Recombinant Human CD25/IL-2R alpha His Avi-tag Protein, CF

Name: Recombinant Human CD25/IL-2R alpha His Avi-tag Protein, CF
Brand: R&D Systems
SKU: AVI10305
Price: 567 USD
Availability: InStock

R&D Systems | Catalog # AVI10305

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

R&D Systems HEK293-derived Recombinant Human CD25/IL-2R alpha His Avi-tag Protein (AVI10305)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P01589.1

Conjugate

Biotin

Applications

Binding Activity, Bioactivity

View all CD25/IL-2R alpha Proteins and Enzymes »

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Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD25/IL-2R alpha protein

Human CD25/IL-2R alpha (Glu22-Cys213) Accession # P01589.1	Avi-tag	6-His tag
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu22

Predicted Molecular Mass

25 kDa

SDS-PAGE

36-51 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IL-2 (Catalog # 202-IL) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human CD25/IL-2R alpha His-tag Avi-tag (Catalog # AVI10305) binds with an ED₅₀ of 45.0-540 ng/mL.

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Scientific Data Images for Recombinant Human CD25/IL-2R alpha His Avi-tag Protein, CF

Biotinylated Recombinant Human CD25/IL-2R alpha His-tag Avi-tag Protein Binding Activity

When Recombinant Human IL-2 (202-IL) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human CD25/IL-2R alpha His-tag Avi-tag (Catalog # AVI10305) binds with an ED₅₀ of 45.0-540 ng/mL.

Biotinylated Recombinant Human CD25/IL-2R alpha His-tag Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human CD25/IL-2R alpha His-tag Avi-tag (Catalog # AVI10305) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 36-51 kDa.

Formulation, Preparation, and Storage

AVI10305

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD25/IL-2R alpha

IL-2 receptor alpha (IL-2R alpha), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit (gamma c). Human IL-2R alpha cDNA encodes a 213 amino acid (aa) precursor with a 21 aa signal peptide and a 192 aa extracellular region. The ECD of Human IL-2R alpha shares a 59% amino acid sequence identity with the ECD of mouse and rat IL-2R alpha, respectively. IL‑2R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1-3). IL-2R beta (CD122) and gamma c (IL-2R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (4, 5). By itself, IL-2R alpha binds IL-2 with low affinity. IL-2R alpha makes no contacts with IL-2R beta or gamma c, and only minor changes are observed in the IL-2 structure in response to receptor binding. These findings support the principal role of IL-2R alpha to deliver IL-2 to the signaling complex and act as regulator of signal transduction (6, 7). A soluble form of IL‑2R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (8, 9). Increased serum levels of soluble IL‑2R alpha are found in some cancers and immune disorders (10). IL-2R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2R alpha is also required for the development of CD4+CD25+ Treg which suppresses autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (11-13). Our Avi‑tag Biotinylated IL-2Ra features biotinylation at a single site contained within the Avi‑tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity