflammatory activities and chemical composition changes in Rhizoma anemones Raddeanae caused by vinegar processing
    Authors: SS Wang, SY Zhou, XY Xie, L Zhao, Y Fu, GZ Cai, JY Gong
    BMC Complement Med Ther, 2020;20(1):7.
    Species: Rat
    Sample Types: Serum
  8. Anti?inflammatory activity of 3,5,6,7,3',4'?hexamethoxyflavone via repression of the NF?&kappaB and MAPK signaling pathways in LPS?stimulated RAW264.7 cells
    Authors: ES Son, JW Park, SH Kim, HR Park, W Han, OC Kwon, JY Nam, SH Jeong, CS Lee
    Mol Med Rep, 2020;22(3):1985-1993.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. Long non-coding RNA ANRIL knockdown suppresses apoptosis and pro-inflammatory cytokines while enhancing neurite outgrowth via binding microRNA-125a in a cellular model of Alzheimer's disease
    Authors: B Zhou, L Li, X Qiu, J Wu, L Xu, W Shao
    Mol Med Rep, 2020;22(2):1489-1497.
    Species: Rat
    Sample Types: Cell Culture Supernates
  10. Histamine 2/3 receptor agonists alleviate perioperative neurocognitive disorders by inhibiting microglia activation through the PI3K/AKT/FoxO1 pathway in aged rats
    Authors: YN Chen, HH Sha, YW Wang, Q Zhou, P Bhuiyan, NN Li, YN Qian, HQ Dong
    J Neuroinflammation, 2020;17(1):217.
    Species: Rat
    Sample Types: Cell Culture Supernates
  11. Continuous administration of a p38&alpha inhibitor during the subacute phase after transient ischemia-induced stroke in the rat promotes dose-dependent functional recovery accompanied by increase in brain BDNF protein level
    Authors: JJ Alam, M Krakovsky, U Germann, A Levy
    PLoS ONE, 2020;15(12):e0233073.
    Species: Rat
    Sample Types: Tissue Culture Supernates
  12. Methane Ameliorates Lipopolysaccharide-Induced Acute Orchitis by Anti-inflammatory, Antioxidative, and Antiapoptotic Effects via Regulation of the PK2/PKR1 Pathway
    Authors: C Huang, W Zhang, A Sun, X Zhang, J Guo, R Ji, L Qiao, X Sun, D Zhao
    Oxid Med Cell Longev, 2020;2020(0):7075836.
    Species: Rat
    Sample Types: Cell Culture Supernates
  13. N?acetyl cysteine inhibits the lipopolysaccharide?induced inflammatory response in bone marrow mesenchymal stem cells by suppressing the TXNIP/NLRP3/IL?1&beta signaling pathway
    Authors: X Wang, M Jiang, X He, B Zhang, W Peng, L Guo
    Molecular Medicine Reports, 2020;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  14. Inhibition of HtrA2 alleviates inflammatory response and cell apoptosis in lipopolysaccharide?induced acute pneumonia in rats
    Authors: X Wang
    Molecular Medicine Reports, 2020;0(0):.
  15. Inhibitor of RAGE and glucose?induced inflammation in bone marrow mesenchymal stem cells: Effect and mechanism of action
    Authors: M Jiang, X Wang, P Wang, W Peng, B Zhang, L Guo
    Molecular Medicine Reports, 2020;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  16. Anticolitic Effect of Berberine in Rat Experimental Model: Impact of PGE2/p38 MAPK Pathways
    Authors: L Jia, K Xue, J Liu, OA Habotta, L Hu, AE Abdel Mone
    Mediators Inflamm, 2020;2020(0):9419085.
    Species: Rat
    Sample Types: Cell Culture Supernates
  17. Sex Differences in Vagus Nerve Stimulation Effects on Rat Cardiovascular and Immune Systems
    Authors: F Yaghouby, K Jang, U Hoang, S Asgari, S Vasudevan
    Front Neurosci, 2020;14(0):560668.
    Species: Rat
    Sample Types: Plasma
  18. Substrate Elasticity Exerts Functional Effects on Primary Microglia
    Authors: SJ Blaschke, S Demir, A König, JA Abraham, SU Vay, M Rabenstein, DN Olschewski, C Hoffmann, M Hoffmann, N Hersch, R Merkel, B Hoffmann, M Schroeter, GR Fink, MA Rueger
    Front Cell Neurosci, 2020;14(0):590500.
    Species: Rat
    Sample Types: Cell Culture Supernates
  19. SNHG16 aggravates chronic constriction injury-induced neuropathic pain in rats via binding with miR-124-3p and miR-141-3p to upregulate JAG1
    Authors: H Li, L Fan, Y Zhang, Y Cao, X Liu
    Brain Res Bull, 2020;165(0):228-237.
