Recombinant Human Leptin Protein, CF

Catalog # Availability Size / Price Qty
398-LP-01M
398-LP-05M
Recombinant Human Leptin Protein Bioactivity
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Product Details
Citations (36)
FAQs
Reviews (2)

Recombinant Human Leptin Protein, CF Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human Leptin R. The ED50 for this effect is 0.4‑2 ng/mL.
Source
E. coli-derived human Leptin/OB protein
Val22-Cys167, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
16 kDa

Product Datasheets

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398-LP

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

398-LP

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Reconstitution Reconstitute at 1 mg/mL in sterile 20 mM Tris-HCl, pH 8.0.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Human Leptin Protein Bioactivity View Larger

Recombinant Human Leptin/OB (Catalog # 398-LP) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with human Leptin R. The ED50 for this effect is 0.4-2 ng/mL.

SDS-PAGE Recombinant Human Leptin Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human Leptin/OB was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.

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Background: Leptin/OB

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the sythesis of Leptin have been found to be obese, diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1 - 3). The roles of leptin in body weight regulation, hematopoiesis and reproduction continue to be investigated.

References
  1. Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
  2. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
Entrez Gene IDs
3952 (Human); 16846 (Mouse); 25608 (Rat)
Alternate Names
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS

Citations for Recombinant Human Leptin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Leptin-depended NLRP3 inflammasome activation in osteoarthritic chondrocytes is mediated by ROS
    Authors: E Mourmoura, I Papathanas, V Trachana, V Konteles, A Tsoumpou, A Goutas, AA Papageorgi, N Stefanou, A Tsezou
    Mechanisms of Ageing and Development, 2022;208(0):111730.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Role of leptin/ILC2 axis in allergic rhinitis in obese children
    Authors: X Wang, Y Shen, X Ke, H Kang, S Hong
    International journal of pediatric otorhinolaryngology, 2022;157(0):111127.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Leptin-mediated proinflammatory bone marrow environment in acquired aplastic anemia
    Authors: M Gao, M Ge, J Huo, X Ren, X Li, Y Shao, J Huang, J Zhang, M Wang, N Nie, P Jin, Y Zheng
    Cytokine, 2022;152(0):155829.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Ectopic Leptin Production by Intraocular Pancreatic Islet Organoids Ameliorates the Metabolic Phenotype of ob/ob Mice
    Authors: B Leibiger, T Moede, I Valladolid, M Paschen, M Visa, IB Leibiger, PO Berggren
    Metabolites, 2021;11(6):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. The Role of Leptin in Childhood Immune Thrombocytopenia (ITP): An Anti-Inflammatory Agent?
    Authors: I Thomas, I Panagoulia, I Aggeletopo, A Varvarigou, BE Spiliotis, A Mouzaki
    International Journal of Molecular Sciences, 2021;22(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Appetite Control across the Lifecourse: The Acute Impact of Breakfast Drink Quantity and Protein Content. The Full4Health Project
    Authors: DR Crabtree, W Buosi, CL Fyfe, GW Horgan, Y Manios, O Androutsos, A Giannopoul, G Finlayson, K Beaulieu, CL Meek, JJ Holst, KV Van Norren, JG Mercer, AM Johnstone
    Nutrients, 2020;12(12):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA (Standard)
  7. Chronic Gq signaling in AgRP neurons does not cause obesity
    Authors: SN Ewbank, CA Campos, JY Chen, AJ Bowen, SL Padilla, JL Dempsey, JY Cui, RD Palmiter
    Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Leptin contributes to the taxol chemoresistance in epithelial ovarian cancer
    Authors: F Gu, H Zhang, L Yao, S Jiang, H Lu, X Xing, C Zhang, P Jiang, R Zhang
