Recombinant Human CEACAM-1/CD66a Fc Chimera Avi Protein, CF

Biotinylated Avi-tag
Catalog # Availability Size / Price Qty
AVI11074-050

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Biotinylated Recombinant Human CEACAM‑1/CD66a Fc Chimera Avi-tag Protein Binding Activity
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Recombinant Human CEACAM-1/CD66a Fc Chimera Avi Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.50 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CEACAM-1/CD66a (Catalog # 2244-CM) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human CEACAM-1/CD66a Fc Chimera Avi-tag (Catalog # AVI11074) binds with an ED50 of 5.00-50.0 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human CEACAM-1/CD66a protein
Human CEACAM-1
(Gln35-Gly428)
Accession # P13688.2
IEGRMDHuman IgG1
(Pro100-Lys330)
Avi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Protein identity confirmed by mass spectrometry
Structure / Form
Disulfide-linked homodimer
Biotinylated via Avi-tag
Predicted Molecular Mass
72 kDa
SDS-PAGE
115-135 kDa, under reducing conditions.

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AVI11074

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11074

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human CEACAM-1/CD66a (2244-CM) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human CEACAM-1/CD66a Fc Chimera Avi-tag Protein (Catalog # AVI11074) binds with an ED50 of 5.00-50.0 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human CEACAM‑1/CD66a Fc Chimera Avi-tag Protein (Catalog # AVI11074) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 115-135 kDa and 230-270 kDa, respectively.

Reconstitution Calculator

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Background: CEACAM-1/CD66a

Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1), also known as cluster of differentiation 66a (CD66a) and biliary glycoprotein (BGP), is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. The CEACAM family is involved in diverse cellular functions from cell adhesion and differentiation to proliferation, and survival and are utilized by several bacterial pathogens to bind and invade host cells (1-3). Mature human CEACAM-1 consists of an extracellular domain (ECD) with 1 V-type Ig-like domain and 3 C2-type Ig-like domains, a transmembrane domain, and a cytoplasmic region shows one ITIM motif and a calmodulin binding site (4). CEACAM-1 is unique among the CEACAM family as it is the only family member to contain the inhibitory ITIM domains. The ECD of human CEACAM-1 shares 54% and 52% amino acid sequence identity with mouse and rat CEACAM-1, respectively. Several alternative splice variants of CEACAM-1 have been reported with alterations occurring in both the ECD and cytoplasmic region, though the function of the isoforms remain poorly understood (5). CEACAM-1 can be expressed in human epithelial, endothelial, and hematopoietic cells and is involved in morphogenesis, apoptosis, angiogenesis, and cell proliferation as well as playing a role in innate and adaptive immunity (6,7). CEACAM-1 functions through either homophilic interactions with CEACAM-1 variants or binds in a heterophilic manner to other CEACAMs, including CEACAM-5, CEACAM-6, and CEACAM-8 (7). Several pathogens from Escherichia coli and Neisseria gonorrhoeae to N. meningitidis use CEACAM-1 as a ligand as a step in bacterial invasion of the host (8). CEACAM-1 signaling is associated with inhibition of proliferation and misregulation of its expression is often reported in cancer (5,9,10). As such, CEACAM-1 is being studied as a clinical biomarker and/or promising therapeutic target for several cancer types including: non-small cell lung cancer, pancreatic, colorectal, bladder, and melanomas (5,9,10). Our Avi-tag Biotinylated CEACAM-1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. Tchoupa, A. et al. (2014) J Cell Commun Signal 12:27.
  2. Hauck, C.R. et al. (2006) Eur J Cell Biol. 85:235.
  3. Kuespert, K. et al. (2006) Curr. Opin. Cell Biol 18:565.
  4. Kim, W.M. et al. (2019) Seminars in immunology 42:101296.
  5. Dankner, M. et al. (2017) Oncoimmunology. 6:e1328336.
  6. Gandhi, A.K. et al. (2021) Commun Biol 4:360.
  7. Helfrich, I. and Singer, B.B. (2019) Cancers, 11:356.
  8. Voges, M. et al. (2010) BMC Microbiol. 10:117.
  9. Beauchemin, N. and Arabzadeh, A. (2013) Cancer Metastasis Rev. 32:643.
  10. Fiori, V. et al. (2012) Ann Ist Super Sanita. 48:161.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 1
Entrez Gene IDs
634 (Human); 26365 (Mouse); 102139857 (Cynomolgus Monkey)
Alternate Names
antigen CD66; BGP1; BGP-1; BGP1BGPBiliary glycoprotein 1; BGPI; Biliary Glycoprotein 1; biliary glycoprotein adhesion molecule; carcinoembryonic antigen-related cell adhesion molecule 1 (biliaryglycoprotein); carcinoembryonic antigen-related cell adhesion molecule 1; CD66a antigen; CD66a; Cea-1; CEACAM1; CEACAM-1; Hv-1; Hv-2; MHVR

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