    Species: Rat
    Sample Types: Cell Culture Supernates
  20. Dietary Saturated Fatty Acids Modulate Pain Behaviour in Trauma-Induced Osteoarthritis in Rats
    Authors: S Sekar, SK Panchal, NK Ghattamane, L Brown, R Crawford, Y Xiao, I Prasadam
    Nutrients, 2020;12(2):.
    Species: Rat
    Sample Types: Plasma
  21. Low-Energy Extracorporeal Shock Wave Therapy Ameliorates Kidney Function in Diabetic Nephropathy
    Authors: CC Hsiao, WH Huang, KH Cheng, CT Lee
    Oxid Med Cell Longev, 2019;2019(0):8259645.
    Species: Rat
    Sample Types: Tissue Lysate
  22. Neuroinflammation induced by the peptide amyloid-? (25-35) increase the presence of galectin-3 in astrocytes and microglia and impairs spatial memory
    Authors: E Ramírez, C Sánchez-Ma, MA Mayoral, L Mendieta, V Alatriste, A Patricio-M, ID Limón
    Neuropeptides, 2019;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  23. Anti-inflammatory effect of afatinib (an EGFR-TKI) on OGD-induced neuroinflammation
    Authors: YJ Chen, CC Hsu, YJ Shiao, HT Wang, YL Lo, AMY Lin
    Sci Rep, 2019;9(1):2516.
    Species: Rat
    Sample Types: Cell Culture Supernates
  24. NLRP3 inflammasome activation in platelets in response to sepsis
    Authors: DC Cornelius, CH Baik, OK Travis, DL White, CM Young, W Austin Pie, CA Shields, B Poudel, JM Williams
    Physiol Rep, 2019;7(9):e14073.
    Species: Rat
    Sample Types: Plasma
  25. Low-Energy Extracorporeal Shock Wave Ameliorates Streptozotocin Induced Diabetes and Promotes Pancreatic Beta Cells Regeneration in a Rat Model
    Authors: CC Hsiao, CC Lin, YS Hou, JY Ko, CJ Wang
    Int J Mol Sci, 2019;20(19):.
    Species: Rat
    Sample Types: Tissue Lysates
  26. Hemorrhagic Shock Sensitized the Diaphragm to Ventilator-Induced Dysfunction through the Activation of IL-6/JAK/STAT Signaling-Mediated Autophagy in Rats
    Authors: LJ Zhang, SZ Ni, XL Zhou, Y Zhao
    Mediators Inflamm., 2019;2019(0):3738409.
    Species: Rat
    Sample Types: Tissue Homogenates
  27. Nrf2 Suppression Delays Diabetic Wound Healing Through Sustained Oxidative Stress and Inflammation
    Authors: M Li, H Yu, H Pan, X Zhou, Q Ruan, D Kong, Z Chu, H Li, J Huang, X Huang, A Chau, W Xie, Y Ding, P Yao
    Front Pharmacol, 2019;10(0):1099.
    Species: Rat
    Sample Types: Serum
  28. Neutrophil activation causes tumor regression in Walker 256 tumor-bearing rats
    Authors: WMT Kuwabara, J Andrade-Si, JNB Pereira, JH Scialfa, J Cipolla-Ne
    Sci Rep, 2019;9(1):16524.
    Species: Rat
    Sample Types: BALF
  29. Chronic agomelatine treatment prevents comorbid depression in the post-status epilepticus model of acquired epilepsy through suppression of inflammatory signaling
    Authors: J Tchekalaro, D Atanasova, L Kortenska, M Atanasova, N Lazarov
    Neurobiol. Dis., 2018;115(0):127-144.
    Species: Rat
    Sample Types: Plasma
  30. Green tea extract attenuates LPS-induced retinal inflammation in rats
    Authors: JL Ren, QX Yu, WC Liang, PY Leung, TK Ng, WK Chu, CP Pang, SO Chan
    Sci Rep, 2018;8(1):429.
    Species: Rat
    Sample Types: Vitreous Humor
  31. Levistilide A Ameliorates NLRP3 Expression Involving the Syk-p38/JNK Pathway and Peripheral Obliterans in Rats
    Authors: H Guo, L Sun, S Ling, JW Xu
    Mediators Inflamm., 2018;2018(0):7304096.
    Species: Rat
    Sample Types: Serum
  32. Protective Role of UCP2 in Oxidative Stress and Apoptosis during the Silent Phase of an Experimental Model of Epilepsy Induced by Pilocarpine
    Authors: MRH Dutra, RDS Feliciano, KR Jacinto, TLF Gouveia, E Brigidio, AJ Serra, M Morris, MDG Naffah-Maz, JA Silva
    Oxid Med Cell Longev, 2018;2018(0):6736721.