    Oncol Lett, 2019;18(1):561-570.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Leptin contributes to the taxol chemoresistance in epithelial ovarian cancer
    Authors: F Gu, H Zhang, L Yao, S Jiang, H Lu, X Xing, C Zhang, P Jiang, R Zhang
    Oncol Lett, 2019;18(1):561-570.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Leptin enhances cytokine/chemokine production by normal lung fibroblasts by binding to leptin receptor
    Authors: K Watanabe, M Suzukawa, S Arakawa, K Kobayashi, S Igarashi, H Tashimo, H Nagai, S Tohma, T Nagase, K Ohta
    Allergol Int, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Leptin increases mitochondrial OPA1 via GSK3-mediated OMA1 ubiquitination to enhance therapeutic effects of mesenchymal stem cell transplantation
    Authors: F Yang, R Wu, Z Jiang, J Chen, J Nan, S Su, N Zhang, C Wang, J Zhao, C Ni, Y Wang, W Hu, Z Zeng, K Zhu, X Liu, X Hu, W Zhu, H Yu, J Huang, J Wang
    Cell Death Dis, 2018;9(5):556.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Leptin affects filopodia and cofilin in NK-92 cells in a dose- and time-dependent manner
    Authors: J Oswald, M Büttner, S Jasinsky-B, R Jacobs, P Rosenstock, H Kielstein
    Eur J Histochem, 2018;62(1):2848.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. The adipokine leptin modulates adventitial pericyte functions by autocrine and paracrine signalling
    Authors: F Riu, SC Slater, EJ Garcia, I Rodriguez-, V Alvino, E Avolio, G Mangialard, A Cordaro, S Satchell, C Zebele, A Caporali, G Angelini, P Madeddu
    Sci Rep, 2017;7(1):5443.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Type II Endometrial Cancer Overexpresses NILCO: A Preliminary Evaluation
    Authors: D Daley-Brow, G Oprea-Iles, KT Vann, V Lanier, R Lee, PV Candelaria, A Quarshie, R Pattillo, RR Gonzalez-P
    Dis. Markers, 2017;2017(0):8248175.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Leptin-Notch signaling axis is involved in pancreatic cancer progression
    Authors: A Harbuzariu, A Rampoldi, DS Daley-Brow, P Candelaria, TL Harmon, CC Lipsey, DJ Beech, A Quarshie, GO Ilies, RR Gonzalez-P
    Oncotarget, 2017;8(5):7740-7752.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Leptin pharmacokinetics in male mice
    Authors: Robert A Hart
    Endocr Connect, 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  17. Leptin promotes wound healing in the skin.
    Authors: Tadokoro, Susumu, Ide, Shinji, Tokuyama, Reiko, Umeki, Hirochik, Tatehara, Seiko, Kataoka, Shiki, Satomura, Kazuhito
    PLoS ONE, 2015;10(3):e0121242.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Functional human antibody CDR fusions as long-acting therapeutic endocrine agonists.
    Authors: Liu, Tao, Zhang, Yong, Liu, Yan, Wang, Ying, Jia, Haiqun, Kang, Mingchao, Luo, Xiaozhou, Caballero, Dawna, Gonzalez, Jose, Sherwood, Lance, Nunez, Vanessa, Wang, Danling, Woods, Ashley, Schultz, Peter G, Wang, Feng
    Proc Natl Acad Sci U S A, 2015;112(5):1356-61.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Leptin-STAT3-G9a Signaling Promotes Obesity-Mediated Breast Cancer Progression.
    Authors: Chang C, Wu M, Yang J, Camarillo I, Chang C
    Cancer Res, 2015;75(11):2375-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Leptin-mediated regulation of ICAM-1 is Rho/ROCK dependent and enhances gastric cancer cell migration.
    Authors: Dong, Z, Fu, S, Xu, X, Yang, Y, Du, L, Li, W, Kan, S, Li, Z, Zhang, X, Wang, L, Li, J, Liu, H, Qu, X, Wang, C
    Br J Cancer, 2014;110(7):1801-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Low adiposity during early infancy is associated with a low risk for developing dengue hemorrhagic fever: a preliminary model.
    Authors: Libraty D, Zhang L, Woda M, Giaya K, Kathivu C, Acosta L, Tallo V, Segubre-Mercado E, Bautista A, Obcena A, Brion J, Capeding R
    PLoS ONE, 2014;9(2):e88944.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. TNF-alpha induces CXCL1 chemokine expression and release in human vascular endothelial cells in vitro via two distinct signaling pathways.
    Authors: Lo H, Lai T, Li C, Wu W
    Acta Pharmacol Sin, 2014;35(3):339-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Discovery of a novel functional leptin protein (LEP) in zebra finches: evidence for the existence of an authentic avian leptin gene predominantly expressed in the brain and pituitary.
