Recombinant Human Noggin Protein Summary
Product Specifications
Gln28-Cys232
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6057-NG
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 250 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
6057-NG/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 250 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Data Images

BG01V human embryonic stem cells were cultured in Mouse Embryonic Fibroblast Conditioned Media supplemented with FGF basic (5 ng/mL). Stem cells were driven into early cells of the neuroectoderm using a 3 day incubation in recombinant human Noggin (25 µg/mL) from either R&D Systems (Lot 1, Lot 2; Catalog # 6057-NG) or from two separate competitors (Competitor 1, Competitor 2). Control cells were not incubated in Noggin (No Noggin). The cells were stained for the early ectoderm marker, Otx2, and the neuroectoderm marker, SOX1. (A) Representative images of SOX1 (green), Otx2 (red), and DAPI (blue) staining in embryonic stem cells differentiated with Noggin from R&D Systems or Noggin from Competitor 2. (B) SOX1+ clusters were quantified under each of the indicated culture conditions. Cells treated with R&D Systems Noggin showed an increase in SOX1+ cells compared to both untreated and competitor-treated cells. R&D Systems Noggin showed consistent differentiation across the lots tested. BG01V human embryonic stem cells are licensed from ViaCyte, Inc.

Recombinant Human Noggin (Catalog # 6057-NG) inhibits BMP-4 induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The activity is approximately 30-fold greater than the top competitor's Noggin.
Reconstitution Calculator
Background: Noggin
Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs) (1, 2). Human Noggin cDNA encodes a 232 amino acid (aa) precursor protein; cleavage of a 19 aa signal peptide generates the 213 aa mature protein which contains an N-terminal acidic region, a central basic heparin‑binding segment and a C-terminal cysteine-knot structure (2). Secreted Noggin probably remains close to the cell surface due to its binding of heparin‑containing proteoglycans (3). Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat, bovine, equine and chicken Noggin, respectively. Noggin binds some BMPs such as BMP-4 with high affinity and others such as BMP-7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors (2, 4). During embryogenesis, Noggin antagonizes specific BMPs at defined times, for example, during neural tube, somite and cardiomyocyte growth and patterning (5-7). During skeletal development, Noggin prevents chondrocyte hyperplasia, thus allowing proper formation of joints (4). Mutations within the cysteine-knot region of human Noggin are linked to multiple types of skeletal dysplasias that result in apical joint fusions (8). Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin (1). During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons (6, 9 - 13). Noggin also appears to maintain adult stem cell populations in-vivo, for example, maintaining neural stem cells within the hippocampus (13).
- Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
- Groppe, J. et al. (2002) Nature 420:636.
- Paine-Saunders, S et al. (2002) J. Biol. Chem. 277:2089.
- Brunet, L. J. et al. (1998) Science 280:1455.
- McMahon, J. A. et al. (1998) Genes Dev. 12:1438.
- Itsykson, P. et al. (2005) Mol. Cell. Neurosci. 30:24.
- Yuasa, S. et al. (2005) Nat. Biotechnol. 23:607.
- Gong, Y. et al. (1999) Nat. Genet. 21:302.
- Xu, R.-H. et al. (2005) Nat. Methods 2:185.
- Wang, G. et al. (2005) Biochem. Biophys. Res. Commun. 330:934.
- Chaturvedi, G. et al. (2009) Cell Prolif. 42:425.
- Chiba, S. et al. (2008) Stem Cells 26:2810.
- Bonaguidi, M.A. et al. (2008) J. Neurosci. 28:9194.
Citations for Recombinant Human Noggin Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
118
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell development
Authors: NK Vinckier, NA Patel, RJ Geusz, A Wang, J Wang, I Matta, AR Harrington, M Wortham, N Wetton, J Wang, US Jhala, MG Rosenfeld, CW Benner, HP Shih, M Sander
Nat Commun, 2020;11(1):2082.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
GATA3 Mediates a Fast, Irreversible Commitment to BMP4-Driven Differentiation in Human Embryonic Stem Cells
Authors: A Gunne-Brad, A Sullivan, B Gharibi, RSM Sheriff, A Maity, YF Wang, A Edwards, M Jiang, M Howell, R Goldstone, R Wollman, P East, SDM Santos
Cell Stem Cell, 2020;26(5):693-706.e9.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Generation of intestinal organoids derived from human pluripotent stem cells for drug testing
Authors: S Yoshida, H Miwa, T Kawachi, S Kume, K Takahashi
Sci Rep, 2020;10(1):5989.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Structurally Conserved Primate LncRNAs Are Transiently Expressed during Human Cortical Differentiation and Influence Cell-Type-Specific Genes
Authors: AR Field, FMJ Jacobs, IT Fiddes, APR Phillips, AM Reyes-Orti, E LaMontagne, L Whitehead, V Meng, JL Rosenkrant, M Olsen, M Hauessler, S Katzman, SR Salama, D Haussler
Stem Cell Reports, 2019;12(2):245-257.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
High-throughput screens for agonists of bone morphogenetic protein (BMP) signaling identify potent benzoxazole compounds
Authors: STJ Bradford, EJ Ranghini, E Grimley, PH Lee, GR Dressler
J. Biol. Chem., 2019;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
BMP signaling mediates glioma stem cell quiescence and confers treatment resistance in glioblastoma
Authors: R Sachdeva, M Wu, K Johnson, H Kim, A Celebre, U Shahzad, MS Graham, JA Kessler, JH Chuang, J Karamchand, M Bredel, R Verhaak, S Das
Sci Rep, 2019;9(1):14569.
Species: Human
Sample Types: Whole Cells
Applications: Tissue Culture -
Excess growth hormone suppresses DNA damage repair in epithelial cells
Authors: V Chesnokova, S Zonis, R Barrett, H Kameda, K Wawrowsky, A Ben-Shlomo, M Yamamoto, J Gleeson, C Bresee, V Gorbunova, S Melmed
JCI Insight, 2019;4(3):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Controlled modelling of human epiblast and amnion development using stem cells
Authors: Y Zheng, X Xue, Y Shao, S Wang, SN Esfahani, Z Li, JM Muncie, JN Lakins, VM Weaver, DL Gumucio, J Fu
Nature, 2019;573(7774):421-425.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Brain-stiffness-mimicking tilapia collagen gel promotes the induction of dorsal cortical neurons from human pluripotent stem cells
Authors: M Iwashita, H Ohta, T Fujisawa, M Cho, M Ikeya, S Kidoaki, Y Kosodo
Sci Rep, 2019;9(1):3068.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Blockade of STAT3 Causes Severe In Vitro and In Vivo Maturation Defects in Intestinal Organoids Derived from Human Embryonic Stem Cells
Authors: KB Jung, O Kwon, MO Lee, H Lee, YS Son, O Habib, JH Oh, HS Cho, CR Jung, J Kim, MY Son
J Clin Med, 2019;8(7):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Patient-derived pancreas-on-a-chip to model cystic fibrosis-related disorders
Authors: K Shik Mun, K Arora, Y Huang, F Yang, S Yarlagadda, Y Ramananda, M Abu-El-Hai, JJ Palermo, BN Appakalai, JD Nathan, AP Naren
Nat Commun, 2019;10(1):3124.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Activin A receptor type 1-mediated BMP signaling regulates RANKL-induced osteoclastogenesis via canonical SMAD signaling pathway
Authors: M Omi, V Kaartinen, Y Mishina
J. Biol. Chem., 2019;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure
Authors: V Lungova, X Chen, Z Wang, C Kendziorsk, SL Thibeault
Nat Commun, 2019;10(1):4161.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Blockade of STAT3 Causes Severe In Vitro and In Vivo Maturation Defects in Intestinal Organoids Derived from Human Embryonic Stem Cells
Authors: KB Jung, O Kwon, MO Lee, H Lee, YS Son, O Habib, JH Oh, HS Cho, CR Jung, J Kim, MY Son
J Clin Med, 2019;8(7):.
Species: Human
Sample Types: Organoid
Applications: Bioassay -
Generation of human antral and fundic gastric organoids from pluripotent stem cells
Authors: TR Broda, KW McCracken, JM Wells
Nat Protoc, 2019;14(1):28-50.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Point mutations in the PDX1 transactivation domain impair human ?-cell development and function
Authors: X Wang, M Sterr, Ansarullah, I Burtscher, A Böttcher, J Beckenbaue, J Siehler, T Meitinger, HU Häring, H Staiger, FM Cernilogar, G Schotta, M Irmler, J Beckers, CVE Wright, M Bakhti, H Lickert
Mol Metab, 2019;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Differentiation -
Generation of lung organoids from human pluripotent stem cells in vitro
Authors: AJ Miller, BR Dye, D Ferrer-Tor, DR Hill, AW Overeem, LD Shea, JR Spence
Nat Protoc, 2019;14(2):518-540.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay -
Noggin depletion in adipocytes promotes obesity in mice
Authors: AM Blázquez-M, M Jumabay, P Rajbhandar, T Sallam, Y Guo, J Yao, L Vergnes, K Reue, L Zhang, Y Yao, AM Fogelman, P Tontonoz, AJ Lusis, X Wu, KI Boström
Mol Metab, 2019;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The Effect of iPS-Derived Neural Progenitors Seeded on Laminin-Coated pHEMA-MOETACl Hydrogel with Dual Porosity in a Rat Model of Chronic Spinal Cord Injury
Authors: J Ruzicka, N Romanyuk, K Jirakova, A Hejcl, O Janouskova, LU Machova, M Bochin, M Pradny, L Vargova, P Jendelova
Cell Transplant, 2019;0(0):9636897188237.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Differential susceptibility of human neural progenitors and neurons to ischaemic injury
Authors: Y Liu, AE Michalska, M Dottori, E Eaton, JM Courtney, A Antonic, DW Howells
Brain Res. Bull., 2019;156(0):25-32.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Rfx6 promotes the differentiation of peptide-secreting enteroendocrine cells while�repressing genetic programs controlling�serotonin production
Authors: J Piccand, C Vagne, F Blot, A Meunier, A Beucher, P Strasser, ML Lund, S Ghimire, L Nivlet, C Lapp, N Petersen, MS Engelstoft, C Thibault-C, C Keime, SJ Correa, V Schreiber, N Molina, TW Schwartz, A De Arcange, G Gradwohl
Mol Metab, 2019;29(0):24-39.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Targeting chemoresistant colorectal cancer via systemic administration of a BMP7 variant
Authors: V Veschi, LR Mangiapane, A Nicotra, S Di Franco, E Scavo, T Apuzzo, DS Sardina, M Fiori, A Benfante, ML Colorito, G Cocorullo, F Giuliante, C Cipolla, G Pistone, MR Bongiorno, A Rizzo, CM Tate, X Wu, S Rowlinson, LF Stancato, M Todaro, R De Maria, G Stassi
Oncogene, 2019;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells
Authors: W Liang, P Han, EH Kim, J Mak, R Zhang, AG Torrente, JI Goldhaber, E Marbán, HC Cho
Stem Cells, 2019;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Organotypic Cocultures of Human Pluripotent Stem Cell Derived-Neurons with Mammalian Inner Ear Hair Cells and Cochlear Nucleus Slices
Authors: T Hyakumura, S McDougall, S Finch, K Needham, M Dottori, BA Nayagam
Stem Cells Int, 2019;2019(0):8419493.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Oligodendrocyte Intrinsic miR-27a Controls Myelination and Remyelination
