Recombinant Human Noggin Fc Chimera Protein, CF

Newer Version Available: 6057-NG/CF
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Catalog # Availability Size / Price Qty
3344-NG-050
3344-NG-500
3344-NG-100
Graph showing bioactivity of human Noggin protein
1 Image
Product Details
Citations (16)
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Recombinant Human Noggin Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.1-0.4 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP‑4 (Catalog # 314-BP).
Source
Mouse myeloma cell line, NS0-derived human Noggin protein
Human Noggin
(Gln28-Cys232)
Accession # Q13253
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln28 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
49.6 kDa (monomer)
SDS-PAGE
58-62 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3344-NG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Bioactivity of Human Noggin View Larger

Recombinant human Noggin Fc chimera (3344-NG) inhibits recombinant human BMP-4-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 0.1-0.4 µg/mL in the presence of 30 ng/mL of recombinant human BMP-4 (314-BP).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Noggin

Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs) (1, 2). Human Noggin cDNA encodes a 232 amino acid (aa) precursor protein; cleavage of a 19 aa signal peptide generates the 213 aa mature protein which contains an N-terminal acidic region, a central basic heparin‑binding segment and a C-terminal cysteine-knot structure (2). Secreted Noggin probably remains close to the cell surface due to its binding of heparin‑containing proteoglycans (3). Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat, bovine, equine and chicken Noggin, respectively. Noggin binds some BMPs such as BMP-4 with high affinity and others such as BMP-7 with lower affinity, antagonizing BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors (2, 4). During embryogenesis, Noggin antagonizes specific BMPs at defined times during neural tube, somite and cardiomyocyte growth and patterning (5-7). During skeletal development, Noggin prevents chondrocyte hyperplasia, thus allowing proper formation of joints (4). Mutations within the cysteine-knot region of human Noggin are linked to multiple types of skeletal dysplasias that result in apical joint fusions (8). Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin (1). During culture of human embryonic stem cells (hESC) without feeder layers or conditioned medium, but with addition of FGF basic, addition of Noggin to antagonize BMP activity allows hESC to maintain their undifferentiated, pluripotent state (9, 10).

References
  1. Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
  2. Groppe, J. et al. (2002) Nature 420:636.
  3. Paine-Saunders, S et al. (2002) J. Biol. Chem. 277:2089.
  4. Brunet, L. J. et al. (1998) Science 280:1455.
  5. McMahon, J. A. et al. (1998) Genes Dev. 12:1438.
  6. Itsykson, P. et al. (2005) Mol. Cell. Neurosci. 30:24.
  7. Yuasa, S. et al. (2005) Nat. Biotechnol. 23:607.
  8. Gong, Y. et al. (1999) Nat. Genet. 21:302.
  9. Xu, R.-H. et al. (2005) Nat. Methods 2:185.
  10. Wang, G. et al. (2005) Biochem. Biophys. Res. Commun. 330:934.
Entrez Gene IDs
9241 (Human); 18121 (Mouse)
Alternate Names
NOG; Noggin; SYM1; symphalangism 1 (proximal); synostoses (multiple) syndrome 1; SYNS1; SYNS1A

Citations for Recombinant Human Noggin Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Differentiation of Retinal Glial Cells From Human Embryonic Stem Cells by Promoting the Notch Signaling Pathway
    Authors: SH Chung, W Shen, KC Davidson, A Pébay, RCB Wong, B Yau, M Gillies
    Front Cell Neurosci, 2019;13(0):527.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Reprogramming of Fibroblasts to Oligodendrocyte Progenitor-like Cells Using CRISPR/Cas9-Based Synthetic Transcription Factors
    Authors: M Matjusaiti, LJ Wagstaff, A Martella, B Baranowski, C Blin, S Gogolok, A Williams, SM Pollard
    Stem Cell Reports, 2019;13(6):1053-1067.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Lineage commitment of embryonic cells involves MEK1-dependent clearance of pluripotency regulator Ventx2
    Authors: P Scerbo, L Marchal, L Kodjabachi
    Elife, 2017;6(0):.
    Species: Xenopus
    Sample Types: In Vivo
    Applications: In Vivo
  4. BMP4 Signaling Is Able to Induce an Epithelial-Mesenchymal Transition-Like Phenotype in Barrett's Esophagus and Esophageal Adenocarcinoma through Induction of SNAIL2.
    Authors: Christine Kestens, Peter D Siersema, G Johan A Offerhaus, Jantine W P M Van Baal
    PLoS ONE, 2016;0(0):1932-6203.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway.
    Authors: Guezguez A, Pare F, Benoit Y, Basora N, Beaulieu J
    Exp Cell Res, 2014;322(2):355-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
    Authors: Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L
    Cell Transplant, 2012;21(6):1321-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  7. Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells.
    Authors: Kelly OG, Chan MY, Martinson LA
    Nat. Biotechnol., 2011;29(8):750-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Signaling hierarchy regulating human endothelial cell development.
    Authors: Kelly MA, Hirschi KK
    Arterioscler. Thromb. Vasc. Biol., 2009;29(5):718-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
    Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
    FEBS Lett., 2009;583(3):561-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. High-density lipoproteins affect endothelial BMP-signaling by modulating expression of the activin-like kinase receptor 1 and 2.
    Authors: Yao Y, Shao ES, Jumabay M, Shahbazian A, Ji S, Bostrom KI
    Arterioscler. Thromb. Vasc. Biol., 2008;28(12):2266-74.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage.
    Authors: Elkabetz Y, Panagiotakos G, Al Shamy G, Socci ND, Tabar V, Studer L
    Genes Dev., 2008;22(2):152-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. BMP gradients steer nerve growth cones by a balancing act of LIM kinase and Slingshot phosphatase on ADF/cofilin.
    Authors: Wen Z, Han L, Bamburg JR, Shim S, Ming GL, Zheng JQ
    J. Cell Biol., 2007;178(1):107-19.
    Species: Xenopus
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed.
    Authors: McLean AB, D&amp;amp;apos;Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S
    Stem Cells, 2007;25(1):29-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Thalidomide induces limb deformities by perturbing the Bmp/Dkk1/Wnt signaling pathway.
    Authors: Knobloch J, Shaughnessy JD, Ruther U
    FASEB J., 2007;21(7):1410-21.
    Species: Chicken
    Sample Types: In Vivo
    Applications: In Vivo
  15. BMPs restrict the position of premuscle masses in the limb buds by influencing Tcf4 expression.
    Authors: Bonafede A, Kohler T, Rodriguez-Niedenfuhr M, Brand-Saberi B
    Dev. Biol., 2006;299(2):330-44.
    Species: Chicken
    Sample Types: In Ovo
    Applications: In Ovo
  16. Multipotent adult progenitor cells from swine bone marrow.
    Authors: Zeng L, Rahrmann E, Hu Q, Lund T, Sandquist L, Felten M, O'Brien TD, O'Brien TD, Zhang J, Verfaillie C
    Stem Cells, 2006;24(11):2355-66.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay

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