Rat TNF-alpha DuoSet ELISA

Catalog # Availability Size / Price Qty
DY510
DY510-05
Ancillary Products Available
Rat TNF-alpha ELISA Standard Curve
1 Image
Product Details
Procedure
Citations (57)
FAQs
Supplemental Products
Reviews (2)

Rat TNF-alpha DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant rat TNF-alpha. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Data Example

Rat TNF-alpha ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TNF-alpha

Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.

Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

Long Name:
Tumor Necrosis Factor alpha
Entrez Gene IDs:
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names:
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Rat TNF-alpha DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

57 Citations: Showing 1 - 10
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  1. Polyphenols-Rich Fruit (Euterpe edulis Mart.) Prevents Peripheral Inflammatory Pathway Activation by the Short-Term High-Fat Diet
    Authors: AB Santamarin, G Jamar, LV Mennitti, DA Ribeiro, CM Cardoso, VV de Rosso, LM Oyama, LP Pisani
    Molecules, 2019;24(9):.
    Species: Rat
    Sample Types: Tissue Homogenates
  2. Time dependent dual effect of anti-inflammatory treatments on sarin-induced brain inflammation: suggested role of prostaglandins
    Authors: S Chapman, E Grauer, R Gez, I Egoz, S Lazar
    Neurotoxicology, 2019;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  3. Hyperbilirubinemia in Gunn Rats is Associated with Decreased Inflammatory Response in LPS-Mediated Systemic Inflammation
    Authors: P Valaskova, A Dvorak, M Lenicek, K Zizalova, N Kutinova-C, J Zelenka, M Cahova, L Vitek, L Muchova
    Int J Mol Sci, 2019;20(9):.
    Species: Rat
    Sample Types: Serum
  4. Exosomes derived from umbilical cord mesenchymal stem cells reduce microglia-mediated neuroinflammation in perinatal brain injury
    Authors: G Thomi, D Surbek, V Haesler, M Joerger-Me, A Schoeberle
    Stem Cell Res Ther, 2019;10(1):105.
    Species: Rat
    Sample Types: Tissue Homogenates
  5. Cholinergic Stimulation by Pyridostigmine Bromide Before Myocardial Infarction Prevent Cardiac and Autonomic Dysfunction
    Authors: CA Barboza, AR Fukushima, N Carrozzi, JF Machi, PMM Dourado, CT Mostarda, MC Irigoyen, L Nathanson, M Morris, EC Caperuto, B Rodrigues
    Sci Rep, 2019;9(1):2481.
    Species: Rat
    Sample Types: Plasma
  6. BRD4 inhibition attenuates inflammatory response in microglia and facilitates recovery after spinal cord injury in rats
    Authors: J Wang, J Chen, H Jin, D Lin, Y Chen, X Chen, B Wang, S Hu, Y Wu, Y Wu, Y Zhou, N Tian, W Gao, X Wang, X Zhang
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  7. Crude extract from Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz leaves decreased intra articular inflammation induced by zymosan in rats
    Authors: TR Falcão, CAO Rodrigues, AA de Araújo, CACX de Medeiro, LAL Soares, MRA Ferreira, RC Vasconcelo, RF de Araújo, MLD de Sousa L, GCB Guerra
    BMC Complement Altern Med, 2019;19(1):47.
    Species: Rat
    Sample Types: Synovial Fluid
  8. Improvement of diabetic wound healing by topical application of Vicenin-2 hydrocolloid film on Sprague Dawley rats
    Authors: WS Tan, P Arulselvan, SF Ng, CN Mat Taib, MN Sarian, S Fakurazi
    BMC Complement Altern Med, 2019;19(1):20.
    Species: Rat
    Sample Types: Tissue Homogenates
  9. Activation of trigeminal ganglion satellite glial cells in CFA-induced tooth pulp pain in rats
    Authors: HF Filippini, PA Scalzilli, KM Costa, RDS Freitas, MM Campos
    PLoS ONE, 2018;13(11):e0207411.
    Species: Rat
    Sample Types: Serum
  10. The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo
    Authors: SU Vay, LJ Flitsch, M Rabenstein, R Rogall, S Blaschke, J Kleinhaus, N Reinert, A Bach, GR Fink, M Schroeter, MA Rueger
    J Neuroinflammation, 2018;15(1):226.
    Species: Rat
    Sample Types: Cell Culture Supernates
  11. Human Mesenchymal Stem Cell Secretome from Bone Marrow or Adipose-Derived Tissue Sources for Treatment of Hypoxia-Induced Pulmonary Epithelial Injury
    Authors: N Shologu, M Scully, JG Laffey, D O'Toole
    Int J Mol Sci, 2018;19(10):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  12. Leukemia inhibitory factor modulates the peripheral immune response in a rat model of emergent large vessel occlusion
    Authors: SM Davis, LA Collier, ED Winford, CC Leonardo, CT Ajmo, EA Foran, TJ Kopper, JC Gensel, KR Pennypacke
    J Neuroinflammation, 2018;15(1):288.
    Species: Rat
    Sample Types: Tissue Homogenates
  13. The Use of Ju�ara (Euterpe edulis Mart.) Supplementation for Suppression of NF-?B Pathway in the Hypothalamus after High-Fat Diet in Wistar Rats
    Authors: AB Santamarin, G Jamar, LV Mennitti, VV de Rosso, HC Cesar, LM Oyama, LP Pisani
    Molecules, 2018;23(7):.
    Species: Rat
    Sample Types: Tissue Homogenates
  14. Obesity and Type 2 Diabetes mellitus induce lipopolysaccharide tolerance in rat neutrophils
    Authors: WMT Kuwabara, CNF Yokota, R Curi, TC Alba-Loure
    Sci Rep, 2018;8(1):17534.
    Species: Rat
    Sample Types: BALF
  15. Characterization of the Myocardial Inflammatory Response in Acute Stress-Induced (Takotsubo) Cardiomyopathy
    Authors: HM Wilson, L Cheyne, PAJ Brown, K Kerr, A Hannah, J Srinivasan, N Duniak, G Horgan, DK Dawson
    JACC Basic Transl Sci, 2018;3(6):766-778.
    Species: Rat
    Sample Types: Serum
  16. The protective role of nitric oxide-dependent innate immunosuppression in the early stage of cartilage damage in rats: Role of nitric oxide in cartilage damage
    Authors: CC Hsu, CL Lin, IM Jou, PH Wang, JS Lee
    Bone Joint Res, 2017;6(4):253-258.
    Species: Rat
    Sample Types: Tissue Homogenates
  17. Neuroprotection by Caffeine in Hyperoxia-Induced Neonatal Brain Injury
    Authors: S Endesfelde, U Weichelt, E Strauá, A Schl”r, M Sifringer, T Scheuer, C Bhrer, T Schmitz
    Int J Mol Sci, 2017;18(1):.
    Species: Rat
    Sample Types: Tissue Homogenates
  18. Pyrrolidine dithiocarbamate administered during ex-vivo lung perfusion promotes rehabilitation of injured donor rat lungs obtained after prolonged warm ischemia
    Authors: C Francioli, X Wang, R Parapanov, E Abdelnour, J Lugrin, F Gronchi, J Perentes, P Eckert, HB Ris, L Piquilloud, T Krueger, L Liaudet
    PLoS ONE, 2017;12(3):e0173916.
    Species: Rat
    Sample Types: BALF
  19. Post-translational Activation of Glutamate Cysteine Ligase with Dimercaprol: A Novel Mechanism of Inhibiting Neuroinflammation in vitro
    Authors: PB McElroy, A Sri Hari, BJ Day, M Patel
    J. Biol. Chem, 2017;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  20. Incline treadmill exercise suppresses pain hypersensitivity associated with the modulation of pro-inflammatory cytokines and anti-inflammatory cytokine in rats with peripheral nerve injury
    Authors: KL Tsai, PC Huang, LK Wang, CH Hung, YW Chen
    Neurosci. Lett, 2017;643(0):27-31.
    Species: Rat
    Sample Types: Tissue Homogenates
  21. Food-grade TiO2 impairs intestinal and systemic immune homeostasis, initiates preneoplastic lesions and promotes aberrant crypt development in the rat colon
    Authors: S Bettini, E Boutet-Rob, C Cartier, C Com‚ra, E Gaultier, J Dupuy, N Naud, S Tach‚, P Grysan, S Reguer, N Thieriet, M R‚fr‚giers, D ThiaudiŠre, JP Cravedi, M CarriŠre, JN Audinot, FH Pierre, L Guzylack-P, E Houdeau
    Sci Rep, 2017;7(0):40373.