Authors: A Tripathi, C Volsko, JP Garcia, E Agirre, KC Allan, PJ Tesar, BD Trapp, G Castelo-Br, FJ Sim, R Dutta
Cell Rep, 2019;29(4):904-919.e9.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals
Authors: T Yung, F Poon, M Liang, S Coquenlorg, EC McGaugh, CC Hui, MD Wilson, MC Nostro, TH Kim
Nat Commun, 2019;10(1):4647.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Lysosomal and network alterations in human mucopolysaccharidosis type VII iPSC-derived neurons
Authors: N Bayó-Puxan, AP Terrasso, S Creyssels, D Simão, C Begon-Pesc, M Lavigne, S Salinas, F Bernex, A Bosch, V Kalatzis, T Levade, AM Cuervo, P Lory, A Consiglio, C Brito, EJ Kremer
Sci Rep, 2018;8(1):16644.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Natural molecules induce and synergize to boost expression of the human antimicrobial peptide ?-defensin-3
Authors: E Sechet, E Telford, C Bonamy, PJ Sansonetti, B Sperandio
Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
Species: Human
Sample Types: Organoids
Applications: Bioassay -
Enhanced Utilization of Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids Using Microengineered Chips.
Authors: Michael J Workman, John P Gleeson, Elissa J Troisi, Hannah Q Estrada, S Jordan Kerns, Christopher D Hinojosa, Geraldine A Hamilton, Stephan R Targan, Clive N Svendsen, Robert J Barrett
Cellular and Molecular Gastroenterology and Hepatology, 2018;0(0):2352-345X.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling
Authors: Y Zhang, Y Yang, M Jiang, SX Huang, W Zhang, D Al Alam, S Danopoulos, M Mori, YW Chen, R Balasubram, SM Chuva de S, C Serra, M Bialecka, E Kim, S Lin, ALR Toste de C, PN Riccio, WV Cardoso, X Zhang, HW Snoeck, J Que
Cell Stem Cell, 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
PEG-4MAL hydrogels for human organoid generation, culture, and in vivo delivery
Authors: R Cruz-Acuña, M Quirós, S Huang, D Siuda, JR Spence, A Nusrat, AJ García
Nat Protoc, 2018;0(0):.
Species: Human
Sample Types: Organoids
Applications: Bioassay -
BMP/SMAD pathway promotes neurogenesis of midbrain dopaminergic neurons in vivo and in human induced pluripotent and neural stem cells
Authors: VM Jovanovic, A Salti, H Tilleman, K Zega, MM Jukic, H Zou, RH Friedel, N Prakash, S Blaess, F Edenhofer, C Brodski
J. Neurosci., 2018;0(0):.
Species: Human
Sample Types:
-
Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA
Authors: MA Scavuzzo, J Chmielowie, D Yang, K Wamble, LS Chaboub, L Duraine, B Tepe, SM Glasgow, BR Arenkiel, C Brou, B Deneen, M Borowiak
Cell Rep, 2018;25(13):3811-3827.e7.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Genetically engineered human cortical spheroid models of tuberous sclerosis
Authors: JD Blair, D Hockemeyer, HS Bateup
Nat. Med., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Derivation of phenotypically diverse neural culture from hESC by combining adherent and dissociation methods
Authors: Y Liu, A Antonic, X Yang, N Korte, K Lim, AE Michalska, M Dottori, DW Howells
J. Neurosci. Methods, 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Human Pluripotent Stem-Cell-Derived Cortical Neurons Integrate Functionally into the Lesioned Adult Murine Visual Cortex in an Area-Specific Way
Authors: I Espuny-Cam, KA Michelsen, D Linaro, A Bilheu, S Acosta-Ver, A Herpoel, M Giugliano, A Gaillard, P Vanderhaeg
Cell Rep, 2018;23(9):2732-2743.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Endocrine lineage biases arise in temporally distinct endocrine progenitors during pancreatic morphogenesis
Authors: MA Scavuzzo, MC Hill, J Chmielowie, D Yang, J Teaw, K Sheng, Y Kong, M Bettini, C Zong, JF Martin, M Borowiak
Nat Commun, 2018;9(1):3356.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Self-Renewing Trophoblast Organoids Recapitulate the Developmental Program of the Early Human Placenta
Authors: S Haider, G Meinhardt, L Saleh, V Kunihs, M Gamperl, U Kaindl, A Ellinger, TR Burkard, C Fiala, J Pollheimer, S Mendjan, PA Latos, M Knöfler
Stem Cell Reports, 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Decoding the dynamic DNA methylation and hydroxymethylation landscapes in endodermal lineage intermediates during pancreatic differentiation of hESC
Authors: J Li, X Wu, Y Zhou, M Lee, L Guo, W Han, W Mo, WM Cao, D Sun, R Xie, Y Huang
Nucleic Acids Res., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa
Authors: A Buskin, L Zhu, V Chichagova, B Basu, S Mozaffari-, D Dolan, A Droop, J Collin, R Bronstein, S Mehrotra, M Farkas, G Hilgen, K White, KT Pan, A Treumann, D Hallam, K Bialas, G Chung, C Mellough, Y Ding, N Krasnogor, S Przyborski, S Zwolinski, J Al-Aama, S Alharthi, Y Xu, G Wheway, K Szymanska, M McKibbin, CF Inglehearn, DJ Elliott, S Lindsay, RR Ali, DH Steel, L Armstrong, E Sernagor, H Urlaub, E Pierce, R Lührmann, SN Grellschei, CA Johnson, M Lako
Nat Commun, 2018;9(1):4234.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A transient DMSO treatment increases the differentiation potential of human pluripotent stem cells through the Rb family
Authors: J Li, C Narayanan, J Bian, D Sambo, T Brickler, W Zhang, S Chetty
PLoS ONE, 2018;13(12):e0208110.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Expression of the human antimicrobial peptide ?-defensin-1 is repressed by the EGFR-ERK-MYC axis in colonic epithelial cells
Authors: C Bonamy, E Sechet, A Amiot, A Alam, M Mourez, L Fraisse, PJ Sansonetti, B Sperandio
Sci Rep, 2018;8(1):18043.