    Species: Rat
    Sample Types: Tissue Homogenates
  33. Regulation of the NLRP3 inflammasome and macrophage pyroptosis by the p38 MAPK signaling pathway in a mouse model of acute lung injury
    Authors: D Li, W Ren, Z Jiang, L Zhu
    Mol Med Rep, 2018;0(0):.
    Species: Mouse
    Sample Types: BALF
  34. MiR-10 targets NgR to modulate the proliferation of microglial cells and the secretion of inflammatory cytokines
    Authors: W Wang, R Zhan, J Zhou, J Wang, S Chen
    Exp. Mol. Pathol., 2018;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  35. Local Delivery of ?-Elemene Improves Locomotor Functional Recovery by Alleviating Endoplasmic Reticulum Stress and Reducing Neuronal Apoptosis in Rats with Spinal Cord Injury
    Authors: J Wang, H Li, Y Ren, Y Yao, J Hu, M Zheng, Y Ding, YY Chen, Y Shen, LL Wang, Y Zhu
    Cell. Physiol. Biochem., 2018;49(2):595-609.
    Species: Rat
    Sample Types: Tissue Homogenates
  36. Valproate reduces neuroinflammation and neuronal death in a rat chronic constriction injury model
    Authors: JY Chen, LW Chu, KI Cheng, SL Hsieh, YS Juan, BN Wu
    Sci Rep, 2018;8(1):16457.
    Species: Rat
    Sample Types: Tissue Homogenates
  37. Hepatokine ?1-Microglobulin Signaling Exacerbates Inflammation and Disturbs Fibrotic Repair in Mouse Myocardial Infarction
    Authors: D Hakuno, M Kimura, S Ito, J Satoh, Y Nakashima, T Horie, Y Kuwabara, M Nishiga, Y Ide, O Baba, H Nishi, T Nakao, T Nishino, F Nakazeki, S Koyama, R Hanada, RR Randolph, J Endo, T Kimura, K Ono
    Sci Rep, 2018;8(1):16749.
    Species: Rat
    Sample Types: Cell Culture Supernates
  38. Autophagy mediates tumor necrosis factor-?-induced phenotype switching in vascular smooth muscle A7r5 cell line
    Authors: M García-Mig, JA Riquelme, I Norambuena, PE Morales, F Sanhueza-O, C Nuñez-Soto, D Mondaca-Ru, N Cancino-Ar, A San Martín, M Chiong
    PLoS ONE, 2018;13(5):e0197210.
    Species: Rat
    Sample Types: Cell Culture Supernates
  39. Ablation of the Right Cardiac Vagus Nerve Reduces Acetylcholine Content without Changing the Inflammatory Response during Endotoxemia
    Authors: K Plaschke, TQM Do, F Uhle, T Brenner, MA Weigand, J Kopitz
    Int J Mol Sci, 2018;19(2):.
    Species: Rat
    Sample Types: Plasma
  40. The local cytokine and growth factor response to rhBMP-2 after spinal fusion
    Authors: JD Koerner, DZ Markova, GD Schroeder, BP Calio, A Shah, CW Brooks, AR Vaccaro, DG Anderson, CK Kepler
    Spine J, 2018;0(0):.
    Species: Rat
    Sample Types: Tissue Lysates
    Applications: ELISA (Standard)
  41. Short-term inhalation study of graphene oxide nanoplates
    Authors: YH Kim, MS Jo, JK Kim, JH Shin, JE Baek, HS Park, HJ An, JS Lee, BW Kim, HP Kim, KH Ahn, K Jeon, SM Oh, JH Lee, T Workman, EM Faustman, IJ Yu
    Nanotoxicology, 2018;0(0):1-15.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ELISA Capture
  42. Narrow Spectrum Kinase Inhibitors Demonstrate Promise for the Treatment of Dry Eye Disease and Other Ocular Inflammatory Disorders
    Authors: S Hagan, MCT Fyfe, B Ofori-Frim, K Oliver, MR Foster, S Sirohi, Y Solanke, M Doughty, A Rowley, M Taylor, S Webber, CA Walshe
    Invest. Ophthalmol. Vis. Sci., 2018;59(3):1443-1453.
    Species: Rat
    Sample Types: Tissue Homogenates
  43. Anti-inflammatory effect of Mongolian drug Naru-3 on traumatic spinal cord injury and its mechanism of action
    Authors: B Baiyila, B He, G He, T Long
    J. Int. Med. Res., 2018;0(0):3000605187601.
    Species: Rat
    Sample Types: Serum
  44. Cobra Venom Factor-induced complement depletion protects against lung ischemia reperfusion injury through alleviating blood-air barrier damage
    Authors: C Haihua, W Wei, H Kun, L Yuanli, L Fei
    Sci Rep, 2018;8(1):10346.