    Authors: Huang, Guian, Li, Juan, Wang, Hongning, Lan, Xinyu, Wang, Yajun
    Endocrinology, 2014;155(9):3385-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Leptin up-regulates TLR2 in human monocytes.
    Authors: Jaedicke K, Roythorne A, Padget K, Todryk S, Preshaw P, Taylor J
    J Leukoc Biol, 2013;93(4):561-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Leptin enhances the secretion of interleukin (IL)-18, but not IL-1beta, from human monocytes via activation of caspase-1.
    Authors: Jitprasertwong P, Jaedicke K, Nile C, Preshaw P, Taylor J
    Cytokine, 2013;65(2):222-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Leptin-dependent and leptin-independent paracrine effects of perivascular adipose tissue on neointima formation.
    Authors: Schroeter M, Eschholz N, Herzberg S, Jerchel I, Leifheit-Nestler M, Czepluch F, Chalikias G, Konstantinides S, Schafer K
    Arterioscler Thromb Vasc Biol, 2013;33(5):980-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Glycodelin-A protein interacts with Siglec-6 protein to suppress trophoblast invasiveness by down-regulating extracellular signal-regulated kinase (ERK)/c-Jun signaling pathway.
    Authors: Lam KK, Chiu PC, Lee CL
    J. Biol. Chem., 2011;286(43):37118-27.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  28. Leptin enhances survival and induces migration, degranulation, and cytokine synthesis of human basophils.
    Authors: Suzukawa M, Nagase H, Ogahara I, Han K, Tashimo H, Shibui A, Koketsu R, Nakae S, Yamaguchi M, Ohta K
    J. Immunol., 2011;186(9):5254-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
  30. Leptin-stimulated endothelial nitric-oxide synthase via an adenosine 5'-monophosphate-activated protein kinase/Akt signaling pathway is attenuated by interaction with C-reactive protein.
    Authors: Procopio C, Andreozzi F, Laratta E, Cassese A, Beguinot F, Arturi F, Hribal ML, Perticone F, Sesti G
    Endocrinology, 2009;150(8):3584-93.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  31. Absence of leptin resistance in platelets from morbidly obese individuals may contribute to the increased thrombosis risk in obesity.
    Authors: Dellas C, Schafer K, Rohm I, Lankeit M, Ellrott T, Faustin V, Riggert J, Hasenfuss G, Konstantinides S
    Thromb. Haemost., 2008;100(6):1123-9.
    Species: Human
    Sample Types: Plasma
    Applications: Bioassay
  32. Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
    Authors: Emsley HC, Smith CJ, Gavin CM
    BMC Neurol, 2007;7(0):5.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  33. Leptin induces a novel form of NMDA receptor-dependent long-term depression.
    Authors: Durakoglugil M, Irving AJ, Harvey J
    J. Neurochem., 2005;95(2):396-405.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  34. Steroids and protein markers in the follicular fluid as indicators of oocyte quality in patients with and without endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    J. Assist. Reprod. Genet., 2005;22(6):257-64.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  35. BiaCore analysis of leptin-leptin receptor interaction: evidence for 1:1 stoichiometry.
    Authors: Mistrik P, Moreau F, Allen JM
    Anal. Biochem., 2004;327(2):271-7.
  36. Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes.
    Authors: Kaser S, Kaser A, Vogel W, Patsch JR, Tilg H
    Eur. Cytokine Netw., 2002;13(2):225-9.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)

FAQs

  1. Can I reconstitute this in PBS?

    • We strongly recommend reconstituting this protein in 20 mM Tris-HCI, pH 8.0 as indicated on the product insert. This is the most stable buffer we have found for reconstitution and subsequent storage of this protein.

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Reviews for Recombinant Human Leptin Protein, CF

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Recombinant Human Leptin Protein, CF
By Balaji Mahender on 12/18/2017
Application: Immunoassay Standard

Recombinant Human Leptin Protein, CF
By Kurt Shanebeck on 12/18/2017
Application: In vitro bioactivity in cell culture