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Recombinant Human M-CSF (CHO-expressed) Protein

R&D Systems | Catalog # 216-MCC

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human M-CSF (CHO-expressed) Protein (216-MCC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P09603

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all M-CSF Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human M-CSF protein
Glu33-Arg255

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu33

Predicted Molecular Mass

25.1 kDa (monomer)

SDS-PAGE

41-50 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860.
The ED50 for this effect is 0.6-2.4 ng/mL.

Scientific Data Images for Recombinant Human M-CSF (CHO-expressed) Protein

Recombinant Human M-CSF (CHO-expressed) Protein Bioactivity

Recombinant Human M-CSF (CHO-expressed) Protein Bioactivity

Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.6-2.4 ng/mL.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 216-MCC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 216-MCC/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: M-CSF

M-CSF, also known as CSF-1, is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O-glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode
M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of canine, bovine, mouse and rat M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species specific.

References

  1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
  2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
  4. Ryan, G.R. et al. (2001) Blood 98:74.
  5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
  6. Nandi, S. et al. (2006) Blood 107:786.
  7. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
  8. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
  9. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
  10. Koths, K. (1997) Mol. Reprod. Dev. 46:31.
  11. Jang, M-H. et al. (2006) J. Immunol. 177:4055.
  12. Kawasaki, E.S. et al. (1985) Science 230: 291.
  13. Wong, G.G. et al. (1987) Science 235:1504.

Long Name

Macrophage Colony Stimulating Factor

Alternate Names

CSF-1, CSF1, Lanimostim, MCSF

Entrez Gene IDs

1435 (Human); 12977 (Mouse)

Gene Symbol

CSF1

UniProt

P09603

Additional M-CSF Products

Product Documents for Recombinant Human M-CSF (CHO-expressed) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 216-MCC (with carrier)
Download SDS 216-MCC/CF (carrier free)

Product Specific Notices for Recombinant Human M-CSF (CHO-expressed) Protein

For research use only

Citations for Recombinant Human M-CSF (CHO-expressed) Protein

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FAQs for Recombinant Human M-CSF (CHO-expressed) Protein

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Does recombinant human M-CSF show activity in mouse cells?

    A: We evaluate the bioactivity of Recombinant Human M-CSF Protein (Catalog # 216-MCC) in a cell proliferation assay using mouse myelogenous leukemia lymphoblast cells. Catalog # 216-MCC exhibits an ED50 in the range of 0.6-2.4 ng/mL in this assay using mouse cells. We also offer the Recombinant Mouse M-CSF Protein (Catalog # 416-ML) for use in mouse assay systems.

  • Q: How long will recombinant human M-CSF last in cell culture?

    A: End-users will need to determine the appropriate concentration and timing when adding Recombinant Human M-CSF Proteins to cell culture experiments. The addition of protein may be dependent on certain culture conditions, including the cell number, density, and media content. For techniques and methodologies, we recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.

  • Q: Does recombinant human M-CSF show activity in mouse cells?

    A: We evaluate the bioactivity of Recombinant Human M-CSF Protein (Catalog # 216-MCC) in a cell proliferation assay using mouse myelogenous leukemia lymphoblast cells. Catalog # 216-MCC exhibits an ED50 in the range of 0.6-2.4 ng/mL in this assay using mouse cells. We also offer the Recombinant Mouse M-CSF Protein (Catalog # 416-ML) for use in mouse assay systems.

  • Q: How long will recombinant human M-CSF last in cell culture?

    A: End-users will need to determine the appropriate concentration and timing when adding Recombinant Human M-CSF Proteins to cell culture experiments. The addition of protein may be dependent on certain culture conditions, including the cell number, density, and media content. For techniques and methodologies, we recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Proteins and Enzymes