    Species: Rat
    Sample Types: BALF
  45. Sodium tanshinone IIA sulfonate improves inflammation, aortic endothelial cell apoptosis, disseminated intravascular coagulation and multiple organ damage in a rat heat stroke model
    Authors: F Chen, H Li, G Zhu, X Chen, Z Tang
    Mol Med Rep, 2017;0(0):.
    Species: Rat
    Sample Types: Serum
  46. Electroacupuncture serum inhibits TNF???mediated chondrocyte inflammation via the Ras?Raf?MEK1/2?ERK1/2 signaling pathway
    Authors: H Chen, X Shao, L Li, C Zheng, X Xu, X Hong, X Li, M Wu
    Mol Med Rep, 2017;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  47. Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model
    Authors: C Fanelli, SCA Arias, FG Machado, JK Okuma, DMAC Malheiros, H Azevedo, CA Moreira-Fi, NOS Camara, CK Fujihara, R Zatz
    Sci Rep, 2017;7(1):3192.
    Species: Rat
    Sample Types: Tissue Homogenates
  48. Oral Administration of Red Ginseng Extract Promotes Neurorestoration after Compressive Spinal Cord Injury in Rats
    Authors: P Zhu, K Samukawa, H Fujita, H Kato, M Sakanaka
    Evid Based Complement Alternat Med, 2017;2017(0):1265464.
    Species: Rat
    Sample Types: Tissue Homogenates
  49. Pharmacologic targeting ERK1/2 attenuates the development and progression of hyperuricemic nephropathy in rats
    Authors: N Liu, L Xu, Y Shi, L Fang, H Gu, H Wang, X Ding, S Zhuang
    Oncotarget, 2017;8(20):33807-33826.
    Species: Rat
    Sample Types: Tissue Culture Supernates
  50. Physical versus psychological social stress in male rats reveals distinct cardiovascular, inflammatory and behavioral consequences
    Authors: JE Finnell, CM Lombard, AR Padi, CM Moffitt, LB Wilson, CS Wood, SK Wood
    PLoS ONE, 2017;12(2):e0172868.
    Species: Rat
    Sample Types: Tissue Homogenates
  51. Neuroprotective effects of food restriction on autonomic innervation of the lacrimal gland in rat
    Authors: HZ Nooh, NH El-Saify, NMN Eldien
    Ann. Anat., 2017;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  52. Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system
    Authors: K Yan, Y Shen
    Mol Med Rep, 2017;0(0):.
    Species: Rat
    Sample Types: Serum
  53. Short-term treatment with taurolidine is associated with liver injury
    Authors: R Fahrner, A Möller, AT Press, A Kortgen, M Kiehntopf, F Rauchfuss, U Settmacher, AS Mosig
    BMC Pharmacol Toxicol, 2017;18(1):61.
    Species: Rat
    Sample Types: Serum
  54. Anti-aging effect and gene expression profiling of dung beetle glycosaminoglycan in aged rats
    Authors: MY Ahn, BJ Kim, HJ Kim, JS Hwang, YS Jung, KK Park
    Biomater Res, 2017;21(0):5.
    Species: Rat
    Sample Types: Serum
  55. Mori Folium water extract alleviates articular cartilage damages and inflammatory responses in monosodium iodoacetate?induced osteoarthritis rats
    Authors: JW Jeong, HH Lee, J Kim, EO Choi, H Hwang-Bo, HJ Kim, MY Kim, KI Ahn, GY Kim, KW Lee, KY Kim, SG Kim, SH Hong, C Park, HJ Cha, YH Choi
    Mol Med Rep, 2017;0(0):.
    Species: Rat
    Sample Types: Serum
  56. Investigation of the effect of phlomisoside F on complete Freund's adjuvant-induced arthritis
    Authors: X Zhang, Y Dong, H Dong, W Zhang, F Li
    Exp Ther Med, 2017;13(2):710-716.
    Species: Rat
    Sample Types: Serum
  57. Ferulaldehyde Improves the Effect of Methotrexate in Experimental Arthritis
    Authors: L Slovák, K Švík, D Mihalová, J Tóth, S Czigle, ? Pašková, F Bilka, K Bauerová
    Molecules, 2017;22(11):.
    Species: Rat
    Sample Types: Plasma
  58. The analgesic effect of rolipram is associated with the inhibition of the activation of the spinal astrocytic JNK/CCL2 pathway in bone cancer pain
    Authors: Chi-Hua Guo
    Int. J. Mol. Med, 2016;38(5):1433-1442.
    Species: Rat
    Sample Types: Tissue Homogenates
  59. Chronic Cognitive Dysfunction after Traumatic Brain Injury Is Improved with a Phosphodiesterase 4B Inhibitor
    J Neurosci, 2016;36(27):7095-108.