Species: Human
Sample Types: Organoids
Applications: Bioassay -
Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1
Authors: B Memon, M Karam, S Al-Khawaga, EM Abdelalim
Stem Cell Res Ther, 2018;9(1):15.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Sequential Application of Discrete Topographical Patterns Enhances Derivation of Functional Mesencephalic Dopaminergic Neurons from Human Induced Pluripotent Stem Cells
Authors: KKB Tan, WWM Lim, C Chai, M Kukumberg, KL Lim, ELK Goh, EKF Yim
Sci Rep, 2018;8(1):9567.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Enhanced Utilization of Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids Using Microengineered Chips
Authors: MJ Workman, JP Gleeson, EJ Troisi, HQ Estrada, SJ Kerns, CD Hinojosa, GA Hamilton, SR Targan, CN Svendsen, RJ Barrett
Cell Mol Gastroenterol Hepatol, 2018;5(4):669-677.e2.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Microbiota-Derived Indole Metabolites Promote Human and Murine Intestinal Homeostasis through Regulation of Interleukin-10 Receptor
Authors: EE Alexeev, JM Lanis, DJ Kao, EL Campbell, CJ Kelly, KD Battista, ME Gerich, BR Jenkins, ST Walk, DJ Kominsky, SP Colgan
Am. J. Pathol., 2018;0(0):.
Species: Human
Sample Types: Organoid
Applications: Bioassay -
Random Mutagenesis, Clonal Events, and Embryonic or Somatic Origin Determine the mtDNA Variant Type and Load in Human Pluripotent Stem�Cells
Authors: F Zambelli, J Mertens, D Dziedzicka, J Sterckx, C Markouli, A Keller, P Tropel, L Jung, S Viville, H Van de Vel, M Geens, S Seneca, K Sermon, C Spits
Stem Cell Reports, 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Genome-wide analysis of PDX1 target genes in human pancreatic progenitors
Authors: X Wang, M Sterr, I Burtscher, S Chen, A Hieronimus, F Machicao, H Staiger, HU Häring, G Lederer, T Meitinger, FM Cernilogar, G Schotta, M Irmler, J Beckers, M Hrab? de A, M Ray, CVE Wright, M Bakhti, H Lickert
Mol Metab, 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
DEPTOR regulates osteogenic differentiation via inhibiting MEG3-mediated activation of BMP4 signaling and is involved in osteoporosis
Authors: S Chen, L Jia, S Zhang, Y Zheng, Y Zhou
Stem Cell Res Ther, 2018;9(1):185.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Cardiotrophic Growth Factor-Driven Induction of Human Muse Cells Into Cardiomyocyte-Like Phenotype
Authors: M Amin, Y Kushida, S Wakao, M Kitada, K Tatsumi, M Dezawa
Cell Transplant, 2018;27(2):285-298.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Identification of direct negative crosstalk between the SLIT2 and Bone Morphogenetic Protein-Gremlin signaling pathways
Authors: KE Tumelty, N Higginson-, X Fan, P Bajaj, KM Knowlton, M Shamashkin, AJ Coyle, W Lu, SP Berasi
J. Biol. Chem., 2018;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Graphene foam as a biocompatible scaffold for culturing human neurons
Authors: GM D'Abaco, C Mattei, B Nasr, EJ Hudson, AJ Alshawaf, G Chana, IP Everall, B Nayagam, M Dottori, E Skafidas
R Soc Open Sci, 2018;5(3):171364.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model
Authors: X Yuan, Z Li, AC Baines, E Gavriilaki, Z Ye, Z Wen, EM Braunstein, LG Biesecker, L Cheng, X Dong, RA Brodsky
PLoS ONE, 2017;12(4):e0174074.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Common genetic variation drives molecular heterogeneity in human iPSCs
Authors: H Kilpinen, A Goncalves, A Leha, V Afzal, K Alasoo, S Ashford, S Bala, D Bensaddek, FP Casale, OJ Culley, P Danecek, A Faulconbri, PW Harrison, A Kathuria, D McCarthy, SA McCarthy, R Meleckyte, Y Memari, N Moens, F Soares, A Mann, I Streeter, CA Agu, A Alderton, R Nelson, S Harper, M Patel, A White, SR Patel, L Clarke, R Halai, CM Kirton, A Kolb-Kokoc, P Beales, E Birney, D Danovi, AI Lamond, WH Ouwehand, L Vallier, FM Watt, R Durbin, O Stegle, DJ Gaffney
Nature, 2017;546(7658):370-375.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Species-specific developmental timing is maintained by pluripotent stem cells ex utero
Authors: C Barry, MT Schmitz, P Jiang, MP Schwartz, BM Duffin, S Swanson, R Bacher, JM Bolin, AL Elwell, BE McIntosh, R Stewart, JA Thomson
Dev. Biol., 2017;423(2):101-110.
