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Rat TNF-alpha DuoSet ELISA

Catalog #: DY510
97 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY510
DY510-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Rat TNF-alpha ELISA Standard Curve
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Product Details
Procedure
Citations (97)
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Supplemental Products
Reviews (4)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Rat TNF-alpha DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
62.5 - 4,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant rat TNF-alpha. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Rat TNF-alpha ELISA Standard Curve View Larger

Rat TNF-alpha ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily (1). It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (2-5). TNF-alpha is also involved in a number of pathological conditions including asthma, Crohn's disease, rheumatoid arthritis, neuropathic pain, obesity, type 2 diabetes, septic shock, autoimmunity, and cancer (5-11). 

Rat TNF-alpha is synthesized as a 26 kDa type II transmembrane protein that consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 156 aa extracellular domain (ECD) (12). Within the ECD, rat TNF-alpha shares 95% aa sequence identity with mouse, and 73% - 79% aa identity with bovine, canine, cotton rat, equine, feline, human, rhesus macaque, and porcine TNF-alpha. It is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (13). Cell surface TNF-alpha can both induce the lysis of tumor cells and virus infected cells, and generate its own downstream cell signaling following ligation by soluble TNF RI (14, 15). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (16-18). 
 TNF-alpha binds the ubiquitous 55 - 60 kDa TNF RI (19, 20) and the hematopoietic cell-restricted 78-80 kDa TNF RII (21, 22), both of which are also expressed as homotrimers (1, 23). Both type I and type II receptors bind TNF-alpha with comparable affinity and can promote NF kappa B activation (24-27). Only TNF RI, however, contains a cytoplasmic death domain which triggers the activation of apoptosis (3, 28). Soluble forms of both types of receptors are released into human serum and urine and can neutralize the biological activity of TNF-alpha (29-31).

Long Name:
Tumor Necrosis Factor alpha
Entrez Gene IDs:
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names:
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Rat TNF-alpha DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

97 Citations: Showing 1 - 10
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  1. Effect of citronellol on oxidative stress, neuroinflammation and autophagy pathways in an in vivo model of Parkinson's disease
    Authors: RL Jayaraj, S Azimullah, KA Parekh, SK Ojha, R Beiram
    Heliyon, 2022;8(11):e11434.
    Species: Rat
    Sample Types: Tissue Homogenates
  2. Effect of Hydrogel Containing Achyrocline satureioides (Asteraceae) Extract-Loaded Nanoemulsions on Wound Healing Activity
    Authors: LA Balestrin, PI Back, MDS Marques, GMS Araújo, MCF Carrasco, MM Batista, T Silveira, JL Rodrigues, FNS Fachel, LS Koester, VL Bassani, AP Horn, CL Dora, HF Teixeira
    Pharmaceutics, 2022;14(12):.
    Species: Rat
    Sample Types: Tissue Homogenates
  3. Triptolide Attenuates Muscular Inflammation and Oxidative Stress in a Delayed-Onset Muscle Soreness Animal Model
    Authors: CC Hsu, CC Tsai, PY Ko, TH Kwan, MY Liu, PT Wu, IM Jou
    International Journal of Environmental Research and Public Health, 2022;19(24):.
    Species: Rat
    Sample Types: Tissue Homogenates
  4. Cisplatin-induced changes in calcitonin gene-related peptide or TNF-alpha release in rat dorsal root ganglia in vitro model of neurotoxicity are not reverted by rosiglitazone
    Authors: HR Oliveira, MS Coelho, FAR Neves, DB Duarte
    Neurotoxicology, 2022;93(0):211-221.
    Species: Rat
    Sample Types: Cell Culture Supernates
  5. Low-Dose Aspirin Augments the Anti-Inflammatory Effects of Low-Dose Lithium in Lipopolysaccharide-Treated Rats
    Authors: R Shvartsur, G Agam, S Uzzan, AN Azab
    Pharmaceutics, 2022;14(5):.
    Species: Rat
    Sample Types: Tissue Homogenates
  6. Inhibitory Effects of Saururus chinensis Extract on Receptor for Advanced Glycation End-Products-Dependent Inflammation and Diabetes-Induced Dysregulation of Vasodilation
    Authors: K Hayashi, K Sato, S Ochi, S Kawano, S Munesue, A Harashima, Y Oshima, K Kimura, T Kyoi, Y Yamamoto
    International Journal of Molecular Sciences, 2022;23(10):.
    Species: Rat
    Sample Types: Plasma
  7. The superior beneficial effects of exercise training versus hormone replacement therapy on skeletal muscle of ovariectomized rats
    Authors: SB Silva, K Honorato-S, SP Costa, TE Domingues, TMM da Cruz, CM Rodrigues, KB Costa, JM Dos Santos, VK da Silva L, TP Gaiad, AP Santos, MF Dias-Peixo, CC Coimbra, AM Dos Reis, RE Szawka, PHS Figueiredo, HS Costa, MX Oliveira, VA Mendonça, ACR Lacerda
    Scientific Reports, 2022;12(1):8764.