    Species: Rat
    Sample Types: Tissue Homogenates
  60. Spatiotemporal Cadence of Macrophage Polarisation in a Model of Light-Induced Retinal Degeneration.
    Authors: Jiao H, Natoli R, Valter K, Provis J, Rutar M
    PLoS ONE, 2015;10(12):e0143952.
    Species: Rat
    Sample Types: Tissue Homogenates
  61. Rhamnogalacturonan from Acmella oleracea (L.) R.K. Jansen: gastroprotective and ulcer healing properties in rats.
    Authors: Maria-Ferreira D, da Silva L, Mendes D, Cabrini D, Nascimento A, Iacomini M, Cipriani T, Santos A, Werner M, Baggio C
    PLoS ONE, 2014;9(1):e84762.
    Species: Rat
    Sample Types: Tissue Culture Supernates
  62. Catalpol ameliorates sodium taurocholate-induced acute pancreatitis in rats via inhibiting activation of nuclear factor kappa B.
    Authors: Xiao W, Yin G, Fan Y, Qiu L, Cang X, Yu G, Hu Y, Xing M, Wu D, Wang X, Hu G, Wan R
    Int J Mol Sci, 2014;15(7):11957-72.
    Species: Rat
    Sample Types: Serum
  63. Intrathecal antagonism of microglial TLR4 reduces inflammatory damage to blood-spinal cord barrier following ischemia/reperfusion injury in rats.
    Authors: Li X, Wang J, Fang B, Tan W, Ma H
    Mol Brain, 2014;7(0):28.
    Species: Rat
    Sample Types: Tissue Homogenates
  64. Epidermal growth factor receptor inhibitor ameliorates excessive astrogliosis and improves the regeneration microenvironment and functional recovery in adult rats following spinal cord injury.
    Authors: Li Z, Li J, Wang L, Zhang J, Wu J, Mao X, Shi G, Wang Q, Wang F, Zou J
    J Neuroinflammation, 2014;11(0):71.
    Species: Rat
    Sample Types: Cell Culture Supernates
  65. Atrial fibrosis in a chronic murine model of obstructive sleep apnea: mechanisms and prevention by mesenchymal stem cells.
    Authors: Ramos P, Rubies C, Torres M, Batlle M, Farre R, Brugada J, Montserrat J, Almendros I, Mont L
    Respir Res, 2014;15(0):54.
    Species: Rat
    Sample Types: Plasma
  66. Perioperative aspirin improves neurological outcome after focal brain ischemia possibly via inhibition of Notch 1 in rat.
    Authors: Wang Z, Huang W, Zuo Z
    J Neuroinflammation, 2014;11(0):56.
    Species: Rat
    Sample Types: Whole Tissue
  67. A short course of infusion of a hydrogen sulfide-donor attenuates endotoxemia induced organ injury via stimulation of anti-inflammatory pathways, with no additional protection from prolonged infusion.
    Cytokine, 2013;61(2):614-21.
    Species: Rat
    Sample Types: BALF
  68. Mechanical ventilation enhances HMGB1 expression in an LPS-induced lung injury model.
    Authors: Ding N, Wang F, Xiao H, Xu L, She S
    PLoS ONE, 2013;8(9):e74633.
    Species: Rat
    Sample Types: BALF
  69. Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
    J Neuroinflammation, 2012;9(0):181.
    Species: Rat
    Sample Types: Tissue Homogenates
  70. Identification of a serum-induced transcriptional signature associated with type 1 diabetes in the BioBreeding rat.
    Authors: Kaldunski M, Jia S, Geoffrey R, Basken J, Prosser S, Kansra S, Mordes JP, Lernmark A, Wang X, Hessner MJ
    Diabetes, 2010;59(10):2375-85.
    Species: Rat
    Sample Types: Cell Culture Supernates
  71. Evidence that intrathecal morphine-3-glucuronide may cause pain enhancement via toll-like receptor 4/MD-2 and interleukin-1beta.
    Authors: Lewis SS, Hutchinson MR, Rezvani N, Loram LC, Zhang Y, Maier SF, Rice KC, Watkins LR
    Neuroscience, 2010;165(2):569-83.
    Species: Rat
    Sample Types: CSF
  72. House dust mite induces direct airway inflammation in vivo: implications for future disease therapy?
    Authors: De Alba J, Raemdonck K, Dekkak A, Collins M, Wong S, Nials AT, Knowles RG, Belvisi MG, Birrell MA
    Eur. Respir. J., 2010;35(6):1377-87.
    Species: Rat
    Sample Types: BALF
  73. Neuregulin-ErbB signaling promotes microglial proliferation and chemotaxis contributing to microgliosis and pain after peripheral nerve injury.