Species: Human
Sample Types: Whole Cells
Applications: Differentiation -
Differentiation of Human Pluripotent Stem Cells into Colonic Organoids via Transient Activation of BMP Signaling
Authors: JO Múnera, N Sundaram, SA Rankin, D Hill, C Watson, M Mahe, JE Vallance, NF Shroyer, KL Sinagoga, A Zarzoso-La, JR Hudson, JC Howell, P Chatuvedi, JR Spence, JM Shannon, AM Zorn, MA Helmrath, JM Wells
Cell Stem Cell, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Bone morphogenetic protein signaling mediated by ALK-2 and DLX2 regulates apoptosis in glioma-initiating cells
Authors: E Raja, A Komuro, R Tanabe, S Sakai, Y Ino, N Saito, T Todo, M Morikawa, H Aburatani, D Koinuma, C Iwata, K Miyazono
Oncogene, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Melanoma-Derived iPCCs Show Differential Tumorigenicity and Therapy Response
Authors: M Bernhardt, D Novak, Y Assenov, E Orouji, N Knappe, K Weina, M Reith, L Larribere, C Gebhardt, C Plass, V Umansky, J Utikal
Stem Cell Reports, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Wnt/?-catenin promotes gastric fundus specification in mice and humans
Authors: KW McCracken, E Aihara, B Martin, CM Crawford, T Broda, J Treguier, X Zhang, JM Shannon, MH Montrose, JM Wells
Nature, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Establishment of a refined culture method for rat colon organoids
Authors: H Isshiki, Y Arimura, K Nagaishi, K Kawakami, K Onodera, K Yamashita, Y Naishiro, M Fujimiya, K Imai, Y Shinomura
Biochem. Biophys. Res. Commun., 2017;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors
Authors: KF Cogger, A Sinha, F Sarangi, EC McGaugh, D Saunders, C Dorrell, S Mejia-Guer, Y Aghazadeh, JL Rourke, RA Screaton, M Grompe, PR Streeter, AC Powers, M Brissova, T Kislinger, MC Nostro
Nat Commun, 2017;8(1):331.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A pluripotent stem cell-based model for post-implantation human amniotic sac development
Authors: Y Shao, K Taniguchi, RF Townshend, T Miki, DL Gumucio, J Fu
Nat Commun, 2017;8(1):208.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A right-handed signalling pathway drives heart looping in vertebrates
Authors: OH Ocaña, H Coskun, C Minguillón, P Murawala, EM Tanaka, J Galcerán, R Muñoz-Cháp, MA Nieto
Nature, 2017;549(7670):86-90.
Species: Zebrafish
Sample Types: Whole Tissue
Applications: Bioassay -
Directly Converted Human Fibroblasts Mature to Neurons and Show Long-Term Survival in Adult Rodent Hippocampus
Authors: N Avaliani, U Pfisterer, A Heuer, M Parmar, M Kokaia, M Andersson
Stem Cells Int, 2017;2017(0):5718608.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells
Authors: T Yamashita, Y Miyamoto, Y Bando, T Ono, S Kobayashi, A Doi, T Araki, Y Kato, T Shirakawa, Y Suzuki, J Yamauchi, S Yoshida, N Sato
PLoS ONE, 2017;12(2):e0171947.
Species: Primate
Sample Types: Whole Cells
Applications: Bioassay -
Epigenetic and transcriptional modulation of WDR5, a chromatin remodeling protein, in Huntington's disease human induced pluripotent stem cell (hiPSC) model
Authors: S Baronchell, A La Spada, A Ntai, A Barbieri, P Conforti, GS Jotti, S Redaelli, A Bentivegna, P De Blasio, I Biunno
Mol. Cell. Neurosci., 2017;82(0):46-57.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
REST suppression mediates neural conversion of adult human fibroblasts via microRNA-dependent and -independent pathways
Authors: J Drouin-Oue, S Lau, PL Brattås, D Rylander O, K Pircs, DA Grassi, LM Collins, R Vuono, A Andersson, G Westergren, C Graff, L Minthon, H Toresson, RA Barker, J Jakobsson, M Parmar
EMBO Mol Med, 2017;9(8):1117-1131.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
TRAIL attenuates RANKL-mediated osteoblastic signalling in vascular cell mono-culture and co-culture models
Authors: E Harper, KD Rochfort, H Forde, C Davenport, D Smith, PM Cummins
PLoS ONE, 2017;12(11):e0188192.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Constitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming
Authors: T Ruetz, U Pfisterer, B Di Stefano, J Ashmore, M Beniazza, TV Tian, DF Kaemena, L Tosti, W Tan, JR Manning, E Chantzoura, DR Ottosson, S Collombet, A Johnsson, E Cohen, K Yusa, S Linnarsson, T Graf, M Parmar, K Kaji
Cell Stem Cell, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Zeb2 is a negative regulator of midbrain dopaminergic axon growth and target innervation
Authors: SV Hegarty, SL Wyatt, L Howard, E Stappers, D Huylebroec, AM Sullivan, GW O'Keeffe
Sci Rep, 2017;7(1):8568.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Reductive carboxylation is a major metabolic pathway in the retinal pigment epithelium
Proc. Natl. Acad. Sci. U.S.A., 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Altered neuronal network and rescue in a human MECP2 duplication model
Mol. Psychiatry, 2016;21(2):178-88.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
BMP4/Id2 signaling pathway is a novel therapeutic target for late outgrowth endothelial progenitor cell-mediated endothelial injury repair
Authors: Jun Tao
Int. J. Cardiol., 2016;228(0):796-804.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Growth hormone is permissive for neoplastic colon growth
Proc Natl Acad Sci USA, 2016;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay -
Remyelination Is Correlated with Regulatory T Cell Induction Following Human Embryoid Body-Derived Neural Precursor Cell Transplantation in a Viral Model of Multiple Sclerosis
Authors: Warren C Plaisted
PLoS ONE, 2016;11(6):e0157620.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Early intervention for spinal cord injury with human induced pluripotent stem cells oligodendrocyte progenitors.