    Species: Rat
    Sample Types: Tissue Homogenates
  22. Original research paper. Pulmonary prophylactic impact of melatonin and/or quercetin: A novel therapy for inflammatory hypoxic stress in rats
    Authors: NM Al-Rasheed, L Fadda, HA Attia, IA Sharaf, AM Mohamed, NM Al-Rasheed
    Acta Pharm, 2017;67(1):125-135.
    Species: Rat
    Sample Types: Serum
  23. Different Dietary Proportions of Fish Oil Regulate Inflammatory Factors but Do Not Change Intestinal Tight Junction ZO-1 Expression in Ethanol-Fed Rats
    Authors: YW Chien, HC Peng, YL Chen, MH Pai, HY Wang, HL Chuang, SC Yang
    Mediators Inflamm., 2017;2017(0):5801768.
    Species: Rat
    Sample Types: Tissue Homogenates
  24. Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon
    Authors: RR Oliveira, R Medina de, L Magalh?es, F Guimar?es, F Tovar-Moll, L Eurico Nas, GA de Castro
    PLoS ONE, 2017;12(1):e0169513.
    Species: Rat
    Sample Types: Tissue Homogenates
  25. Propentofylline Prevents Sickness Behavior and Depressive-Like Behavior Induced by Lipopolysaccharide in Rats via Neuroinflammatory Pathway
    Authors: MM Moraes, MC Galv?o, D Cabral, CP Coelho, N Queiroz-Ha, MF Martins, EF Bondan, MM Bernardi, TB Kirsten
    PLoS ONE, 2017;12(1):e0169446.
    Species: Rat
    Sample Types: Plasma
  26. Impact of matrix metalloproteinase 9 rs3918242 genetic variant on lipid-lowering efficacy of simvastatin therapy in Chinese patients with coronary heart disease
    Authors: Y Xu, Y Wang, J Zhi, L Qi, T Zhang, X Li
    BMC Pharmacol Toxicol, 2017;18(1):28.
    Species: Human
    Sample Types: Plasma
  27. Carotid sinus nerve electrical stimulation in conscious rats attenuates systemic inflammation via chemoreceptor activation
    Authors: FM Santos-Alm, G Domingos-S, CA Meschiari, LC Fávaro, C Becari, JA Castania, A Lopes, TM Cunha, DJA Moraes, FQ Cunha, L Ulloa, A Kanashiro, GCSV Tezini, HC Salgado
    Sci Rep, 2017;7(1):6265.
    Species: Rat
    Sample Types: Plasma
  28. Intestinal anti-inflammatory activity of Ground Cherry (Physalis angulata L.) standardized CO2 phytopharmaceutical preparation
    Authors: LD Almeida Ju, AEV Quaglio, CAR de Almeida, LC Di Stasi
    World J. Gastroenterol., 2017;23(24):4369-4380.
    Species: Rat
    Sample Types: Tissue Homogenates
  29. Combination of methylprednisolone and rosiglitazone promotes recovery of neurological function after spinal cord injury
    Neural Regen Res, 2016;11(10):1678-1684.
    Species: Rat
    Sample Types: Tissue Homogenates
  30. Epigallocatechin-3-gallate prevents cardiac apoptosis by modulating the intrinsic apoptotic pathway in isoproterenol-induced myocardial infarction
    Authors: Mohammed Dardor
    Eur. J. Pharmacol., 2016;0(0):.
    Species: Rat
    Sample Types: Serum
  31. Intra-articular blockade of P2X7 receptor reduces the articular hyperalgesia and inflammation in the knee joint synovitis especially in female rats
    Authors: Cláudia Herrera Tambeli
    J Pain, 2016;0(0):.
    Species: Rat
    Sample Types: Synovial Lavage Fluid
  32. The Involvement of Pial Microvessels in Leukocyte Invasion after Mild Traumatic Brain Injury
    PLoS ONE, 2016;11(12):e0167677.
    Species: Rat
    Sample Types: Tissue Homogenates
  33. Impact of exercise training associated to pyridostigmine treatment on autonomic function and inflammatory profile after myocardial infarction in rats
    Authors: Bruno Rodrigues
    Int. J. Cardiol., 2016;0(0):.
    Species: Rat
    Sample Types: Plasma
  34. Muscle hyperalgesia induced by peripheral P2X3 receptors is modulated by inflammatory mediators.
    Authors: Schiavuzzo J, Teixeira J, Melo B, da Silva dos Santos D, Jorge C, Oliveira-Fusaro M, Parada C
    Neuroscience, 2015;285(0):24-33.
    Species: Rat