    Species: Rat
    Sample Types: Tissue Homogenates
  8. Exposure to Nickel Oxide Nanoparticles Induces Acute and Chronic Inflammatory Responses in Rat Lungs and Perturbs the Lung Microbiome
    Authors: MJ Jeong, S Jeon, HS Yu, WS Cho, S Lee, D Kang, Y Kim, YJ Kim, SY Kim
    International Journal of Environmental Research and Public Health, 2022;19(1):.
    Species: Rat
    Sample Types: BALF
  9. LRRK2 Inhibition Mitigates the Neuroinflammation Caused by TLR2-Specific alpha-Synuclein and Alleviates Neuroinflammation-Derived Dopaminergic Neuronal Loss
    Authors: DH Ho, D Nam, M Seo, SW Park, W Seol, I Son
    Cells, 2022;11(5):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  10. Mast cells contribute to alveolar bone loss in Spontaneously Hypertensive Rats with periodontal disease regulating cytokines production
    Authors: VGB Brito, MS Patrocinio, MCL Sousa, AEA Barreto, SCT Frasnelli, VS Lara, CF Santos, SHP Oliveira
    PLoS ONE, 2021;16(3):e0247372.
    Species: Rat
    Sample Types: Tissue Homogenates
  11. In Vitro Evaluation of the Anti-Inflammatory Effect of KMUP-1 and In Vivo Analysis of Its Therapeutic Potential in Osteoarthritis
    Authors: SE Huang, E Sulistyowa, YY Chao, BN Wu, ZK Dai, JH Hsu, JL Yeh
    Biomedicines, 2021;9(6):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Inflammatory Cytokine Profile and Plasticity of Brain and Spinal Microglia in Response to ATP and Glutamate
    Authors: SJB Jesudasan, SJ Gupta, MA Churchward, KG Todd, IR Winship
    Frontiers in Cellular Neuroscience, 2021;15(0):634020.
    Species: Rat
    Sample Types: Cell Culture Supernates
  13. Small intestine remodeling in male Goto-Kakizaki rats
    Authors: JNB Pereira, GM Murata, FT Sato, AR Marosti, CRO Carvalho, R Curi
    Physiological Reports, 2021;9(3):e14755.
    Species: Rat
    Sample Types: Tissue Homogenates
  14. Programming of intestinal homeostasis in male rat offspring after maternal exposure to chlorpyrifos and/or to a high fat diet
    Authors: M Guibourden, H El Khayat, N Djekkoun, H Khorsi-Cau, V Bach, PM Anton, J Gay-Quéhei
    Scientific Reports, 2021;11(1):11420.
    Species: Rat
    Sample Types: Plasma
  15. Allanblackia floribunda Seed Extract Attenuates the Ethanol-Induced Gastric Ulcer in Rats via the Inhibition of TNF-&alpha and INF-&gamma Levels and Modulation in the Expression of Ki67 Protein
    Authors: FA Armah, IT Henneh, J Alake, W Ahlidja, B Amoani, EG Ofori, B Asante-Kye, GI Temitayo, CK Adokoh
    BioMed Research International, 2021;2021(0):6694572.
    Species: Rat
    Sample Types: Serum
  16. DEN-Induced Rat Model Reproduces Key Features of Human Hepatocellular Carcinoma
    Authors: K Kurma, O Manches, F Chuffart, N Sturm, K Gharzeddin, J Zhang, M Mercey-Res, S Rousseaux, A Millet, H Lerat, PN Marche, Z Macek Jilk, T Decaens
    Cancers, 2021;13(19):.
    Species: Rat
    Sample Types: Whole Tissue
  17. The Prophylactic Effects of Glutamine on Muscle Protein Synthesis and Degradation in Rats with Ethanol-Induced Liver Damage
    Authors: Q Xiao, YH Chen, SA Pratama, YL Chen, H Shirakawa, HC Peng, SC Yang
    Nutrients, 2021;13(8):.
    Species: Rat
    Sample Types: Tissue Homogenates
  18. TLR2- and TLR3-activated microglia induce different levels of neuronal network dysfunction in a context-dependent manner
    Authors: S Schilling, B Chausse, HO Dikmen, F Almouhanna, JO Hollnagel, A Lewen, O Kann
    Brain, Behavior, and Immunity, 2021;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  19. Neuron-Derived Extracellular Vesicles Modulate Microglia Activation and Function
    Authors: H Peng, BT Harvey, CI Richards, K Nixon
    Biology, 2021;10(10):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  20. Moderate Physical Exercise Activates ATR2 Receptors, Improving Inflammation and Oxidative Stress in the Duodenum of 2K1C Hypertensive Rats
    Authors: ACA da Silva, JS Severo, BLB Dos Santos, PHM Mendes, LMS Nobre, AP de Oliveir, FCS Ferreira, JVR Medeiros, RC Lima-Junio, A Havt, RC Palheta-Ju, AA Dos Santos, M Tolentino
    Frontiers in Physiology, 2021;12(0):734038.