Authors: All A, Gharibani P, Gupta S, Bazley F, Pashai N, Chou B, Shah S, Resar L, Cheng L, Gearhart J, Kerr C
PLoS ONE, 2015;10(1):e0116933.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
SMAD4 exerts a tumor-promoting role in hepatocellular carcinoma.
Authors: Hernanda P, Chen K, Das A, Sideras K, Wang W, Li J, Cao W, Bots S, Kodach L, de Man R, Ijzermans J, Janssen H, Stubbs A, Sprengers D, Bruno M, Metselaar H, ten Hagen T, Kwekkeboom J, Peppelenbosch M, Pan Q
Oncogene, 2015;34(39):5055-68.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors.
Authors: Cebola I, Rodriguez-Segui S, Cho C, Bessa J, Rovira M, Luengo M, Chhatriwala M, Berry A, Ponsa-Cobas J, Maestro M, Jennings R, Pasquali L, Moran I, Castro N, Hanley N, Gomez-Skarmeta J, Vallier L, Ferrer J
Nat Cell Biol, 2015;17(5):615-26.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Resveratrol ameliorates the maturation process of beta-cell-like cells obtained from an optimized differentiation protocol of human embryonic stem cells.
Authors: Pezzolla D, Lopez-Beas J, Lachaud C, Dominguez-Rodriguez A, Smani T, Hmadcha A, Soria B
PLoS ONE, 2015;10(3):e0119904.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A dynamic Shh expression pattern, regulated by SHH and BMP signaling, coordinates fusion of primordia in the amniote face.
Authors: Hu D, Young N, Li X, Xu Y, Hallgrimsson B, Marcucio R
Development, 2015;142(3):567-74.
Species: Chicken
Sample Types: Whole Organism
Applications: In Vivo -
In vitro generation of human pluripotent stem cell derived lung organoids.
Authors: Dye, Briana R, Hill, David R, Ferguson, Michael, Tsai, Yu-Hwai, Nagy, Melinda, Dyal, Rachel, Wells, James M, Mayhew, Christop, Nattiv, Roy, Klein, Ophir D, White, Eric S, Deutsch, Gail H, Spence, Jason R
Elife, 2015;4(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Persistence and toxin production by Clostridium difficile within human intestinal organoids result in disruption of epithelial paracellular barrier function.
Authors: Leslie J, Huang S, Opp J, Nagy M, Kobayashi M, Young V, Spence J
Infect Immun, 2015;83(1):138-45.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells.
Authors: Huang S, Green M, de Carvalho A, Mumau M, Chen Y, D'Souza S, Snoeck H
Nat Protoc, 2015;10(3):413-25.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Optimal effector functions in human natural killer cells rely upon autocrine bone morphogenetic protein signaling.
Authors: Robson N, Hidalgo L, McAlpine T, Wei H, Martinez V, Entrena A, Melen G, MacDonald A, Phythian-Adams A, Sacedon R, Maraskovsky E, Cebon J, Ramirez M, Vicente A, Varas A
Cancer Res, 2014;74(18):5019-31.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Retinoic acid signalling regulates the development of tonotopically patterned hair cells in the chicken cochlea.
Authors: Thiede B, Mann Z, Chang W, Ku Y, Son Y, Lovett M, Kelley M, Corwin J
Nat Commun, 2014;5(0):3840.
Species: Chicken
Sample Types: Whole Tissue
Applications: Bioassay -
A gradient of Bmp7 specifies the tonotopic axis in the developing inner ear.
Authors: Mann Z, Thiede B, Chang W, Shin J, May-Simera H, Lovett M, Corwin J, Kelley M
Nat Commun, 2014;5(0):3839.
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay -
Dual small-molecule targeting of SMAD signaling stimulates human induced pluripotent stem cells toward neural lineages.
Authors: Wattanapanitch M, Klincumhom N, Potirat P, Amornpisutt R, Lorthongpanich C, U-pratya Y, Laowtammathron C, Kheolamai P, Poungvarin N, Issaragrisil S
PLoS ONE, 2014;9(9):e106952.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Influence of in vitro and in vivo oxygen modulation on beta cell differentiation from human embryonic stem cells.
Authors: Cechin S, Alvarez-Cubela S, Giraldo J, Molano R, Villate S, Ricordi C, Pileggi A, Inverardi L, Fraker C, Dominguez-Bendala J
Stem Cells Transl Med, 2014;3(3):277-89.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
CEP290 gene transfer rescues Leber congenital amaurosis cellular phenotype.
