Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit

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RRP300
Rat Total Adiponectin/Acrp30 ELISA Standard Curve
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Citations (11)
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Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
2.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Homogenates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)
Sensitivity
0.023 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant rat Adiponectin. This assay recognizes low, medium, and high molecular weight recombinant rat Adiponectin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Rat Total Adiponectin Immunoassay is a 2.5 hour solid phase ELISA designed to measure rat Adiponectin levels in cell culture supernates, tissue lysates, serum, plasma, and urine. It contains NS0-expressed recombinant rat Adiponectin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat Adiponectin accurately. Results obtained using natural rat Adiponectin showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards.These results indicate that this kit can be used to determine relative mass values for natural rat Adiponectin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 0.587 1.14 3.42 0.595 1.19 3.48
Standard Deviation 0.021 0.025 0.112 0.056 0.083 0.272
CV% 3.6 2.2 3.3 9.4 7 7.8

Recovery

The recovery of rat Adiponectin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=6) 109 100-116
Urine (n=4) 106 91-113

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of rat Adiponectin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Rat Total Adiponectin/Acrp30 ELISA Linearity

Data Examples

Rat Total Adiponectin/Acrp30 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Adiponectin/Acrp30

Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.

Entrez Gene IDs:
9370 (Human); 11450 (Mouse); 246253 (Rat)
Alternate Names:
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. The Preventive Effects of Greenshell Mussel (Perna canaliculus) on Early-Stage Metabolic Osteoarthritis in Rats with Diet-Induced Obesity
    Authors: P Siriarchav, MC Kruger, MR Miller, HS Tian, FM Wolber
    Nutrients, 2019;11(7):.
    Species: Rat
    Sample Types: Plasma
  2. High Protein Diet Induces Oxidative Stress in Rat Cerebral Cortex and Hypothalamus
    Authors: E ?ebrowska, M Maciejczyk, M ?endzian-P, A Zalewska, A Chabowski
    Int J Mol Sci, 2019;20(7):.
    Species: Rat
    Sample Types: Plasma
  3. Effects of Low-Dose Developmental Bisphenol A Exposure on Metabolic Parameters and Gene Expression in Male and Female Fischer 344 Rat Offspring
    Authors: MH Lejonklou, L Dunder, E Bladin, V Pettersson, M Rönn, L Lind, TB Waldén, PM Lind
    Environ. Health Perspect., 2017;125(6):067018.
    Species: Rat
    Sample Types: Plasma
  4. Betaine attenuates chronic alcohol?induced fatty liver by broadly regulating hepatic lipid metabolism
    Authors: W Yang, L Huang, J Gao, S Wen, Y Tai, M Chen, Z Huang, R Liu, C Tang, J Li
    Mol Med Rep, 2017;16(4):5225-5234.
    Species: Rat
    Sample Types: Tissue Homogenates
  5. Adiponectin Improves Coronary No Reflow Injury by Protecting Endothelium in Rats with Type 2 Diabetes Mellitus
    Authors: X Han, Y Wu, X Liu, L Ma, T Lv, Q Sun, W Xu, S Zhang, K Wang, W Wang, X Ma, H Liu
    Biosci. Rep., 2017;0(0):.
    Species: Rat
    Sample Types: Serum
  6. Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gain and Improves Obesity-Related Parameters in High Fat Diet Induced Obese Rats
    Authors: S Samat, F Kanyan Enc, F Nor Hussei, WI Wan Ismail
    Evid Based Complement Alternat Med, 2017;2017(0):1342150.
    Species: Rat
    Sample Types: Serum
  7. Intramyocardial Adipose-Derived Stem Cell Transplantation Increases Pericardial Fat with Recovery of Myocardial Function after Acute Myocardial Infarction
    Authors: Jong-Ho Kim
    PLoS ONE, 2016;11(6):e0158067.
    Species: Rat
    Sample Types: Plasma
  8. Xanthine oxidase inhibition alleviates the cardiac complications of insulin resistance: effect on low grade inflammation and the angiotensin system.
    Authors: El-Bassossy H, Watson M
    J Transl Med, 2015;13(0):82.
    Species: Rat
    Sample Types: Serum
  9. Ameliorative effect of allopurinol on vascular complications of insulin resistance.
    Authors: El-Bassossy H, Elberry A, Azhar A, Ghareib S, Alahdal A
    J Diabetes Res, 2015;2015(0):178540.
    Species: Rat
    Sample Types: Serum
  10. Pentoxifylline alleviates cardiac ischemia and dysfunction following experimental angina in insulin resistance.
    Authors: Azhar A, El-Bassossy H
    PLoS ONE, 2014;9(5):e98281.
    Species: Rat
    Sample Types: Serum
  11. Adverse signaling of scavenger receptor class B1 and PGC1s in alcoholic hepatosteatosis and steatohepatitis and protection by betaine in rat.
    Authors: Varatharajalu R, Garige M, Leckey L, Arellanes-Robledo J, Reyes-Gordillo K, Shah R, Lakshman M
    Am J Pathol, 2014;184(7):2035-44.
    Species: Rat
    Sample Types: Plasma

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