Recombinant Human IL-2 (Mammalian-expressed) Protein, CF Summary
The ED50 for this effect is 0.05-0.3 ng/mL.
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.|
|Reconstitution||Reconstitute at 100-500 μg/mL in sterile 100 mM Acetic Acid|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Interleukin-2 (IL-2) is a 15.5 kDa cytokine which is part of the common gamma chain family which includes IL-4, IL-7, IL-9, IL-15 and IL-21 (1). IL-2 contains a four alpha-helix bundle, an O-linked glycosylation site (Thr 23) and one disulfide bond (Cys 78 ↔ Cys 125). It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (2-3). Mature human IL-2 shares 56% and 66% amino acid sequence identity with mouse and rat IL-2, respectively. The receptor for IL-2 consists of three subunits(alpha, beta, gamma) that are present on the cell surface in varying preformed complexes. The 55 kDa IL-2 R alpha (CD25) is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta(CD122), which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma(CD132), which is shared with the receptors for IL-4, -7, -9, -15, and -21, is recruited after IL-2 binds to IL-2R alpha and IL-2R beta (4). Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (5). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (6-8). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (9, 10).
- Lin, J.X. Leonard, W.J. (2018) Cold Spring Harb Perspect Biol. 10:9.
- Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
- Taniguchi, T. et al. (1983) Nature 302:305.
- Liao W, Lin J.X. Leonard W.J. (2011) Curr Opin Immunol. 23:598.
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- Laurence, A. et al. (2007) Immunity 26:371.
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- Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
- Fehervari, Z. et al. (2006) Trends Immunol. 27:109.
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