Authors: Burnight E, Wiley L, Drack A, Braun T, Anfinson K, Kaalberg E, Halder J, Affatigato L, Mullins R, Stone E, Tucker B
Gene Ther, 2014;21(7):662-72.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Primary cilia in stem cells and neural progenitors are regulated by neutral sphingomyelinase 2 and ceramide.
Authors: He Q, Wang G, Wakade S, Dasgupta S, Dinkins M, Kong J, Spassieva S, Bieberich E
Mol Biol Cell, 2014;25(11):1715-29.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Real-time discrimination between proliferation and neuronal and astroglial differentiation of human neural stem cells.
Authors: Lee, Rimi, Kim, Il-Sun, Han, Nalae, Yun, Seokhwan, Park, Kook In, Yoo, Kyung-Hw
Sci Rep, 2014;4(0):6319.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Highly efficient generation of induced neurons from human fibroblasts that survive transplantation into the adult rat brain.
Authors: Pereira, Maria, Pfisterer, Ulrich, Rylander, Daniella, Torper, Olof, Lau, Shong, Lundblad, Martin, Grealish, Shane, Parmar, Malin
Sci Rep, 2014;4(0):6330.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Selective lentiviral gene delivery to CD133-expressing human glioblastoma stem cells.
Authors: Bayin, N Sumru, Modrek, Aram S, Dietrich, August, Lebowitz, Jonathan, Abel, Tobias, Song, Hae-Ri, Schober, Markus, Zagzag, David, Buchholz, Christia, Chao, Moses V, Placantonakis, Dimitris
PLoS ONE, 2014;9(12):e116114.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Recessive mutations in a distal PTF1A enhancer cause isolated pancreatic agenesis.
Authors: Weedon M, Cebola I, Patch A, Flanagan S, De Franco E, Caswell R, Rodriguez-Segui S, Shaw-Smith C, Cho C, Lango Allen H, Houghton J, Roth C, Chen R, Hussain K, Marsh P, Vallier L, Murray A, Ellard S, Ferrer J, Hattersley A
Nat Genet, 2014;46(1):61-4.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Bone morphogenetic protein 2 signaling negatively modulates lymphatic development in vertebrate embryos.
Authors: Dunworth, William, Cardona-Costa, Jose, Bozkulak, Esra Cag, Kim, Jun-Dae, Meadows, Stryder, Fischer, Johanna, Wang, Yeqi, Cleaver, Ondine, Qyang, Yibing, Ober, Elke A, Jin, Suk-Won
Circ Res, 2014;114(1):56-66.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture.
Authors: Takeuchi, Hiroki, Nakatsuji, Norio, Suemori, Hirofumi
Sci Rep, 2014;4(0):4488.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Inner ear hair cell-like cells from human embryonic stem cells.
Authors: Ronaghi, Mohammad, Nasr, Marjan, Ealy, Megan, Durruthy-Durruthy, Robert, Waldhaus, Joerg, Diaz, Giovanni, Joubert, Lydia-Ma, Oshima, Kazuo, Heller, Stefan
Stem Cells Dev, 2014;23(11):1275-84.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Rapid and efficient differentiation of human pluripotent stem cells into intermediate mesoderm that forms tubules expressing kidney proximal tubular markers.
Authors: Lam A, Freedman B, Morizane R, Lerou P, Valerius M, Bonventre J
J Am Soc Nephrol, 2014;25(6):1211-25.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Defined human pluripotent stem cell culture enables highly efficient neuroepithelium derivation without small molecule inhibitors.
Authors: Lippmann E, Estevez-Silva M, Ashton R
Stem Cells, 2014;32(4):1032-42.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Polycomb group protein expression during differentiation of human embryonic stem cells into pancreatic lineage in vitro.
Authors: Pethe, Prasad, Nagvenkar, Punam, Bhartiya, Deepa
BMC Cell Biol, 2014;15(0):18.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Inhibition of bone morphogenic protein 4 restores endothelial function in db/db diabetic mice.
Authors: Zhang Y, Liu J, Tian X, Wong W, Chen Y, Wang L, Luo J, Cheang W, Lau C, Kwan K, Wang N, Yao X, Huang Y
Arterioscler Thromb Vasc Biol, 2014;34(1):152-9.
Species: Mouse
Sample Types: Whole Organism
Applications: In Vivo -
Prospectively isolated NGN3-expressing progenitors from human embryonic stem cells give rise to pancreatic endocrine cells.
Authors: Cai, Qing, Bonfanti, Paola, Sambathkumar, Rangaraj, Vanuytsel, Kim, Vanhove, Jolien, Gysemans, Conny, Debiec-Rychter, Maria, Raitano, Susanna, Heimberg, Harry, Ordovas, Laura, Verfaillie, Catherin
Stem Cells Transl Med, 2014;3(4):489-99.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A synthetic peptide-acrylate surface for production of insulin-producing cells from human embryonic stem cells.
Authors: Lin P, Hung S, Yang Y, Liao L, Hsieh Y, Yen H, Lu H, Lee M, Chu I, Hwang S
Stem Cells Dev, 2014;23(4):372-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Rapid and efficient directed differentiation of human pluripotent stem cells into retinal pigmented epithelium.
Authors: Buchholz, David E, Pennington, Britney, Croze, Roxanne, Hinman, Cassidy, Coffey, Peter J, Clegg, Dennis O
Stem Cells Transl Med, 2013;2(5):384-93.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Bone morphogenetic protein-2/-4 upregulation promoted by endothelial cells in coculture enhances mouse embryoid body differentiation.
