<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED50 for this effect is typically 50-200 ng/mL in the presence of a cross‑linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Chinese Hamster Ovary cell line, CHO-derived Gln27-Pro346, with a C-terminal 10-His tag Accession # O43827
Both Biotinylated rhANGPTL7 (Catalog # BT914) and unlabeled rhANGPTL7 (Catalog # 914-AN) stimulate the expansion of E16-E18 rat liver mononuclear cells in vitro, in the presence of rmSCF (Catalog # 455-MC), rmTpo (Catalog # 488-TO), and rmFlt-3L (Catalog # 427-FL). The ED50 for this effect is typically 50-200 ng/mL in the presence of a crosslinking antibody, His Tag MAb (Catalog # MAB050). The similarity in activity highlights that the biotinylated protein is fully functional.
Background: Angiopoietin-like Protein 7/ANGPTL7
Angiopoietin-like 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted glycoprotein that is structurally related to the angiopoietins. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (1-3). ANGPTL7 shares 89% aa sequence identity with mouse and rat ANGPTL7. It is secreted as a 45-50 kDa monomer that forms disulfide-linked homotrimers and tetramers via the coiled coil domain (4, 5). ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor (3, 5). Its production is up-regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha (5-7). Overexpression of ANGPTL7 in trabecular meshwork cells inhibits the production of collagen and proteoglycans (8). When overexpressed in tumor cells it promotes collagen and proteoglycan deposition but inhibits tumor xenograft progression and tumor angiogenesis (4). ANGPTL7, when used in combination with SCF, Thrombopoietin, IGF-II, and FGF acidic, enhances the expansion and engraftment of human and mouse hematopoietic stem cells (9).
Oike, Y. et al. (2009) Circ. J. 73:2192.
Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70.
Peek, R. et al. (1998) Invest. Opthalmol. Visual Sci. 39:1782.
Peek, R. et al. (2002) J. Biol. Chem. 277:686.
Kuchtey, J. et al. (2008) Invest. Opthalmol. Visual Sci. 49:3438.
Nehme, A. et al. (2009) BMC Med. Genomics 2:58.
Stevens, A.L. et al. (2008) Arthritis Rheum. 58:489.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.