Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit

R&D Systems | Catalog # RRP300

R&D Systems
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Key Product Details

Assay Length

2.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Tissue Homogenates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)

Sensitivity

0.023 ng/mL

Assay Range

0.156-10 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma, Urine)
View all Adiponectin/Acrp30 ELISA Kits »

Product Summary for Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit

The Quantikine Rat Total Adiponectin Immunoassay is a 2.5 hour solid phase ELISA designed to measure rat Adiponectin levels in cell culture supernates, tissue lysates, serum, plasma, and urine. It contains NS0-expressed recombinant rat Adiponectin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat Adiponectin accurately. Results obtained using natural rat Adiponectin showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards.These results indicate that this kit can be used to determine relative mass values for natural rat Adiponectin.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Rat

Specificity

Natural and recombinant rat Adiponectin. This assay recognizes low, medium, and high molecular weight recombinant rat Adiponectin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.587 1.14 3.42 0.595 1.19 3.48
Standard Deviation 0.021 0.025 0.112 0.056 0.083 0.272
CV% 3.6 2.2 3.3 9.4 7.0 7.8

Recovery for Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit

The recovery of rat Adiponectin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=6) 109 100-116
Urine (n=4) 106 91-113

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of rat Adiponectin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Rat Total Adiponectin/Acrp30 ELISA Linearity

Scientific Data Images for Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit

Rat Total Adiponectin/Acrp30 ELISA Standard Curve

Rat Total Adiponectin/Acrp30 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Adiponectin/Acrp30

Adiponectin (Acrp30) is secreted primarily by adipocytes and induces multiple paracrine and endocrine effects on metabolism and inflammation. Adiponectin promotes insulin sensitivity, decreased serum glucose and triglyceride levels, and increased serum glucagon levels. It promotes fatty acid uptake and oxidation, glucose uptake, and lactate production in skeletal muscle. Adiponectin promotes the development of M2 macrophages and promotes the C1qRp mediated clearance of apoptotic cell debris. Decreased plasma HMW Adiponectin levels are associated with upper body obesity, insulin resistance, reduced fatty acid oxidation, dyslipidemia, coronary artery disease, and atherogenesis. Adiponectin forms homotrimers, hexamers, and high molecular weight (HMW) complexes and signals through the receptors AdipoR1 and AdipoR2.

Alternate Names

Acrp30, AdipoQ, ApM1, GBP28

Entrez Gene IDs

9370 (Human); 11450 (Mouse); 246253 (Rat)

Gene Symbol

ADIPOQ

Additional Adiponectin/Acrp30 Products

Product Documents for Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit

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  • Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit
    Name: saleh almarshad
    Sample Tested: Thoracic and Mesenteric SD rats
    Verified Customer | Posted 03/19/2025
    Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit RRP300

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Protocols

View specific protocols for Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit (RRP300):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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