Recombinant Human CD96 Fc Chimera Protein, CF

R&D Systems | Catalog # 9360-CD

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human CD96 Fc Chimera Protein (9360-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P40200-1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all CD96 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD96 protein
Human CD96
(Lys25-Met519)
Accession # P40200-1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys25

Predicted Molecular Mass

81 kDa

SDS-PAGE

131 - 160 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human CD96 Fc Chimera that produces 50% of the optimal binding response is 0.3-1.8 μg/mL.

Scientific Data Images for Recombinant Human CD96 Fc Chimera Protein, CF

Recombinant Human CD96 Fc Chimera Protein Bioactivity

Recombinant Human CD96 Fc Chimera Protein Bioactivity

When Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human CD96 Fc Chimera (Catalog # 9360-CD) binds with an ED50 of 0.3-1.8 µg/mL.

Formulation, Preparation, and Storage

9360-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD96

Human CD96, also known as Tactile (T cell-activated increased late expression), is a 160 kDa type I transmembrane glycoprotein of the Ig superfamily that shows peak expression 6-9 days after activation of T, NK, and a subpopulation of B cells (1, 2). CD96 is also frequently expressed on acute myeloid leukemia and myelodysplastic stem cells (3, 4). It is expressed on CD4+ and CD8+ T cells, plus NK cells and select B cells (5). Low expression of adhesive human CD96 is a rare cause of C syndrome, a set of developmental anomalies in cranial bone (trigonocephaly), skin and viscera, demonstrating a role for CD96 in development of these tissues (2, 6). Mature human CD96 is a 564 amino acid (aa), type I transmembrane glycoprotein. It contains a 498 aa extracellular region that includes three Ig-like domains. The two N-terminal domains are V-type, while the distal domain is a C-type structure.  Within the ECD, human CD96 shares 53% and 52% aa sequence identity with mouse CD96 and rat CD96, respectively (7). The ECD is highly N- and O-glycosylated (1). Humans express a splice variant with a 16 aa deletion in the second V-type domain, called CD96v2 (2). An 80 kDa soluble form is elevated in human serum during chronic hepatitis B (9). The most N-terminal Ig-like domain of human CD96 binds to CD155/PVR (poliovirus receptor), but CD96/CD155 interaction is species-specific (2, 7, 10). Mouse CD96 also binds nectin-1 (7). On NK cells, co-stimulatory CD96 and DNAM-1 (CD226) are thought to have paired activity with inhibitory TIGIT, all of which bind CD155 and various nectins (11, 12). CD96 can promote NK cell adhesion to, and killing of target cells, including tumors that express CD155 (10, 11).

References

  1. Wang, P.L. et al. (1992) J. Immunol. 148:2600.
  2. Meyer, D. et al. (2009) J. Biol. Chem. 284:2235.
  3. Hosen, N. et al. (2007) Proc. Natl. Acad. Sci. USA 104:11008.
  4. Xie, W. et al. (2010) Cytometry A 77:840.
  5. Eriksson, E. M. et al. (2012) PLOS One 7:e51696.
  6. Kaname, T. et al. (2007) Am. J. Hum. Genet. 81:835.
  7. Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
  8. Protein Accession # BAE32358.
  9. Gong, J. et al. (2008) Clin. Exp. Immunol. 155:207.
  10. Fuchs, A. et al. (2004) J. Immunol. 172:3394.
  11. Stanietsky, N. and O. Mandelboim (2010) FEBS Lett. 584:4895.
  12. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.

Alternate Names

CD96, TACTILE

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Gene Symbol

CD96

UniProt

P40200-1

Additional CD96 Products

Product Documents for Recombinant Human CD96 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD96 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human CD96 Fc Chimera Protein, CF

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