    Species: Rat
    Sample Types: Tissue Homogenates
  21. Effect of Pulmonary Inflammation by Surface Functionalization of Zinc Oxide Nanoparticles
    Authors: A Jung, SH Kim, JY Yang, J Jeong, JK Lee, JH Oh, JH Lee
    Toxics, 2021;9(12):.
    Species: Rat
    Sample Types: BALF
  22. Therapeutic Effects of Fenofibrate Nano-Emulsion Eye Drops on Retinal Vascular Leakage and Neovascularization
    Authors: L Huang, W Liang, K Zhou, RA Wassel, ZD Ridge, JX Ma, B Wang
    Biology, 2021;10(12):.
    Species: Rat
    Sample Types: Tissue Homogenates
  23. Prenatal administration of IL-1Ra attenuate the neurodevelopmental impacts following non-pathogenic inflammation during pregnancy
    Authors: ME Brien, K Hughes, S Girard
    Scientific Reports, 2021;11(1):23404.
    Species: Rat
    Sample Types: Placenta
  24. The Poststroke Peripheral Immune Response Is Differentially Regulated by Leukemia Inhibitory Factor in Aged Male and Female Rodents
    Authors: SM Davis, LA Collier, SJ Messmer, KR Pennypacke
    Oxidative Medicine and Cellular Longevity, 2020;2020(0):8880244.
    Species: Rat
    Sample Types: Tissue Homogenates
  25. Acute sleep deprivation during pregnancy in rats: Rapid elevation of placental and fetal inflammation and kynurenic acid
    Authors: AM Baratta, NR Kanyuch, CA Cole, H Valafar, J Deslaurier, A Pocivavsek
    Neurobiol Stress, 2020;12(0):100204.
    Species: Rat
    Sample Types: Tissue Homogenates
  26. GM-CSF induces noninflammatory proliferation of microglia and disturbs electrical neuronal network rhythms in situ
    Authors: HO Dikmen, M Hemmerich, A Lewen, JO Hollnagel, B Chausse, O Kann
    J Neuroinflammation, 2020;17(1):235.
    Species: Rat
    Sample Types: Whole Tissue
  27. T cell infiltration and upregulation of MHCII in microglia leads to accelerated neuronal loss in an &alpha-synuclein rat model of Parkinson's disease
    Authors: MS Subbarayan, C Hudson, LD Moss, KR Nash, PC Bickford
    J Neuroinflammation, 2020;17(1):242.
    Species: Rat
    Sample Types: Whole Cells
  28. Hepcidin-to-Ferritin Ratio Is Decreased in Astrocytes With Extracellular Alpha-Synuclein and Iron Exposure
    Authors: J Cui, X Guo, Q Li, N Song, J Xie
    Front Cell Neurosci, 2020;14(0):47.
    Species: Rat
    Sample Types: Cell Culture Supernates
  29. &alpha-Bisabolol, a Dietary Bioactive Phytochemical Attenuates Dopaminergic Neurodegeneration through Modulation of Oxidative Stress, Neuroinflammation and Apoptosis in Rotenone-Induced Rat Model of Parkinson's disease
    Authors: H Javed, MFN Meeran, S Azimullah, L Bader Eddi, VD Dwivedi, NK Jha, S Ojha
    Biomolecules, 2020;10(10):.
    Species: Rat
    Sample Types: Tissue Homogenates
  30. Cilostazol ameliorates diabetic nephropathy by inhibiting highglucose- induced apoptosis
    Authors: CW Chian, YS Lee, YJ Lee, YH Chen, CP Wang, WC Lee, HJ Lee
    Korean J. Physiol. Pharmacol., 2020;24(5):403-412.
    Species: Rat
    Sample Types: Tissue Homogenates
  31. T cell infiltration and upregulation of MHCII in microglia leads to accelerated neuronal loss in an alpha-synuclein rat model of Parkinson's disease
    Authors: MS Subbarayan, C Hudson, LD Moss, KR Nash, PC Bickford
    J Neuroinflammation, 2020;17(1):242.
    Species: Rat
    Sample Types: Cell Culture Supernates
  32. Impacts of fish oil on the gut microbiota of rats with alcoholic liver damage
    Authors: YL Chen, H Shirakawa, NS Lu, HC Peng, Q Xiao, SC Yang
    J. Nutr. Biochem., 2020;86(0):108491.
    Species: Rat
    Sample Types: Cell Culture Supernates
  33. Polyphenols-Rich Fruit (Euterpe edulis Mart.) Prevents Peripheral Inflammatory Pathway Activation by the Short-Term High-Fat Diet
    Authors: AB Santamarin, G Jamar, LV Mennitti, DA Ribeiro, CM Cardoso, VV de Rosso, LM Oyama, LP Pisani
    Molecules, 2019;24(9):.
    Species: Rat
    Sample Types: Tissue Homogenates
  34. BRD4 inhibition attenuates inflammatory response in microglia and facilitates recovery after spinal cord injury in rats
    Authors: J Wang, J Chen, H Jin, D Lin, Y Chen, X Chen, B Wang, S Hu, Y Wu, Y Wu, Y Zhou, N Tian, W Gao, X Wang, X Zhang
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  35. Cholinergic Stimulation by Pyridostigmine Bromide Before Myocardial Infarction Prevent Cardiac and Autonomic Dysfunction
    Authors: CA Barboza, AR Fukushima, N Carrozzi, JF Machi, PMM Dourado, CT Mostarda, MC Irigoyen, L Nathanson, M Morris, EC Caperuto, B Rodrigues
    Sci Rep, 2019;9(1):2481.
    Species: Rat
    Sample Types: Plasma
  36. Exosomes derived from umbilical cord mesenchymal stem cells reduce microglia-mediated neuroinflammation in perinatal brain injury
    Authors: G Thomi, D Surbek, V Haesler, M Joerger-Me, A Schoeberle
    Stem Cell Res Ther, 2019;10(1):105.
    Species: Rat
    Sample Types: Tissue Homogenates
  37. Hyperbilirubinemia in Gunn Rats is Associated with Decreased Inflammatory Response in LPS-Mediated Systemic Inflammation
    Authors: P Valaskova, A Dvorak, M Lenicek, K Zizalova, N Kutinova-C, J Zelenka, M Cahova, L Vitek, L Muchova
    Int J Mol Sci, 2019;20(9):.
    Species: Rat
    Sample Types: Serum
  38. Time dependent dual effect of anti-inflammatory treatments on sarin-induced brain inflammation: suggested role of prostaglandins
    Authors: S Chapman, E Grauer, R Gez, I Egoz, S Lazar
    Neurotoxicology, 2019;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  39. Hepatoprotective Effect of Jianpi Huoxue Formula on Nonalcoholic Fatty Liver Disease Induced by Methionine-Choline-Deficient Diet in Rat
    Authors: Y Feng, Y Chen, B Yang, Q Lan, T Wang, G Cui, Z Ren, IC Choi, GP Leung, F Yan, D Chen, HH Yu, SMY Lee
    Biomed Res Int, 2019;2019(0):7465272.
    Species: Rat
    Sample Types: Serum
  40. The Preventive Effects of Greenshell Mussel (Perna canaliculus) on Early-Stage Metabolic Osteoarthritis in Rats with Diet-Induced Obesity
    Authors: P Siriarchav, MC Kruger, MR Miller, HS Tian, FM Wolber
    Nutrients, 2019;11(7):.
    Species: Rat
    Sample Types: Plasma
  41. Protective effect of Platymiscium floribundum Vog. in tree extract on periodontitis inflammation in rats
    Authors: JMO Freire, HV Chaves, AH Teixeira, LHT de Sousa, IR Pinto, JJDN Costa, NA de Sousa, KMA Pereira, VCC Girão, VCS Ferreira, JEÁ Dos Santos, MAS Lima, ATA Pimenta, RC Montenegro, MEA de Moraes, VPT Pinto, GC Filho, MM Bezerra
    PLoS ONE, 2019;14(11):e0223800.
    Species: Rat
    Sample Types: Gingivial Fluid
  42. TNF-&alpha mediated upregulation of NaV1.7 currents in rat dorsal root ganglion neurons is independent of CRMP2 SUMOylation
    Authors: FHP de Macedo, RD Aires, EG Fonseca, RCM Ferreira, DPD Machado, L Chen, FX Zhang, IA Souza, VS Lemos, TRL Romero, A Moutal, R Khanna, GW Zamponi, JS Cruz
    Mol Brain, 2019;12(1):117.
    Species: Rat
    Sample Types: Plasma
  43. A diet including xanthan gum triggers a pro-inflammatory response in Wistar rats inoculated with Walker 256 cells
    Authors: AB Silva Risc, NIP Neto, K Gascho, M Carnier, DA de Miranda, FP Silva, VT Boldarine, M Seelaender, EB Ribeiro, LM Oyama, CM Oller do N
    PLoS ONE, 2019;14(6):e0218567.
    Species: Rat
    Sample Types: Tissue Homogenates
  44. Improvement of diabetic wound healing by topical application of Vicenin-2 hydrocolloid film on Sprague Dawley rats
    Authors: WS Tan, P Arulselvan, SF Ng, CN Mat Taib, MN Sarian, S Fakurazi
    BMC Complement Altern Med, 2019;19(1):20.
    Species: Rat
    Sample Types: Tissue Homogenates
  45. Crude extract from Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz leaves decreased intra articular inflammation induced by zymosan in rats
    Authors: TR Falcão, CAO Rodrigues, AA de Araújo, CACX de Medeiro, LAL Soares, MRA Ferreira, RC Vasconcelo, RF de Araújo, MLD de Sousa L, GCB Guerra
    BMC Complement Altern Med, 2019;19(1):47.
    Species: Rat
    Sample Types: Synovial Fluid
  46. Cortical stimulation in conscious rats controls joint inflammation
    Authors: GS Bassi, L Ulloa, VR Santos, F Del Vecchi, P Delfino-Pe, GJ Rodrigues, JA Castania, FQ Cunha, HC Salgado, TM Cunha, N Garcia-Cai, A Kanashiro
    Prog. Neuropsychopharmacol. Biol. Psychiatry, 2018;0(0):.
    Species: Rat
    Sample Types: Synovial Fluid
  47. Early exposure to distinct sources of lipids affects differently the development and hepatic inflammatory profiles of 21-day-old rat offspring
    Authors: LV Mennitti, LM Oyama, AB Santamarin, CMDPO do Nascime, LP Pisani
    J Inflamm Res, 2018;11(0):11-24.
    Species: Rat
    Sample Types: Tissue Homogenates
  48. Characterization of the Myocardial Inflammatory Response in Acute Stress-Induced (Takotsubo) Cardiomyopathy
    Authors: HM Wilson, L Cheyne, PAJ Brown, K Kerr, A Hannah, J Srinivasan, N Duniak, G Horgan, DK Dawson
    JACC Basic Transl Sci, 2018;3(6):766-778.
    Species: Rat
    Sample Types: Serum
  49. Obesity and Type 2 Diabetes mellitus induce lipopolysaccharide tolerance in rat neutrophils
    Authors: WMT Kuwabara, CNF Yokota, R Curi, TC Alba-Loure
    Sci Rep, 2018;8(1):17534.
    Species: Rat
    Sample Types: BALF
  50. The Use of Ju�ara (Euterpe edulis Mart.) Supplementation for Suppression of NF-?B Pathway in the Hypothalamus after High-Fat Diet in Wistar Rats
    Authors: AB Santamarin, G Jamar, LV Mennitti, VV de Rosso, HC Cesar, LM Oyama, LP Pisani
    Molecules, 2018;23(7):.
    Species: Rat
    Sample Types: Tissue Homogenates
  51. Leukemia inhibitory factor modulates the peripheral immune response in a rat model of emergent large vessel occlusion
    Authors: SM Davis, LA Collier, ED Winford, CC Leonardo, CT Ajmo, EA Foran, TJ Kopper, JC Gensel, KR Pennypacke
    J Neuroinflammation, 2018;15(1):288.
    Species: Rat
    Sample Types: Tissue Homogenates
  52. Human Mesenchymal Stem Cell Secretome from Bone Marrow or Adipose-Derived Tissue Sources for Treatment of Hypoxia-Induced Pulmonary Epithelial Injury
    Authors: N Shologu, M Scully, JG Laffey, D O'Toole
    Int J Mol Sci, 2018;19(10):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  53. The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo
    Authors: SU Vay, LJ Flitsch, M Rabenstein, R Rogall, S Blaschke, J Kleinhaus, N Reinert, A Bach, GR Fink, M Schroeter, MA Rueger
    J Neuroinflammation, 2018;15(1):226.
    Species: Rat
    Sample Types: Cell Culture Supernates
  54. Activation of trigeminal ganglion satellite glial cells in CFA-induced tooth pulp pain in rats
    Authors: HF Filippini, PA Scalzilli, KM Costa, RDS Freitas, MM Campos
    PLoS ONE, 2018;13(11):e0207411.
    Species: Rat
    Sample Types: Serum
  55. Neuroprotection by Caffeine in Hyperoxia-Induced Neonatal Brain Injury
    Authors: S Endesfelde, U Weichelt, E Strauá, A Schl”r, M Sifringer, T Scheuer, C Bhrer, T Schmitz
    Int J Mol Sci, 2017;18(1):.
    Species: Rat
    Sample Types: Tissue Homogenates
  56. Different Dietary Proportions of Fish Oil Regulate Inflammatory Factors but Do Not Change Intestinal Tight Junction ZO-1 Expression in Ethanol-Fed Rats
    Authors: YW Chien, HC Peng, YL Chen, MH Pai, HY Wang, HL Chuang, SC Yang
    Mediators Inflamm., 2017;2017(0):5801768.
    Species: Rat
    Sample Types: Tissue Homogenates
  57. Impact of matrix metalloproteinase 9 rs3918242 genetic variant on lipid-lowering efficacy of simvastatin therapy in Chinese patients with coronary heart disease
    Authors: Y Xu, Y Wang, J Zhi, L Qi, T Zhang, X Li
    BMC Pharmacol Toxicol, 2017;18(1):28.
    Species: Human
    Sample Types: Plasma
  58. Food-grade TiO2 impairs intestinal and systemic immune homeostasis, initiates preneoplastic lesions and promotes aberrant crypt development in the rat colon
    Authors: S Bettini, E Boutet-Rob, C Cartier, C Com‚ra, E Gaultier, J Dupuy, N Naud, S Tach‚, P Grysan, S Reguer, N Thieriet, M R‚fr‚giers, D ThiaudiŠre, JP Cravedi, M CarriŠre, JN Audinot, FH Pierre, L Guzylack-P, E Houdeau
    Sci Rep, 2017;7(0):40373.