    Sample Types: Tissue Homogenates
  35. Zinc prevents sickness behavior induced by lipopolysaccharides after a stress challenge in rats.
    Authors: Kirsten T, Galvao M, Reis-Silva T, Queiroz-Hazarbassanov N, Bernardi M
    PLoS ONE, 2015;10(3):e0120263.
    Species: Rat
    Sample Types: Plasma
  36. S-nitrosoglutathione accelerates recovery from 5-fluorouracil-induced oral mucositis.
    Authors: Skeff M, Brito G, de Oliveira M, Braga C, Cavalcante M, Baldim V, Holanda-Afonso R, Silva-Boghossian C, Colombo A, Ribeiro R, Moura-Neto V, Leitao R
    PLoS ONE, 2014;9(12):e113378.
    Species: Hamster
    Sample Types: Tissue Homogenates
  37. Exploring LPS-induced sepsis in rats and mice as a model to study potential protective effects of the nociceptin/orphanin FQ system
    Authors: Jonathan P Thompson
    Peptides, 2014;61(0):56-60.
    Species: Rat
    Sample Types: Serum
  38. Oligofructose supplementation (10%) during pregnancy and lactation does not change the inflammatory effect of concurrent trans fatty acid ingestion on 21-day-old offspring.
    Authors: Hachul A, Mennitti L, de Oliveira J, Moreno M, Okuda M, Dos Santos B, Oyama L, Ribeiro E, do Nascimento C, Pisani L
    Lipids Health Dis, 2013;12(0):59.
    Species: Rat
    Sample Types: Tissue Homogenates
  39. Effects of freshwater clam extract supplementation on time to exhaustion, muscle damage, pro/anti-inflammatory cytokines, and liver injury in rats after exhaustive exercise.
    Authors: Huang K, Wu W, Yang F, Chiu Y, Peng T, Hsu B, Liao K, Lee R
    Molecules, 2013;18(4):3825-38.
    Species: Rat
    Sample Types: Plasma
  40. Protective effect of hypercapnic acidosis in ischemia-reperfusion lung injury is attributable to upregulation of heme oxygenase-1.
    Authors: Wu S, Li M, Ko F, Wu G, Huang K, Chu S
    PLoS ONE, 2013;8(9):e74742.
    Species: Rat
    Sample Types: BALF
  41. Supernatant of stored platelets causes lung inflammation and coagulopathy in a novel in vivo transfusion model.
    Authors: Vlaar AP, Hofstra JJ, Kulik W
    Blood, 2010;116(8):1360-8.
    Species: Rat
    Sample Types: Tissue Homogenates
  42. Depot-specific modulation of adipokine levels in rat adipose tissue by diet-induced obesity: the effect of aerobic training and energy restriction.
    Authors: Yamashita AS, Lira FS, Rosa JC, Paulino EC, Brum PC, Negrao CE, dos Santos RV, Batista ML, do Nascimento CO, Oyama LM, Seelaender M
    Cytokine, 2010;52(3):168-74.
    Species: Rat
    Sample Types: Tissue Homogenates
  43. TNF-alpha, IL-1beta, IL-6, and cinc-1 levels in rat brain after meningitis induced by Streptococcus pneumoniae.
    Authors: Barichello T, dos Santos I, Savi GD, Simoes LR, Silvestre T, Comim CM, Sachs D, Teixeira MM, Teixeira AL, Quevedo J
    J. Neuroimmunol., 2010;221(1):42-5.
    Species: Rat
    Sample Types: Tissue Homogenates
  44. The lectin-like domain of tumor necrosis factor improves lung function after rat lung transplantation--potential role for a reduction in reactive oxygen species generation.
    Authors: Hamacher J, Stammberger U, Roux J, Kumar S, Yang G, Xiong C, Schmid RA, Fakin RM, Chakraborty T, Hossain HM, Pittet JF, Wendel A, Black SM, Lucas R
    Crit. Care Med., 2010;38(3):871-8.
    Species: Rat
    Sample Types: BALF
  45. Effects of ciliary muscle plasmid electrotransfer of TNF-alpha soluble receptor variants in experimental uveitis.
    Authors: Touchard E, Bloquel C, Bigey P, Kowalczuc L, Jonet L, Thillaye-Goldenberg B, Naud MC, Scherman D, de Kozak Y, Benezra D, Behar-Cohen F
    Gene Ther., 2009;16(7):862-73.
    Species: Rat
    Sample Types: Aqueous Humor
  46. Augmentation of endogenous cannabinoid tone modulates lipopolysaccharide-induced alterations in circulating cytokine levels in rats.
