Recombinant Human IL-2 (Mammalian-expressed) Protein, CF

Catalog # Availability Size / Price Qty
10453-IL-010
10453-IL-050
10453-IL-100
10453-IL-250
10453-IL-500
10453-IL-01M
Recombinant Human IL-2 (Mammalian-expressed) Protein Bioactivity
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Recombinant Human IL-2 (Mammalian-expressed) Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295.

The ED50 for this effect is 0.05-0.3 ng/mL.

Source
Chinese Hamster Ovary cell line, CHO-derived human IL-2 protein
Ala21-Thr153
Accession #
N-terminal Sequence
Analysis
Ala21
Structure / Form
Monomer
Predicted Molecular Mass
15 kDa
SDS-PAGE
14-18 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10453-IL

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100-500 μg/mL in sterile 100 mM Acetic Acid
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Human IL-2 (Mammalian-expressed) Protein Bioactivity View Larger

Recombinant Human IL-2 (Mammalian-expressed) Protein (10453-IL) stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.3 ng/mL. Mammalian-expressed Recombinant Human IL-2 has similar activity to E.coli-derived Recombinant Human IL-2 (202-IL).

SDS-PAGE Recombinant Human IL-2 (Mammalian-expressed) Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human IL-2 (Mammalian-expressed) Protein (10453-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 14-18 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-2

Interleukin-2 (IL-2) is a 15.5 kDa cytokine which is part of the common gamma chain family which includes IL-4, IL-7, IL-9, IL-15 and IL-21 (1). IL-2 contains a four alpha-helix bundle, an O-linked glycosylation site (Thr 23) and one disulfide bond (Cys 78 ↔ Cys 125). It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (2-3). Mature human IL-2 shares 56% and 66% amino acid sequence identity with mouse and rat IL-2, respectively. The receptor for IL-2 consists of three subunits(alpha, beta, gamma) that are present on the cell surface in varying preformed complexes. The 55 kDa IL-2 R alpha (CD25) is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta(CD122), which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma(CD132), which is shared with the receptors for IL-4, -7, -9, -15, and -21, is recruited after IL-2 binds to IL-2R alpha and IL-2R beta (4). Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (5). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (6-8). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (9, 10).

References
  1. Lin, J.X. Leonard, W.J. (2018) Cold Spring Harb Perspect Biol. 10:9.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Taniguchi, T. et al. (1983) Nature 302:305.
  4. Liao W, Lin J.X. Leonard W.J. (2011) Curr Opin Immunol. 23:598.
  5. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  6. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  7. Laurence, A. et al. (2007) Immunity 26:371.
  8. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  9. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  10. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.
Long Name
Interleukin 2
Entrez Gene IDs
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF

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