    Species: Rat
    Sample Types: Tissue Homogenates
  59. Incline treadmill exercise suppresses pain hypersensitivity associated with the modulation of pro-inflammatory cytokines and anti-inflammatory cytokine in rats with peripheral nerve injury
    Authors: KL Tsai, PC Huang, LK Wang, CH Hung, YW Chen
    Neurosci. Lett, 2017;643(0):27-31.
    Species: Rat
    Sample Types: Tissue Homogenates
  60. Post-translational Activation of Glutamate Cysteine Ligase with Dimercaprol: A Novel Mechanism of Inhibiting Neuroinflammation in vitro
    Authors: PB McElroy, A Sri Hari, BJ Day, M Patel
    J. Biol. Chem, 2017;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  61. Carotid sinus nerve electrical stimulation in conscious rats attenuates systemic inflammation via chemoreceptor activation
    Authors: FM Santos-Alm, G Domingos-S, CA Meschiari, LC Fávaro, C Becari, JA Castania, A Lopes, TM Cunha, DJA Moraes, FQ Cunha, L Ulloa, A Kanashiro, GCSV Tezini, HC Salgado
    Sci Rep, 2017;7(1):6265.
    Species: Rat
    Sample Types: Plasma
  62. Intestinal anti-inflammatory activity of Ground Cherry (Physalis angulata L.) standardized CO2 phytopharmaceutical preparation
    Authors: LD Almeida Ju, AEV Quaglio, CAR de Almeida, LC Di Stasi
    World J. Gastroenterol., 2017;23(24):4369-4380.
    Species: Rat
    Sample Types: Tissue Homogenates
  63. Original research paper. Pulmonary prophylactic impact of melatonin and/or quercetin: A novel therapy for inflammatory hypoxic stress in rats
    Authors: NM Al-Rasheed, L Fadda, HA Attia, IA Sharaf, AM Mohamed, NM Al-Rasheed
    Acta Pharm, 2017;67(1):125-135.
    Species: Rat
    Sample Types: Serum
  64. Propentofylline Prevents Sickness Behavior and Depressive-Like Behavior Induced by Lipopolysaccharide in Rats via Neuroinflammatory Pathway
    Authors: MM Moraes, MC Galv?o, D Cabral, CP Coelho, N Queiroz-Ha, MF Martins, EF Bondan, MM Bernardi, TB Kirsten
    PLoS ONE, 2017;12(1):e0169446.
    Species: Rat
    Sample Types: Plasma
  65. Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon
    Authors: RR Oliveira, R Medina de, L Magalh?es, F Guimar?es, F Tovar-Moll, L Eurico Nas, GA de Castro
    PLoS ONE, 2017;12(1):e0169513.
    Species: Rat
    Sample Types: Tissue Homogenates
  66. Impact of gestational nicotine exposure on intrauterine and fetal infection in a rodent model
    Authors: M von Chamie, L Reyes, LF Hayward, MB Brown
    Biol. Reprod., 2017;96(5):1071-1084.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: ELISA Capture
  67. The protective role of nitric oxide-dependent innate immunosuppression in the early stage of cartilage damage in rats: Role of nitric oxide in cartilage damage
    Authors: CC Hsu, CL Lin, IM Jou, PH Wang, JS Lee
    Bone Joint Res, 2017;6(4):253-258.
    Species: Rat
    Sample Types: Tissue Homogenates
  68. Pyrrolidine dithiocarbamate administered during ex-vivo lung perfusion promotes rehabilitation of injured donor rat lungs obtained after prolonged warm ischemia
    Authors: C Francioli, X Wang, R Parapanov, E Abdelnour, J Lugrin, F Gronchi, J Perentes, P Eckert, HB Ris, L Piquilloud, T Krueger, L Liaudet
    PLoS ONE, 2017;12(3):e0173916.
    Species: Rat
    Sample Types: BALF
  69. Epigallocatechin-3-gallate prevents cardiac apoptosis by modulating the intrinsic apoptotic pathway in isoproterenol-induced myocardial infarction
    Authors: Mohammed Dardor
    Eur. J. Pharmacol., 2016;0(0):.
    Species: Rat
    Sample Types: Serum
  70. The Involvement of Pial Microvessels in Leukocyte Invasion after Mild Traumatic Brain Injury
    PLoS ONE, 2016;11(12):e0167677.
    Species: Rat
    Sample Types: Tissue Homogenates
  71. Combination of methylprednisolone and rosiglitazone promotes recovery of neurological function after spinal cord injury
    Neural Regen Res, 2016;11(10):1678-1684.
    Species: Rat
    Sample Types: Tissue Homogenates
  72. Intra-articular blockade of P2X7 receptor reduces the articular hyperalgesia and inflammation in the knee joint synovitis especially in female rats
    Authors: Cláudia Herrera Tambeli
    J Pain, 2016;0(0):.
    Species: Rat
    Sample Types: Synovial Lavage Fluid
  73. Impact of exercise training associated to pyridostigmine treatment on autonomic function and inflammatory profile after myocardial infarction in rats
    Authors: Bruno Rodrigues
    Int. J. Cardiol., 2016;0(0):.
