Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein, CF

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Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein Binding Activity
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Product Details
Citations (1)
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Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Human EGFR Isoform vIII antibody (Novus Catalog # 50599) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human EGFR Isoform vIII Fc Chimera Avi-tag (Catalog # AVI10494)binds with an ED50 of 25-150 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived human EGFR protein
Human EGFR Isoform vIII
Accession # NP_001333870.1
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer, biotinylated via Avi-tag
Predicted Molecular Mass
67 kDa
95-115 kDa, under reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein Binding Activity View Larger

When Human EGFR Isoform vIII antibody (Novus Catalog # BNP-50599) is immobilized at 0.1 μg/mL (100 μL/well), Biotinylated Recombinant Human EGFR Isoform vIII Fc Chimera Avi-tag (AVI10494) binds with an ED50 of 25-150 ng/mL.

SDS-PAGE Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein (AVI10494) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-115 kDa and 190-230 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: EGFR

Epidermal growth factor receptor (EGFR), also known as HER-1 and ErbB1, is a member of a subfamily of receptor tyrosine kinases comprised of four members: EGFR, ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular domain (ECD) containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). Several soluble isoforms lacking the intracellular domain are generated by alternate splicing (3‑4). EGFRvIII is a tumor‐specific mutation that results from an in‐frame deletion removing 267 amino acids from the ECD and insertion of a glycine residue (5). EGFRvIII has a molecular mass of approximately 145 kDa and has been shown to have weaker activity than full-length EGFR (6). EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (7‑9). EGFR can also be recruited to form heterodimers with the ligand‑activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (7-9). EGFR is overexpressed in a wide variety of tumors, with EGFRvIII overexpressed particularly in glioblastoma multiforme (GMB), and is the target of several anti-cancer therapeutics (5,10,11). Our Avi-tag Biotinylated Recombinant Human EGFRvIII features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

  1. Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
  2. Shilo, B.Z. (2005) Development 132:4017.
  3. Guillaudeau, A. et al. (2012) PLoS One. 7:1.
  4. Reiter J.L. et al. (2001) Genomics 71:1.
  5. Gan HK et al. (2013) FEBS J. 280:5350
  6. Batra SK, et al. (1995) Cell Growth Differ 6:1251
  7. Freed, D. M. et al. (2017) Cell. 171:683.
  8. Burgess, A.W. et al. (2003) Mol. Cell 12:541.
  9. Faria, J. A. et al. (2016) BBRC. 478:39.
  10. An Z. et al. (2018) Oncogene. 37:1561.
  11. Lee, C. K. et al. (2017) J. Thoracic Oncology. 12:403.
Long Name
Epidermal Growth Factor Receptor
Entrez Gene IDs
1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Citation for Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A Rational Designed Novel Bispecific Antibody for the Treatment of GBM
    Authors: R Sun, Y Zhou, L Han, Z Pan, J Chen, H Zong, Y Bian, H Jiang, B Zhang, J Zhu
    Biomedicines, 2021;9(6):.
    Species: Human
    Sample Types:
    Applications: Surface Plasmon Resonance (SPR


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