    Authors: Calvo M, Zhu N, Tsantoulas C, Ma Z, Grist J, Loeb JA, Bennett DL
    J. Neurosci., 2010;30(15):5437-50.
    Species: Rat
    Sample Types: Cell Culture Supernates
  74. Spinal leptin contributes to the pathogenesis of neuropathic pain in rodents.
    Authors: Lim G, Wang S, Zhang Y, Tian Y, Mao J
    J. Clin. Invest., 2009;119(2):295-304.
    Species: Rat
    Sample Types: Cell Culture Supernates
  75. Peripheral osmotic stimulation inhibits the brain's innate immune response to microdialysis of acidic perfusion fluid adjacent to supraoptic nucleus.
    Authors: Summy-Long JY, Hu S
    Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009;297(5):R1532-45.
    Species: Rat
    Sample Types: Plasma
  76. Role of sensory innervation in the rat pulmonary neutrophil recruitment induced by staphylococcal enterotoxins type A and B.
    Authors: Desouza IA, Camargo EA, Mariano NS, Optiz-Neto JB, Resende JS, Mello GC, Costa SK, De Nucci G, Antunes E
    Eur. J. Pharmacol., 2009;613(1):128-34.
    Species: Rat
    Sample Types: BALF
  77. In vivo Angiotensin II AT1 receptor blockade selectively inhibits LPS-induced innate immune response and ACTH release in rat pituitary gland.
    Authors: Sanchez-Lemus E, Benicky J, Pavel J, Saavedra JM
    Brain Behav. Immun., 2009;23(7):945-57.
    Species: Rat
    Sample Types: Tissue Homogenates
  78. Role of central beta-adrenergic receptors in regulating proinflammatory cytokine responses to a peripheral bacterial challenge.
    Authors: Johnson JD, Cortez V, Kennedy SL, Foley TE, Hanson H, Fleshner M
    Brain Behav. Immun., 2008;22(7):1078-86.
    Species: Rat
    Sample Types: Plasma
  79. Substance P acts via the neurokinin receptor 1 to elicit bronchoconstriction, oxidative stress, and upregulated ICAM-1 expression after oil smoke exposure.
    Authors: Li PC, Chen WC, Chang LC, Lin SC
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008;294(5):L912-20.
    Species: Rat
    Sample Types: BALF
  80. Human interleukin-10 gene transfer is protective in a rat model of Parkinson's disease.
    Authors: Johnston LC, Su X, Maguire-Zeiss K, Horovitz K, Ankoudinova I, Guschin D, Hadaczek P, Federoff HJ, Bankiewicz K, Forsayeth J
    Mol. Ther., 2008;16(8):1392-9.
    Species: Rat
    Sample Types: Tissue Homogenates
  81. Cell-based angiopoietin-1 gene therapy for acute lung injury.
    Authors: McCarter SD, Mei SH, Lai PF, Zhang QW, Parker CH, Suen RS, Hood RD, Zhao YD, Deng Y, Han RN, Dumont DJ, Stewart DJ
    Am. J. Respir. Crit. Care Med., 2007;175(10):1014-26.
    Species: Rat
    Sample Types: BALF
  82. Prothymosin alpha lacking the nuclear localization signal as an effective gene therapeutic strategy in collagen-induced arthritis.
    Authors: Shiau AL, Chen SY, Chang MY
    J. Immunol., 2007;178(7):4688-94.
    Species: Rat
    Sample Types: Tissue Homogenates
  83. Cellular recruitment and cytokine generation in a rat model of allergic lung inflammation are differentially modulated by progesterone and estradiol.
    Authors: de Oliveira AP, Domingos HV, Cavriani G, Damazo AS, Dos Santos Franco AL, Oliani SM, Oliveira-Filho RM, Vargaftig BB, de Lima WT
    Am. J. Physiol., Cell Physiol., 2007;293(3):C1120-8.
    Species: Rat
    Sample Types: BALF
  84. Interleukin-6 mediates low-threshold mechanical allodynia induced by intrathecal HIV-1 envelope glycoprotein gp120.
    Authors: Schoeniger-Skinner DK, Ledeboer A, Frank MG, Milligan ED, Poole S, Martin D, Maier SF, Watkins LR
    Brain Behav. Immun., 2007;21(5):660-7.
    Species: Rat
    Sample Types: Tissue Homogenates
  85. IL-1beta promotes neurite outgrowth by deactivating RhoA via p38 MAPK pathway.
    Authors: Temporin K, Tanaka H, Kuroda Y, Okada K, Yachi K, Moritomo H, Murase T, Yoshikawa H
    Biochem. Biophys. Res. Commun., 2007;365(2):375-80.
    Species: Rat
    Sample Types: Tissue Homogenates
  86. Comprehensive gene expression profiling of rat lung reveals distinct acute and chronic responses to cigarette smoke inhalation.
    Authors: Stevenson CS, Docx C, Webster R, Battram C, Hynx D, Giddings J, Cooper PR, Chakravarty P, Rahman I, Marwick JA, Kirkham PA, Charman C, Richardson DL, Nirmala NR, Whittaker P, Butler K
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(5):L1183-93.
    Species: Rat
    Sample Types: BALF
  87. Changes of gene expression of iron regulatory proteins during turpentine oil-induced acute-phase response in the rat.
    Authors: Sheikh N, Dudas J, Ramadori G
    Lab. Invest., 2007;87(7):713-25.
    Species: Rat
    Sample Types: Serum
  88. Expression of the mu opioid receptor in the human immunodeficiency virus type 1 transgenic rat model.
    Authors: Chang SL, Beltran JA, Swarup S
    J. Virol., 2007;81(16):8406-11.
    Species: Rat
    Sample Types: Serum
  89. Gene transfer to interfere with TNFalpha signaling in neuropathic pain.
    Authors: Hao S, Mata M, Glorioso JC, Fink DJ
    Gene Ther., 2007;14(13):1010-6.
    Species: Rat
    Sample Types: Tissue Homogenates
  90. Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
    Authors: Barkho BZ, Song H, Aimone JB
    Stem Cells Dev., 2006;15(3):407-21.
    Species: Rat
    Sample Types: Cell Culture Supernates
  91. The age-related attenuation in long-term potentiation is associated with microglial activation.
    Authors: Griffin R, Nally R, Nolan Y, McCartney Y, Linden J, Lynch MA
    J. Neurochem., 2006;99(4):1263-72.
    Species: Rat
    Sample Types: Tissue Homogenates
  92. Interleukin-10 aerosol reduces proinflammatory mediators in bronchoalveolar fluid of endotoxemic rat.
    Authors: Hofstetter C, Flondor M, Hoegl S, Muhl H, Zwissler B
    Crit. Care Med., 2005;33(10):2317-22.
    Species: Rat
    Sample Types: BALF
  93. Prophylactic adenovirus-mediated human kallistatin gene therapy suppresses rat arthritis by inhibiting angiogenesis and inflammation.
    Authors: Wang CR, Chen SY, Wu CL, Liu MF, Jin YT, Chao L, Chao J
    Arthritis Rheum., 2005;52(4):1319-24.
    Species: Rat
    Sample Types: Tissue Homogenates
  94. Chlorpromazine and loxapine reduce interleukin-1beta and interleukin-2 release by rat mixed glial and microglial cell cultures.
    Authors: Labuzek K, Kowalski J, Gabryel B, Herman ZS
    Eur Neuropsychopharmacol, 2005;15(1):23-30.
    Species: Rat
    Sample Types: Cell Culture Supernates
  95. Cytokine profiles in the testes of rats treated with lipopolysaccharide reveal localized suppression of inflammatory responses.
    Authors: O&amp;amp;apos;Bryan MK
    Am. J. Physiol. Regul. Integr. Comp. Physiol., 2005;288(6):R1744-55.
    Species: Rat
    Sample Types: Tissue Homogenates
  96. Developmental changes induced by graded prenatal systemic hypoxic-ischemic insults in rats.
    Authors: Robinson S, Petelenz K, Li Q, Cohen ML, Dechant A, Tabrizi N, Bucek M, Lust D, Miller RH
    Neurobiol. Dis., 2005;18(3):568-81.
    Species: Rat
    Sample Types: Amniotic Fluid
  97. HIV-1 gp120 stimulates proinflammatory cytokine-mediated pain facilitation via activation of nitric oxide synthase-I (nNOS).
    Authors: Holguin A, O&amp;amp;apos;Connor KA, Biedenkapp J, Campisi J, Wieseler-Frank J, Milligan ED, Hansen MK, Spataro L, Maksimova E, Bravmann C, Martin D, Fleshner M, Maier SF, Watkins LR
    Pain, 2004;110(3):517-30.
    Species: Rat
    Sample Types: CSF
  98. Oral (gavage), in utero and post-natal exposure of Sprague-Dawley rats to low doses of tributyltin chloride. Part II: effects on the immune system.
    Authors: Tryphonas H, Cooke G, Caldwell D, Bondy G, Parenteau M, Hayward S, Pulido O
    Food Chem. Toxicol., 2004;42(2):221-35.
    Species: Rat
    Sample Types: Serum
  99. Gene transfer of tumor necrosis factor inhibitor improves the function of lung allografts.
    Authors: Tagawa T, Kozower BD, Kanaan SA, Daddi N, Muraoka M, Oka T, Ritter JH, Patterson GA
    J. Thorac. Cardiovasc. Surg., 2004;127(6):1558-63.
    Species: Rat
    Sample Types: Tissue Homogenates
  100. Differential cytokine response in interstitial fluid in skin and serum during experimental inflammation in rats.
    Authors: Nedrebo T, Reed RK, Jonsson R, Berg A, Wiig H
    J. Physiol. (Lond.), 2004;556(0):193-202.
    Species: Rat
    Sample Types: Interstitial Fluid
  101. Insulin attenuates the systemic inflammatory response in endotoxemic rats.
    Authors: Jeschke MG, Klein D, Bolder U, Einspanier R
    Endocrinology, 2004;145(9):4084-93.