    Species: Rat
    Sample Types: Plasma
  74. Muscle hyperalgesia induced by peripheral P2X3 receptors is modulated by inflammatory mediators.
    Authors: Schiavuzzo J, Teixeira J, Melo B, da Silva dos Santos D, Jorge C, Oliveira-Fusaro M, Parada C
    Neuroscience, 2015;285(0):24-33.
    Species: Rat
    Sample Types: Tissue Homogenates
  75. Zinc prevents sickness behavior induced by lipopolysaccharides after a stress challenge in rats.
    Authors: Kirsten T, Galvao M, Reis-Silva T, Queiroz-Hazarbassanov N, Bernardi M
    PLoS ONE, 2015;10(3):e0120263.
    Species: Rat
    Sample Types: Plasma
  76. Exploring LPS-induced sepsis in rats and mice as a model to study potential protective effects of the nociceptin/orphanin FQ system
    Authors: Jonathan P Thompson
    Peptides, 2014;61(0):56-60.
    Species: Rat
    Sample Types: Serum
  77. S-nitrosoglutathione accelerates recovery from 5-fluorouracil-induced oral mucositis.
    Authors: Skeff M, Brito G, de Oliveira M, Braga C, Cavalcante M, Baldim V, Holanda-Afonso R, Silva-Boghossian C, Colombo A, Ribeiro R, Moura-Neto V, Leitao R
    PLoS ONE, 2014;9(12):e113378.
    Species: Hamster
    Sample Types: Tissue Homogenates
  78. Oligofructose supplementation (10%) during pregnancy and lactation does not change the inflammatory effect of concurrent trans fatty acid ingestion on 21-day-old offspring.
    Authors: Hachul A, Mennitti L, de Oliveira J, Moreno M, Okuda M, Dos Santos B, Oyama L, Ribeiro E, do Nascimento C, Pisani L
    Lipids Health Dis, 2013;12(0):59.
    Species: Rat
    Sample Types: Tissue Homogenates
  79. Protective effect of hypercapnic acidosis in ischemia-reperfusion lung injury is attributable to upregulation of heme oxygenase-1.
    Authors: Wu S, Li M, Ko F, Wu G, Huang K, Chu S
    PLoS ONE, 2013;8(9):e74742.
    Species: Rat
    Sample Types: BALF
  80. Effects of freshwater clam extract supplementation on time to exhaustion, muscle damage, pro/anti-inflammatory cytokines, and liver injury in rats after exhaustive exercise.
    Authors: Huang K, Wu W, Yang F, Chiu Y, Peng T, Hsu B, Liao K, Lee R
    Molecules, 2013;18(4):3825-38.
    Species: Rat
    Sample Types: Plasma
  81. Depot-specific modulation of adipokine levels in rat adipose tissue by diet-induced obesity: the effect of aerobic training and energy restriction.
    Authors: Yamashita AS, Lira FS, Rosa JC, Paulino EC, Brum PC, Negrao CE, dos Santos RV, Batista ML, do Nascimento CO, Oyama LM, Seelaender M
    Cytokine, 2010;52(3):168-74.
    Species: Rat
    Sample Types: Tissue Homogenates
  82. The lectin-like domain of tumor necrosis factor improves lung function after rat lung transplantation--potential role for a reduction in reactive oxygen species generation.
    Authors: Hamacher J, Stammberger U, Roux J, Kumar S, Yang G, Xiong C, Schmid RA, Fakin RM, Chakraborty T, Hossain HM, Pittet JF, Wendel A, Black SM, Lucas R
    Crit. Care Med., 2010;38(3):871-8.
    Species: Rat
    Sample Types: BALF
  83. Supernatant of stored platelets causes lung inflammation and coagulopathy in a novel in vivo transfusion model.
    Authors: Vlaar AP, Hofstra JJ, Kulik W
    Blood, 2010;116(8):1360-8.
    Species: Rat
    Sample Types: Tissue Homogenates
  84. TNF-alpha, IL-1beta, IL-6, and cinc-1 levels in rat brain after meningitis induced by Streptococcus pneumoniae.
    Authors: Barichello T, dos Santos I, Savi GD, Simoes LR, Silvestre T, Comim CM, Sachs D, Teixeira MM, Teixeira AL, Quevedo J
    J. Neuroimmunol., 2010;221(1):42-5.
    Species: Rat
    Sample Types: Tissue Homogenates
  85. Effects of ciliary muscle plasmid electrotransfer of TNF-alpha soluble receptor variants in experimental uveitis.
    Authors: Touchard E, Bloquel C, Bigey P, Kowalczuc L, Jonet L, Thillaye-Goldenberg B, Naud MC, Scherman D, de Kozak Y, Benezra D, Behar-Cohen F
    Gene Ther., 2009;16(7):862-73.
    Species: Rat
    Sample Types: Aqueous Humor
  86. Augmentation of endogenous cannabinoid tone modulates lipopolysaccharide-induced alterations in circulating cytokine levels in rats.