    Authors: Roche M, Kelly JP, O'Driscoll M, O'Driscoll M, Finn DP
    Immunology, 2008;125(2):263-71.
  47. MAPKs are key players in mediating cytokine release and cell death induced by unconjugated bilirubin in cultured rat cortical astrocytes.
    Authors: Fernandes A, Falcao AS, Silva RF, Brito MA, Brites D
    Eur. J. Neurosci., 2007;25(4):1058-68.
    Species: Rat
    Sample Types: Cell Culture Supernates
  48. Sirtuin regulates cigarette smoke-induced proinflammatory mediator release via RelA/p65 NF-kappaB in macrophages in vitro and in rat lungs in vivo: implications for chronic inflammation and aging.
    Authors: Yang SR, Wright J, Bauter M, Seweryniak K, Kode A, Rahman I
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(2):L567-76.
    Species: Rat
    Sample Types: BALF
  49. Inflammatory signalling pathways involved in astroglial activation by unconjugated bilirubin.
    Authors: Fernandes A, Falcao AS, Silva RF, Gordo AC, Gama MJ, Brito MA, Brites D
    J. Neurochem., 2006;96(6):1667-79.
    Species: Rat
    Sample Types: Cell Culture Supernates
  50. Role of matrix metalloproteinases in the inflammatory response in human airway cell-based assays and in rodent models of airway disease.
    Authors: Birrell MA, Wong S, Dekkak A, De Alba J, Haj-Yahia S, Belvisi MG
    J. Pharmacol. Exp. Ther., 2006;318(2):741-50.
    Species: Rat
    Sample Types: BALF
  51. Utility of exhaled nitric oxide as a noninvasive biomarker of lung inflammation in a disease model.
    Authors: Birrell MA, McCluskie K, Hardaker E, Knowles R, Belvisi MG
    Eur. Respir. J., 2006;28(6):1236-44.
    Species: Rat
    Sample Types: BALF
  52. Cardiomyocyte function after burn injury and lipopolysaccharide exposure: single-cell contraction analysis and cytokine secretion profile.
    Authors: Niederbichler AD, Westfall MV, Su GL, Donnerberg J, Usman A, Vogt PM, Ipaktchi KR, Arbabi S, Wang SC, Hemmila MR
    Shock, 2006;25(2):176-83.
    Species: Rat
    Sample Types: Cell Culture Supernates
  53. GM-CSF action in the CNS decreases food intake and body weight.
    Authors: Reed JA, Clegg DJ, Smith KB, Tolod-Richer EG, Matter EK, Picard LS, Seeley RJ
    J. Clin. Invest., 2005;115(11):3035-44.
    Species: Rat
    Sample Types: Serum
  54. CCL20/macrophage inflammatory protein 3alpha and tumor necrosis factor alpha production by primary uterine epithelial cells in response to treatment with lipopolysaccharide or Pam3Cys.
    Authors: Crane-Godreau MA, Wira CR
    Infect. Immun., 2005;73(1):476-84.
    Species: Rat
    Sample Types: Cell Culture Supernates
  55. Effects of estradiol on lipopolysaccharide and Pam3Cys stimulation of CCL20/macrophage inflammatory protein 3 alpha and tumor necrosis factor alpha production by uterine epithelial cells in culture.
    Authors: Crane-Godreau MA, Wira CR
    Infect. Immun., 2005;73(7):4231-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. Maternal poly I:C exposure during pregnancy regulates TNF alpha, BDNF, and NGF expression in neonatal brain and the maternal-fetal unit of the rat.
    Authors: Gilmore JH, Jarskog LF, Vadlamudi S
    J. Neuroimmunol., 2004;159(1):106-12.
    Species: Rat
    Sample Types: Tissue Homogenates
  57. A study of the biologic activity of trauma-hemorrhagic shock mesenteric lymph over time and the relative role of cytokines.
    Authors: Davidson MT, Deitch EA, Lu Q, Osband A, Feketeova E, Nemeth ZH, Hasko G, Xu DZ
    Surgery, 2004;136(1):32-41.
    Species: Rat
    Sample Types: Lymph

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Rat TNF-alpha DuoSet ELISA
By Anonymous on 12/28/2017
Sample Tested: Plasma

Rat TNF-alpha DuoSet ELISA
By Anonymous on 10/06/2016
Sample Tested: Cell culture supernatant