Authors: Talavera-Adame, Dodanim, Gupta, Ankur, Kurtovic, Silvia, Chaiboonma, Kira L, Arumugaswami, Vaithili, Dafoe, Donald C
Stem Cells Dev, 2013;22(24):3252-60.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Directed differentiation and functional maturation of cortical interneurons from human embryonic stem cells.
Authors: Maroof A, Keros S, Tyson J, Ying S, Ganat Y, Merkle F, Liu B, Goulburn A, Stanley E, Elefanty A, Widmer H, Eggan K, Goldstein P, Anderson S, Studer L
Cell Stem Cell, 2013;12(5):559-72.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Patient-specific iPSC-derived photoreceptor precursor cells as a means to investigate retinitis pigmentosa.
Authors: Tucker, Budd A, Mullins, Robert F, Streb, Luan M, Anfinson, Kristin, Eyestone, Mari E, Kaalberg, Emily, Riker, Megan J, Drack, Arlene V, Braun, Terry A, Stone, Edwin M
Elife, 2013;2(0):e00824.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions.
Authors: Brafman D, Phung C, Kumar N, Willert K
Cell Death Differ, 2013;20(3):369-81.
-
Activation of the type I interferon pathway is enhanced in response to human neuronal differentiation.
Authors: Farmer, Jocelyn, Altschaefl, Kate M, O'Shea, K Sue, Miller, David J
PLoS ONE, 2013;8(3):e58813.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Generation of multipotent foregut stem cells from human pluripotent stem cells.
Authors: Hannan, Nicholas, Fordham, Robert P, Syed, Yasir A, Moignard, Victoria, Berry, Andrew, Bautista, Ruben, Hanley, Neil A, Jensen, Kim B, Vallier, Ludovic
Stem Cell Reports, 2013;1(4):293-306.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Enrichment of human embryonic stem cell-derived NKX6.1-expressing pancreatic progenitor cells accelerates the maturation of insulin-secreting cells in vivo.
Authors: Rezania A, Bruin J, Xu J, Narayan K, Fox J, O'Neil J, Kieffer T
Stem Cells, 2013;31(11):2432-42.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Cited2 gene controls pluripotency and cardiomyocyte differentiation of murine embryonic stem cells through Oct4 gene.
Authors: Li Q, Ramirez-Bergeron D, Dunwoodie S, Yang Y
J Biol Chem, 2012;287(34):29088-100.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interplay between electrical activity and bone morphogenetic protein signaling regulates spinal neuron differentiation.
Authors: Swapna, Immani, Borodinsky, Laura N
Proc Natl Acad Sci U S A, 2012;109(40):16336-41.
Species: Xenopus
Sample Types: Whole Tissue
Applications: Bioassay -
Downregulation of hemojuvelin prevents inhibitory effects of bone morphogenetic proteins on iron metabolism in hepatocellular carcinoma.
Authors: Maegdefrau U, Arndt S, Kivorski G, Hellerbrand C, Bosserhoff AK
Lab. Invest., 2011;91(11):1615-23.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.
Authors: Spence JR, Mayhew CN, Rankin SA
Nature, 2011;470(7332):105-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
Authors: Klim JR, Li L, Wrighton PJ
Nat. Methods, 2010;7(12):989-94.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Asynchronous mixing of kidney progenitor cells potentiates nephrogenesis in organoids.
Authors: Kumar Gupta A, Sarkar P, Wertheim J, Pan X, Carroll T, Oxburgh L
Commun Biol, 0;3(1):231.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Translating dosage compensation to trisomy 21.
Authors: Jiang J, Jing Y, Cost G, Chiang J, Kolpa H, Cotton A, Carone D, Carone B, Shivak D, Guschin D, Pearl J, Rebar E, Byron M, Gregory P, Brown C, Urnov F, Hall L, Lawrence J
Nature, 0;500(7462):296-300.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
-
What is the recommended method for reconstitution of a lyophilized protein or antibody?
Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand. Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.
-
Are R&D Systems recombinant proteins and antibodies sterile?
Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
-
What is the half life of Recombinant Human Noggin Protein (Catalog # 6057-NG) in vitro?
Recombinant Human Noggin Protein (Catalog # 6057-NG) is evaluated for bioactivity by its ability to inhibit BMP-4-induced alkaline phosphatase production in mouse chondrogenic cells in vitro. We have not evaluated the half-life of 6057-NG. The half-life of a specific protein will depend on experimental conditions, including the cell number, density, and media content. It is up to the end-users to determine the appropriate concentration and timing when adding 6057-NG to individual experiments. For techniques and methodologies, we recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.
-
Has Recombinant Human Noggin Protein (Catalog # 6057-NG or 6057-NG/CF) been eavaluated in vivo?
Recombinant Human Noggin Protein (Catalog # 6057-NG) is evaluated for bioactivity by its ability to inhibit BMP-4-induced alkaline phosphatase production in mouse chondrogenic cells in vitro. We have not evaluated this protein for activity in vivo, but we are aware of publications citing use of the protein this type of experimentation. We recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.
Animal-Free Recombinant Proteins
GMP Recombinant Proteins
ProDots Proteins
Recombinant Proteins
Tocris Peptides
Tocris Small Molecules
Reviews for Recombinant Human Noggin Protein
Average Rating: 4.4 (Based on 7 Reviews)
Have you used Recombinant Human Noggin Protein?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Reason for Rating: Cells grew well.
combination of a low dose of Noggin and the bone morphogenetic protein