    Authors: Roche M, Kelly JP, O'Driscoll M, O'Driscoll M, Finn DP
    Immunology, 2008;125(2):263-71.
  87. MAPKs are key players in mediating cytokine release and cell death induced by unconjugated bilirubin in cultured rat cortical astrocytes.
    Authors: Fernandes A, Falcao AS, Silva RF, Brito MA, Brites D
    Eur. J. Neurosci., 2007;25(4):1058-68.
    Species: Rat
    Sample Types: Cell Culture Supernates
  88. Sirtuin regulates cigarette smoke-induced proinflammatory mediator release via RelA/p65 NF-kappaB in macrophages in vitro and in rat lungs in vivo: implications for chronic inflammation and aging.
    Authors: Yang SR, Wright J, Bauter M, Seweryniak K, Kode A, Rahman I
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(2):L567-76.
    Species: Rat
    Sample Types: BALF
  89. Utility of exhaled nitric oxide as a noninvasive biomarker of lung inflammation in a disease model.
    Authors: Birrell MA, McCluskie K, Hardaker E, Knowles R, Belvisi MG
    Eur. Respir. J., 2006;28(6):1236-44.
    Species: Rat
    Sample Types: BALF
  90. Cardiomyocyte function after burn injury and lipopolysaccharide exposure: single-cell contraction analysis and cytokine secretion profile.
    Authors: Niederbichler AD, Westfall MV, Su GL, Donnerberg J, Usman A, Vogt PM, Ipaktchi KR, Arbabi S, Wang SC, Hemmila MR
    Shock, 2006;25(2):176-83.
    Species: Rat
    Sample Types: Cell Culture Supernates
  91. Role of matrix metalloproteinases in the inflammatory response in human airway cell-based assays and in rodent models of airway disease.
    Authors: Birrell MA, Wong S, Dekkak A, De Alba J, Haj-Yahia S, Belvisi MG
    J. Pharmacol. Exp. Ther., 2006;318(2):741-50.
    Species: Rat
    Sample Types: BALF
  92. Inflammatory signalling pathways involved in astroglial activation by unconjugated bilirubin.
    Authors: Fernandes A, Falcao AS, Silva RF, Gordo AC, Gama MJ, Brito MA, Brites D
    J. Neurochem., 2006;96(6):1667-79.
    Species: Rat
    Sample Types: Cell Culture Supernates
  93. Effects of estradiol on lipopolysaccharide and Pam3Cys stimulation of CCL20/macrophage inflammatory protein 3 alpha and tumor necrosis factor alpha production by uterine epithelial cells in culture.
    Authors: Crane-Godreau MA, Wira CR
    Infect. Immun., 2005;73(7):4231-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  94. GM-CSF action in the CNS decreases food intake and body weight.
    Authors: Reed JA, Clegg DJ, Smith KB, Tolod-Richer EG, Matter EK, Picard LS, Seeley RJ
    J. Clin. Invest., 2005;115(11):3035-44.
    Species: Rat
    Sample Types: Serum
  95. CCL20/macrophage inflammatory protein 3alpha and tumor necrosis factor alpha production by primary uterine epithelial cells in response to treatment with lipopolysaccharide or Pam3Cys.
    Authors: Crane-Godreau MA, Wira CR
    Infect. Immun., 2005;73(1):476-84.
    Species: Rat
    Sample Types: Cell Culture Supernates
  96. Maternal poly I:C exposure during pregnancy regulates TNF alpha, BDNF, and NGF expression in neonatal brain and the maternal-fetal unit of the rat.
    Authors: Gilmore JH, Jarskog LF, Vadlamudi S
    J. Neuroimmunol., 2004;159(1):106-12.
    Species: Rat
    Sample Types: Tissue Homogenates
  97. A study of the biologic activity of trauma-hemorrhagic shock mesenteric lymph over time and the relative role of cytokines.
    Authors: Davidson MT, Deitch EA, Lu Q, Osband A, Feketeova E, Nemeth ZH, Hasko G, Xu DZ
    Surgery, 2004;136(1):32-41.
    Species: Rat
    Sample Types: Lymph

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Rat TNF-alpha DuoSet ELISA
By Anonymous on 08/16/2022
Sample Tested: Peritoneal macrophages

Lower STD shows less difference

Rat TNF-alpha DuoSet ELISA DY510
Rat TNF-alpha DuoSet ELISA
By Shekhar Yeshwante on 08/11/2020
Sample Tested: bronchoalveolar lavage fluid,Serum,Tissue Homogenates
Rat TNF-alpha DuoSet ELISA DY510
Rat TNF-alpha DuoSet ELISA
By Vinitha/Chong Ahn Thiyagarajan Upaassana on 12/28/2017
Sample Tested: Plasma
Rat TNF-alpha DuoSet ELISA DY510
Rat TNF-alpha DuoSet ELISA
By Anonymous on 10/06/2016
Sample Tested: Cell culture supernatant
Rat TNF-alpha DuoSet ELISA DY510