Recombinant Human M-CSF (CHO-expressed) Protein

Carrier Free

Catalog # Availability Size / Price Qty
216-MCC-010/CF
216-MCC-025/CF
216-MCC-100/CF

With Carrier

Catalog # Availability Size / Price Qty
216-MCC-010
Recombinant Human M-CSF (CHO-expressed) Protein Bioactivity
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Product Details
Citations (2)
FAQs
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Recombinant Human M-CSF (CHO-expressed) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860. The ED50 for this effect is 0.6-2.4 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human M-CSF protein
Glu33-Arg255
Accession #
N-terminal Sequence
Analysis
Glu33
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
25.1 kDa (monomer)
SDS-PAGE
41-50 kDa, reducing conditions

Product Datasheets

216-MCC (with carrier)

216-MCC/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

216-MCC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

216-MCC/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human M-CSF (CHO-expressed) Protein Bioactivity View Larger

Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.6-2.4 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: M-CSF

M-CSF, also known as CSF-1, is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O-glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode
M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of canine, bovine, mouse and rat M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species specific.

References
  1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
  2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
  4. Ryan, G.R. et al. (2001) Blood 98:74.
  5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
  6. Nandi, S. et al. (2006) Blood 107:786.
  7. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
  8. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
  9. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
  10. Koths, K. (1997) Mol. Reprod. Dev. 46:31.
  11. Jang, M-H. et al. (2006) J. Immunol. 177:4055.
  12. Kawasaki, E.S. et al. (1985) Science 230: 291.
  13. Wong, G.G. et al. (1987) Science 235:1504.
Long Name
Macrophage Colony Stimulating Factor
Entrez Gene IDs
1435 (Human); 12977 (Mouse)
Alternate Names
colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930

Citations for Recombinant Human M-CSF (CHO-expressed) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Sequential conditioning-stimulation reveals distinct gene- and stimulus-specific effects of Type I and II IFN on human macrophage functions
    Authors: Q Cheng, F Behzadi, S Sen, S Ohta, R Spreafico, R Teles, RL Modlin, A Hoffmann
    Sci Rep, 2019;9(1):5288.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Lysosomal Protein Lamtor1 Controls Innate Immune Responses via Nuclear Translocation of Transcription Factor EB
    Authors: Y Hayama, T Kimura, Y Takeda, S Nada, S Koyama, H Takamatsu, S Kang, D Ito, Y Maeda, M Nishide, S Nojima, H Sarashina-, T Hosokawa, Y Kinehara, Y Kato, T Nakatani, Y Nakanishi, T Tsuda, T Koba, M Okada, A Kumanogoh
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. How long will recombinant human M-CSF last in cell culture?

    • End-users will need to determine the appropriate concentration and timing when adding Recombinant Human M-CSF Proteins to cell culture experiments. The addition of protein may be dependent on certain culture conditions, including the cell number, density, and media content. For techniques and methodologies, we recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.

  2. Does recombinant human M-CSF show activity in mouse cells?

    • We evaluate the bioactivity of Recombinant Human M-CSF Protein (Catalog # 216-MCC) in a cell proliferation assay using mouse myelogenous leukemia lymphoblast cells. Catalog # 216-MCC exhibits an ED50 in the range of 0.6-2.4 ng/mL in this assay using mouse cells. We also offer the Recombinant Mouse M-CSF Protein (Catalog # 416-ML) for use in mouse assay systems.

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