Recombinant Human Glypican 3 His-tag Avi-tag Protein, CF

R&D Systems | Catalog # AVI2119

Biotinylated
R&D Systems
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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Glypican 3 His-tag Avi-tag Protein (AVI2119)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P51654.1

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity
View all Glypican 3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Glypican 3 protein
Human Glypican 3
(Gln25-His559)
Accession # P51654.1		 HHHHHH  Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Sequence confirmed by mass spectrometry.

Predicted Molecular Mass

63 kDa

SDS-PAGE

90-140 kDa under non-reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human FGF basic/FGF2 (Catalog # 233-FB/CF) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human Glypican 3 His-tag Avi-tag (Catalog # AVI2119) binds with an ED50 of 20-100 ng/mL.

Scientific Data Images for Recombinant Human Glypican 3 His-tag Avi-tag Protein, CF

Recombinant Human Glypican 3 His-tag Avi-tag Protein Binding Activity

Recombinant Human Glypican 3 His-tag Avi-tag Protein Binding Activity

When Recombinant Human FGF basic/FGF2/bFGF (146aa) Protein (233-FB/CF) is immobilized at 0.1 μg/mL (100 μL/well), Biotinylated Recombinant Human Glypican-3 His-tag Avi-tag (AVI2119) binds with an ED50 of 20-120 ng/mL.

Formulation, Preparation, and Storage

AVI2119
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glypican 3

Glypican 3 (GPC3), also known as GTR2-2, intestinal protein OCI-5 and MXR7, is a member of the heparan sulfate (HS) proteoglycan family (1).  In mammals, six glypican family members have been identified, all sharing a conserved core protein that is linked to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor. GPC3 can be cleaved by furin to produce two subunits that are linked by disulfide bonds: a 40 kDa N-terminal alpha subunit that can be secreted into the blood and a 30 kDa membrane-bound C-terminal beta subunit containing two HS glycan chains (1-3). Several isoforms of GPC3 due to alternative splicing have been reported (1). Within the mature extracellular domain, human GPC3 shares 96% amino acid sequence identity with both mouse and rat GPC3.  GPC3 is an important biomarker present in the serum of hepatocellular carcinoma patients, which distinguishes benign from cancerous nodules (4). Glypican 3 is over-expressed in hepatocellular carcinomas and binding of GPC3 to CD81 promotes development of carcinomas by activation of Hippo pathways in hepatocytes (5).  Our Avi-tag Biotinylated Recombinant  Glypican 3  features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide.  Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Ho, M. and Kim, H. (2012) Eur. J. Cancer. 47:333.
  2. Haruyama, Y. and Kataoka, H. (2016) World J. Gastroenterol. 22:275.
  3. Shimizu, Y. et al. (2019) Front Oncol. 9:248.
  4. Ge, S. et al. (2018) Filmus, Int J Clin Exp Pathol. 11(12):5774.
  5. Xue, Y. et al. (2018) Am J Pathol. 188(6):1469.

Alternate Names

GPC3

Entrez Gene IDs

2719 (Human); 14734 (Mouse); 25236 (Rat); 102137748 (Cynomolgus Monkey)

Gene Symbol

GPC3

UniProt

P51654.1

Additional Glypican 3 Products

Product Documents for Recombinant Human Glypican 3 His-tag Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human Glypican 3 His-tag Avi-tag Protein, CF

For research use only

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