ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85. Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation
Recombinant Human ErbB3/Her3 Fc Chimera Protein, CF
R&D Systems | Catalog # 348-RB
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Key Product Details
- R&D Systems NS0-derived Recombinant Human ErbB3/Her3 Fc Chimera Protein (348-RB)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Disulfide-linked homodimer
Applications
Bioactivity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived human ErbB3/Her3 protein
| Human ErbB3 (Ser20-Thr643) Accession # P21860 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser20
Predicted Molecular Mass
96 kDa (monomer)
SDS-PAGE
130-140 kDa, reducing conditions
Activity
Measured by its ability to inhibit the biological activity of Neuregulin-1-beta 1 on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.
The ED50 for this effect is 1.5-6.0 µg/mL in the presence of 10 ng/mL Recombinant Human NRG1‑ beta 1/HRG1‑ beta 1 Extracellular Domain (Catalog # 377-HB).
The ED50 for this effect is 1.5-6.0 µg/mL in the presence of 10 ng/mL Recombinant Human NRG1‑ beta 1/HRG1‑ beta 1 Extracellular Domain (Catalog # 377-HB).
Reviewed Applications
Read 2 reviews rated 5 using 348-RB in the following applications:
Formulation, Preparation, and Storage
348-RB
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: ErbB3/Her3
References
- Kraus, M.H. et. al. (1989) Proc. Natl. Acad. Sci. 86:9193.
- Plowman, G.D. et. al. (1990) Proc. Natl. Acad. Sci. USA 87:4905.
- Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
- Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
- Sundaresan, S. et. al. (1998) Endocrinology 139:4756.
- Hellyer, N.J. et. al. (1998) Biochem. J. 333:757.
- Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
- Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
- Schlessinger, J. (2000) Cell 103:211.
- Daly, R.J. (1999) Growth Factors 16:255.
Long Name
Receptor Tyrosine Protein Kinase ErbB3
Alternate Names
HER3
Gene Symbol
ERBB3
UniProt
Additional ErbB3/Her3 Products
Product Documents for Recombinant Human ErbB3/Her3 Fc Chimera Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human ErbB3/Her3 Fc Chimera Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human ErbB3/Her3 Fc Chimera Protein, CF
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Customer Reviews for Recombinant Human ErbB3/Her3 Fc Chimera Protein, CF (2)
5 out of 5
2 Customer Ratings
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Application: Immunoassay StandardVerified Customer | Posted 05/07/2019
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Associated Pathways
MAPK Signaling Pathway: Mitogen Stimulation Pathway
mTOR Signaling Pathway
