|Detection of Rat Neurexin 1 beta /NXRN1b by Western Blot. Western blot shows lysates of rat spinal cord tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Rat Neurexin 1 beta /NXRN1b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4524) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurexin 1 beta /NXRN1b at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
Neurexin 1 beta is an 80 kDa member of the Neurexin family of proteins. It is expressed on neuronal presynaptic membranes and contributes to synapse formation through binding to postsynaptic neuroligins. An alternate start site in the Neurexin 1 gene generate an alpha - (long) and beta - (short) isoform. Mature rat Neurexin 1 beta is a type I transmembrane glycoprotein that is 468 amino acids (aa) in length. It has a 346 aa extracellular region (aa 47‑392) that contains one laminin-like G domain (aa 91‑264). Within the G domain, the presence or absence of a splice site (aa 201‑230) materially impacts neuroligin binding. Over aa 47‑392, rat Neurexin 1 beta is 100% and 98% aa identical to mouse and human Neurexin 1 beta, respectively.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided