Recombinant Human Leptin Protein, CF

(13 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human Leptin R. The ED50 for this effect is typically 0.4‑2 ng/mL.
  • Source
    E. coli-derived Val22-Cys167, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
  • Predicted Molecular Mass
    16 kDa
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Reconstitution Reconstitute at 1 mg/mL in sterile 20 mM Tris-HCl, pH 8.0.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Data Images

1 μg/lane of Recombinant Human Leptin/OB was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.

Recombinant Human Leptin/OB (Catalog # 398-LP) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with human Leptin R. The ED50 for this effect is typically 0.4-2 ng/mL.

Background: Leptin/OB

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the sythesis of Leptin have been found to be obese, diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1 - 3). The roles of leptin in body weight regulation, hematopoiesis and reproduction continue to be investigated.

  • References:
    1. Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
    2. Tartaglia, L.A. et al. (1995) Cell 83:1263.
    3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
  • Entrez Gene IDs:
    3952 (Human); 16846 (Mouse); 25608 (Rat)
  • Alternate Names:
    FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 10 of 13
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Sample Type
  1. Functional human antibody CDR fusions as long-acting therapeutic endocrine agonists.
    Authors: Liu, Tao, Zhang, Yong, Liu, Yan, Wang, Ying, Jia, Haiqun, Kang, Mingchao, Luo, Xiaozhou, Caballero, Dawna, Gonzalez, Jose, Sherwood, Lance, Nunez, Vanessa, Wang, Danling, Woods, Ashley, Schultz, Peter G, Wang, Feng
    Proc Natl Acad Sci U S A, 2015;112(5):1356-61.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Leptin promotes wound healing in the skin.
    Authors: Tadokoro, Susumu, Ide, Shinji, Tokuyama, Reiko, Umeki, Hirochik, Tatehara, Seiko, Kataoka, Shiki, Satomura, Kazuhito
    PLoS ONE, 2015;10(3):e0121242.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Low adiposity during early infancy is associated with a low risk for developing dengue hemorrhagic fever: a preliminary model.
    Authors: Libraty D, Zhang L, Woda M, Giaya K, Kathivu C, Acosta L, Tallo V, Segubre-Mercado E, Bautista A, Obcena A, Brion J, Capeding R
    PLoS ONE, 2014;9(2):e88944.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Glycodelin-A protein interacts with Siglec-6 protein to suppress trophoblast invasiveness by down-regulating extracellular signal-regulated kinase (ERK)/c-Jun signaling pathway.
    Authors: Lam KK, Chiu PC, Lee CL
    J. Biol. Chem., 2011;286(43):37118-27.
    Species: Human
    Sample Type: recombinant siglec-6
    Application: Competitive Binding Assay
  5. Leptin enhances survival and induces migration, degranulation, and cytokine synthesis of human basophils.
    Authors: Suzukawa M, Nagase H, Ogahara I, Han K, Tashimo H, Shibui A, Koketsu R, Nakae S, Yamaguchi M, Ohta K
    J. Immunol., 2011;186(9):5254-60.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
  7. Leptin-stimulated endothelial nitric-oxide synthase via an adenosine 5'-monophosphate-activated protein kinase/Akt signaling pathway is attenuated by interaction with C-reactive protein.
    Authors: Procopio C, Andreozzi F, Laratta E, Cassese A, Beguinot F, Arturi F, Hribal ML, Perticone F, Sesti G
    Endocrinology, 2009;150(8):3584-93.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  8. Absence of leptin resistance in platelets from morbidly obese individuals may contribute to the increased thrombosis risk in obesity.
    Authors: Dellas C, Schafer K, Rohm I, Lankeit M, Ellrott T, Faustin V, Riggert J, Hasenfuss G, Konstantinides S
    Thromb. Haemost., 2008;100(6):1123-9.
    Species: Human
    Sample Type: Plasma
    Application: Bioassay
  9. Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
    Authors: Emsley HC, Smith CJ, Gavin CM
    BMC Neurol, 2007;7(0):5.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  10. Steroids and protein markers in the follicular fluid as indicators of oocyte quality in patients with and without endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    J. Assist. Reprod. Genet., 2005;22(6):257-64.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  11. Leptin induces a novel form of NMDA receptor-dependent long-term depression.
    Authors: Durakoglugil M, Irving AJ, Harvey J
    J. Neurochem., 2005;95(2):396-405.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  12. BiaCore analysis of leptin-leptin receptor interaction: evidence for 1:1 stoichiometry.
    Authors: Mistrik P, Moreau F, Allen JM
    Anal. Biochem., 2004;327(2):271-7.
  13. Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes.
    Authors: Kaser S, Kaser A, Vogel W, Patsch JR, Tilg H
    Eur. Cytokine Netw., 2002;13(2):225-9.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
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