    Species: Mouse
    Sample Types: Tissue Homogenates
  102. A method for measuring multiple cytokines from small samples.
    Authors: O&amp;amp;apos;Connor KA
    Brain Behav. Immun., 2004;18(3):274-80.
    Species: Rat
    Sample Types: CSF
  103. A role for proinflammatory cytokines and fractalkine in analgesia, tolerance, and subsequent pain facilitation induced by chronic intrathecal morphine.
    Authors: Johnston IN, Milligan ED, Wieseler-Frank J, Frank MG, Zapata V, Campisi J, Langer S, Martin D, Green P, Fleshner M, Leinwand L, Maier SF, Watkins LR
    J. Neurosci., 2004;24(33):7353-65.
    Species: Rat
    Sample Types: Tissue Homogenates
  104. Alveolar macrophage cytotoxicity for normal lung fibroblasts is mediated by nitric oxide release.
    Authors: Morgan DL, Shines CJ
    Toxicol In Vitro, 2004;18(1):139-46.
    Species: Rat
    Sample Types: Cell Culture Supernates
  105. Insulin treatment improves hepatic morphology and function through modulation of hepatic signals after severe trauma.
    Authors: Schubert T, Horch RE
    Ann. Surg., 2004;240(2):340-9.
    Species: Rat
    Sample Types: Tissue Homogenates
  106. Differential expression of cytokine genes and inducible nitric oxide synthase induced by opacity phenotype variants of Streptococcus pneumoniae during acute otitis media in the rat.
    Authors: Long JP, Tong HH, Shannon PA, DeMaria TF
    Infect. Immun., 2003;71(10):5531-40.
    Species: Rat
    Sample Types: Tissue Secretion
  107. Recipient intramuscular cotransfection of naked plasmid transforming growth factor beta1 and interleukin 10 ameliorates lung graft ischemia-reperfusion injury.
    Authors: D&amp;amp;apos;Ovidio F
    J. Thorac. Cardiovasc. Surg., 2002;124(2):259-69.
    Species: Rat
    Sample Types: BALF
  108. Role of IL-18 in acute lung inflammation.
    Authors: Jordan JA, Guo RF, Yun EC, Sarma V, Warner RL, Crouch LD, Senaldi G, Ulich TR, Ward PA
    J. Immunol., 2001;167(12):7060-8.
    Species: Rat
    Sample Types: BALF

FAQs

  1. Does this Quantikine ELISA Kit detect the pro form of the target protein?                        

    • This Quantikine ELISA kit is designed to detect both the natural and recombinant mature forms of the target protein.  The pro form of the target protein has not been evaluated in this assay.  To see the complete list of recombinant proteins tested for cross-reactivity or interference in this assay, please consult the "Specificity" section of the product insert. 

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Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit
By Anonymous on 08/11/2020
Application: Sample Tested: Serum,Tissue Homogenates

Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit
By Anonymous on 03/13/2020
Application: Sample Tested: Rat spinal cord

We tested IL-1β levels in homogenized rat spinal cord. The standard curve was excellent and the CV values were all very low. We have used kits from other companies and found RnD systems kits to be superior to all other companies. The kits from RnD systems result in much lower background levels and much lower CV levels, which really helps with consistency and repeatability. The image shows the standard curve and the green outlines the low CV values from a run containing homogenized rat spinal cord.


Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit
By Anonymous on 03/11/2019
Application: Sample Tested: Brain (cortex) tissue,Brain (hippocampus),Brain (hypothalamus) tissue,Brain tissue

One of the best ELISA kits for IL-1B measurement. Used it with brain homogenates and results are very reproducible. Before this kit, i worked with IL-1B ELISA kits from 2 other vendors, however i could find lot of cross- reactivity. Based on my excellent experience with RnD's mice IL-1B ELISA kits i tried rat kits and they did not disappoint me. I definitely recommend this kit for all your IL-1B related research.


Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit
By Anonymous on 05/08/2018
Application: Sample Tested: Heparin Plasma

Among best ELISAs I've worked with. Great consistency lot to lot, high sensitivity, easy to work with small amount of samples (50ul total, but samples can be diluted a number of times due to high sensitivity). Appropriate for concentration response curves. Linear standard curve for easy extrapolation of concentration values.


Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit, 2 Plate
By Anonymous on 02/26/2016
Application: Sample Tested: Rat Brain Tissue Homogenate